Genetic tool development in marine protists: emerging model organisms for experimental cell biology

Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span the diversity of the eukaryotic tree of life. However, genetic tractability has been limited to a few species, which do not represent eukaryotic diversity or environmentally relevant...

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Detalles Bibliográficos
Autores principales: Faktorová, Drahomíra, Nisbet, R. Ellen R., Fernández Robledo, José A., Casacuberta, Elena, Sudek, Lisa, Allen, Andrew E., Ares, Manuel, Aresté, Cristina, Balestreri, Cecilia, Barbrook, Adrian C., Beardslee, Patrick, Bender, Sara, Booth, David S., Bouget, François-Yves, Bowler, Chris, Breglia, Susana A., Brownlee, Colin, Burger, Gertraud, Cerutti, Heriberto, Cesaroni, Rachele, Chiurillo, Miguel A., Clemente, Thomas, Coles, Duncan B., Collier, Jackie L., Cooney, Elizabeth C., Coyne, Kathryn, Docampo, Roberto, Dupont, Christopher L., Edgcomb, Virginia, Einarsson, Elin, Elustondo, Pía A., Federici, Fernan, Freire-Beneitez, Veronica, Freyria, Nastasia J., Fukuda, Kodai, García, Paulo A., Girguis, Peter R., Gomaa, Fatma, Gornik, Sebastian G., Guo, Jian, Hampl, Vladimír, Hanawa, Yutaka, Haro-Contreras, Esteban R., Hehenberger, Elisabeth, Highfield, Andrea, Hirakawa, Yoshihisa, Hopes, Amanda, Howe, Christopher J., Hu, Ian, Ibañez, Jorge, Irwin, Nicholas A. T., Ishii, Yuu, Janowicz, Natalia Ewa, Jones, Adam C., Kachale, Ambar, Fujimura-Kamada, Konomi, Kaur, Binnypreet, Kaye, Jonathan Z., Kazana, Eleanna, Keeling, Patrick J., King, Nicole, Klobutcher, Lawrence A., Lander, Noelia, Lassadi, Imen, Li, Zhuhong, Lin, Senjie, Lozano, Jean-Claude, Luan, Fulei, Maruyama, Shinichiro, Matute, Tamara, Miceli, Cristina, Minagawa, Jun, Moosburner, Mark, Najle, Sebastián R., Nanjappa, Deepak, Nimmo, Isabel C., Noble, Luke, Novák Vanclová, Anna M. G., Nowacki, Mariusz, Nuñez, Isaac, Pain, Arnab, Piersanti, Angela, Pucciarelli, Sandra, Pyrih, Jan, Rest, Joshua S., Rius, Mariana, Robertson, Deborah, Ruaud, Albane, Ruiz-Trillo, Iñaki, Sigg, Monika A., Silver, Pamela A., Slamovits, Claudio H., Jason Smith, G., Sprecher, Brittany N., Stern, Rowena, Swart, Estienne C., Tsaousis, Anastasios D., Tsypin, Lev, Turkewitz, Aaron, Turnšek, Jernej, Valach, Matus, Vergé, Valérie, von Dassow, Peter, von der Haar, Tobias, Waller, Ross F., Wang, Lu, Wen, Xiaoxue, Wheeler, Glen, Woods, April, Zhang, Huan, Mock, Thomas, Worden, Alexandra Z., Lukeš, Julius
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group US 2020
Materias:
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7200600/
https://www.ncbi.nlm.nih.gov/pubmed/32251396
http://dx.doi.org/10.1038/s41592-020-0796-x
Descripción
Sumario:Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span the diversity of the eukaryotic tree of life. However, genetic tractability has been limited to a few species, which do not represent eukaryotic diversity or environmentally relevant taxa. Here, we report on the development of genetic tools in a range of protists primarily from marine environments. We present evidence for foreign DNA delivery and expression in 13 species never before transformed and for advancement of tools for eight other species, as well as potential reasons for why transformation of yet another 17 species tested was not achieved. Our resource in genetic manipulation will provide insights into the ancestral eukaryotic lifeforms, general eukaryote cell biology, protein diversification and the evolution of cellular pathways.

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