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Genetic tool development in marine protists: emerging model organisms for experimental cell biology
Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span the diversity of the eukaryotic tree of life. However, genetic tractability has been limited to a few species, which do not represent eukaryotic diversity or environmentally relevant...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group US
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7200600/ https://www.ncbi.nlm.nih.gov/pubmed/32251396 http://dx.doi.org/10.1038/s41592-020-0796-x |
| _version_ | 1783529368104992768 |
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| author | Faktorová, Drahomíra Nisbet, R. Ellen R. Fernández Robledo, José A. Casacuberta, Elena Sudek, Lisa Allen, Andrew E. Ares, Manuel Aresté, Cristina Balestreri, Cecilia Barbrook, Adrian C. Beardslee, Patrick Bender, Sara Booth, David S. Bouget, François-Yves Bowler, Chris Breglia, Susana A. Brownlee, Colin Burger, Gertraud Cerutti, Heriberto Cesaroni, Rachele Chiurillo, Miguel A. Clemente, Thomas Coles, Duncan B. Collier, Jackie L. Cooney, Elizabeth C. Coyne, Kathryn Docampo, Roberto Dupont, Christopher L. Edgcomb, Virginia Einarsson, Elin Elustondo, Pía A. Federici, Fernan Freire-Beneitez, Veronica Freyria, Nastasia J. Fukuda, Kodai García, Paulo A. Girguis, Peter R. Gomaa, Fatma Gornik, Sebastian G. Guo, Jian Hampl, Vladimír Hanawa, Yutaka Haro-Contreras, Esteban R. Hehenberger, Elisabeth Highfield, Andrea Hirakawa, Yoshihisa Hopes, Amanda Howe, Christopher J. Hu, Ian Ibañez, Jorge Irwin, Nicholas A. T. Ishii, Yuu Janowicz, Natalia Ewa Jones, Adam C. Kachale, Ambar Fujimura-Kamada, Konomi Kaur, Binnypreet Kaye, Jonathan Z. Kazana, Eleanna Keeling, Patrick J. King, Nicole Klobutcher, Lawrence A. Lander, Noelia Lassadi, Imen Li, Zhuhong Lin, Senjie Lozano, Jean-Claude Luan, Fulei Maruyama, Shinichiro Matute, Tamara Miceli, Cristina Minagawa, Jun Moosburner, Mark Najle, Sebastián R. Nanjappa, Deepak Nimmo, Isabel C. Noble, Luke Novák Vanclová, Anna M. G. Nowacki, Mariusz Nuñez, Isaac Pain, Arnab Piersanti, Angela Pucciarelli, Sandra Pyrih, Jan Rest, Joshua S. Rius, Mariana Robertson, Deborah Ruaud, Albane Ruiz-Trillo, Iñaki Sigg, Monika A. Silver, Pamela A. Slamovits, Claudio H. Jason Smith, G. Sprecher, Brittany N. Stern, Rowena Swart, Estienne C. Tsaousis, Anastasios D. Tsypin, Lev Turkewitz, Aaron Turnšek, Jernej Valach, Matus Vergé, Valérie von Dassow, Peter von der Haar, Tobias Waller, Ross F. Wang, Lu Wen, Xiaoxue Wheeler, Glen Woods, April Zhang, Huan Mock, Thomas Worden, Alexandra Z. Lukeš, Julius |
| author_facet | Faktorová, Drahomíra Nisbet, R. Ellen R. Fernández Robledo, José A. Casacuberta, Elena Sudek, Lisa Allen, Andrew E. Ares, Manuel Aresté, Cristina Balestreri, Cecilia Barbrook, Adrian C. Beardslee, Patrick Bender, Sara Booth, David S. Bouget, François-Yves Bowler, Chris Breglia, Susana A. Brownlee, Colin Burger, Gertraud Cerutti, Heriberto Cesaroni, Rachele Chiurillo, Miguel A. Clemente, Thomas Coles, Duncan B. Collier, Jackie L. Cooney, Elizabeth C. Coyne, Kathryn Docampo, Roberto Dupont, Christopher L. Edgcomb, Virginia Einarsson, Elin Elustondo, Pía A. Federici, Fernan Freire-Beneitez, Veronica Freyria, Nastasia J. Fukuda, Kodai García, Paulo A. Girguis, Peter R. Gomaa, Fatma Gornik, Sebastian G. Guo, Jian Hampl, Vladimír Hanawa, Yutaka Haro-Contreras, Esteban R. Hehenberger, Elisabeth Highfield, Andrea Hirakawa, Yoshihisa Hopes, Amanda Howe, Christopher J. Hu, Ian Ibañez, Jorge Irwin, Nicholas A. T. Ishii, Yuu Janowicz, Natalia Ewa Jones, Adam C. Kachale, Ambar Fujimura-Kamada, Konomi Kaur, Binnypreet Kaye, Jonathan Z. Kazana, Eleanna Keeling, Patrick J. King, Nicole Klobutcher, Lawrence A. Lander, Noelia Lassadi, Imen Li, Zhuhong Lin, Senjie Lozano, Jean-Claude Luan, Fulei Maruyama, Shinichiro Matute, Tamara Miceli, Cristina Minagawa, Jun Moosburner, Mark Najle, Sebastián R. Nanjappa, Deepak Nimmo, Isabel C. Noble, Luke Novák Vanclová, Anna M. G. Nowacki, Mariusz Nuñez, Isaac Pain, Arnab Piersanti, Angela Pucciarelli, Sandra Pyrih, Jan Rest, Joshua S. Rius, Mariana Robertson, Deborah Ruaud, Albane Ruiz-Trillo, Iñaki Sigg, Monika A. Silver, Pamela A. Slamovits, Claudio H. Jason Smith, G. Sprecher, Brittany N. Stern, Rowena Swart, Estienne C. Tsaousis, Anastasios D. Tsypin, Lev Turkewitz, Aaron Turnšek, Jernej Valach, Matus Vergé, Valérie von Dassow, Peter von der Haar, Tobias Waller, Ross F. Wang, Lu Wen, Xiaoxue Wheeler, Glen Woods, April Zhang, Huan Mock, Thomas Worden, Alexandra Z. Lukeš, Julius |
| author_sort | Faktorová, Drahomíra |
| collection | PubMed |
| description | Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span the diversity of the eukaryotic tree of life. However, genetic tractability has been limited to a few species, which do not represent eukaryotic diversity or environmentally relevant taxa. Here, we report on the development of genetic tools in a range of protists primarily from marine environments. We present evidence for foreign DNA delivery and expression in 13 species never before transformed and for advancement of tools for eight other species, as well as potential reasons for why transformation of yet another 17 species tested was not achieved. Our resource in genetic manipulation will provide insights into the ancestral eukaryotic lifeforms, general eukaryote cell biology, protein diversification and the evolution of cellular pathways. |
| format | Online Article Text |
| id | pubmed-7200600 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2020 |
| publisher | Nature Publishing Group US |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-72006002020-05-07 Genetic tool development in marine protists: emerging model organisms for experimental cell biology Faktorová, Drahomíra Nisbet, R. Ellen R. Fernández Robledo, José A. Casacuberta, Elena Sudek, Lisa Allen, Andrew E. Ares, Manuel Aresté, Cristina Balestreri, Cecilia Barbrook, Adrian C. Beardslee, Patrick Bender, Sara Booth, David S. Bouget, François-Yves Bowler, Chris Breglia, Susana A. Brownlee, Colin Burger, Gertraud Cerutti, Heriberto Cesaroni, Rachele Chiurillo, Miguel A. Clemente, Thomas Coles, Duncan B. Collier, Jackie L. Cooney, Elizabeth C. Coyne, Kathryn Docampo, Roberto Dupont, Christopher L. Edgcomb, Virginia Einarsson, Elin Elustondo, Pía A. Federici, Fernan Freire-Beneitez, Veronica Freyria, Nastasia J. Fukuda, Kodai García, Paulo A. Girguis, Peter R. Gomaa, Fatma Gornik, Sebastian G. Guo, Jian Hampl, Vladimír Hanawa, Yutaka Haro-Contreras, Esteban R. Hehenberger, Elisabeth Highfield, Andrea Hirakawa, Yoshihisa Hopes, Amanda Howe, Christopher J. Hu, Ian Ibañez, Jorge Irwin, Nicholas A. T. Ishii, Yuu Janowicz, Natalia Ewa Jones, Adam C. Kachale, Ambar Fujimura-Kamada, Konomi Kaur, Binnypreet Kaye, Jonathan Z. Kazana, Eleanna Keeling, Patrick J. King, Nicole Klobutcher, Lawrence A. Lander, Noelia Lassadi, Imen Li, Zhuhong Lin, Senjie Lozano, Jean-Claude Luan, Fulei Maruyama, Shinichiro Matute, Tamara Miceli, Cristina Minagawa, Jun Moosburner, Mark Najle, Sebastián R. Nanjappa, Deepak Nimmo, Isabel C. Noble, Luke Novák Vanclová, Anna M. G. Nowacki, Mariusz Nuñez, Isaac Pain, Arnab Piersanti, Angela Pucciarelli, Sandra Pyrih, Jan Rest, Joshua S. Rius, Mariana Robertson, Deborah Ruaud, Albane Ruiz-Trillo, Iñaki Sigg, Monika A. Silver, Pamela A. Slamovits, Claudio H. Jason Smith, G. Sprecher, Brittany N. Stern, Rowena Swart, Estienne C. Tsaousis, Anastasios D. Tsypin, Lev Turkewitz, Aaron Turnšek, Jernej Valach, Matus Vergé, Valérie von Dassow, Peter von der Haar, Tobias Waller, Ross F. Wang, Lu Wen, Xiaoxue Wheeler, Glen Woods, April Zhang, Huan Mock, Thomas Worden, Alexandra Z. Lukeš, Julius Nat Methods Resource Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span the diversity of the eukaryotic tree of life. However, genetic tractability has been limited to a few species, which do not represent eukaryotic diversity or environmentally relevant taxa. Here, we report on the development of genetic tools in a range of protists primarily from marine environments. We present evidence for foreign DNA delivery and expression in 13 species never before transformed and for advancement of tools for eight other species, as well as potential reasons for why transformation of yet another 17 species tested was not achieved. Our resource in genetic manipulation will provide insights into the ancestral eukaryotic lifeforms, general eukaryote cell biology, protein diversification and the evolution of cellular pathways. Nature Publishing Group US 2020-04-06 2020 /pmc/articles/PMC7200600/ /pubmed/32251396 http://dx.doi.org/10.1038/s41592-020-0796-x Text en © The Author(s), under exclusive licence to Springer Nature America, Inc. 2020 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
| spellingShingle | Resource Faktorová, Drahomíra Nisbet, R. Ellen R. Fernández Robledo, José A. Casacuberta, Elena Sudek, Lisa Allen, Andrew E. Ares, Manuel Aresté, Cristina Balestreri, Cecilia Barbrook, Adrian C. Beardslee, Patrick Bender, Sara Booth, David S. Bouget, François-Yves Bowler, Chris Breglia, Susana A. Brownlee, Colin Burger, Gertraud Cerutti, Heriberto Cesaroni, Rachele Chiurillo, Miguel A. Clemente, Thomas Coles, Duncan B. Collier, Jackie L. Cooney, Elizabeth C. Coyne, Kathryn Docampo, Roberto Dupont, Christopher L. Edgcomb, Virginia Einarsson, Elin Elustondo, Pía A. Federici, Fernan Freire-Beneitez, Veronica Freyria, Nastasia J. Fukuda, Kodai García, Paulo A. Girguis, Peter R. Gomaa, Fatma Gornik, Sebastian G. Guo, Jian Hampl, Vladimír Hanawa, Yutaka Haro-Contreras, Esteban R. Hehenberger, Elisabeth Highfield, Andrea Hirakawa, Yoshihisa Hopes, Amanda Howe, Christopher J. Hu, Ian Ibañez, Jorge Irwin, Nicholas A. T. Ishii, Yuu Janowicz, Natalia Ewa Jones, Adam C. Kachale, Ambar Fujimura-Kamada, Konomi Kaur, Binnypreet Kaye, Jonathan Z. Kazana, Eleanna Keeling, Patrick J. King, Nicole Klobutcher, Lawrence A. Lander, Noelia Lassadi, Imen Li, Zhuhong Lin, Senjie Lozano, Jean-Claude Luan, Fulei Maruyama, Shinichiro Matute, Tamara Miceli, Cristina Minagawa, Jun Moosburner, Mark Najle, Sebastián R. Nanjappa, Deepak Nimmo, Isabel C. Noble, Luke Novák Vanclová, Anna M. G. Nowacki, Mariusz Nuñez, Isaac Pain, Arnab Piersanti, Angela Pucciarelli, Sandra Pyrih, Jan Rest, Joshua S. Rius, Mariana Robertson, Deborah Ruaud, Albane Ruiz-Trillo, Iñaki Sigg, Monika A. Silver, Pamela A. Slamovits, Claudio H. Jason Smith, G. Sprecher, Brittany N. Stern, Rowena Swart, Estienne C. Tsaousis, Anastasios D. Tsypin, Lev Turkewitz, Aaron Turnšek, Jernej Valach, Matus Vergé, Valérie von Dassow, Peter von der Haar, Tobias Waller, Ross F. Wang, Lu Wen, Xiaoxue Wheeler, Glen Woods, April Zhang, Huan Mock, Thomas Worden, Alexandra Z. Lukeš, Julius Genetic tool development in marine protists: emerging model organisms for experimental cell biology |
| title | Genetic tool development in marine protists: emerging model organisms for experimental cell biology |
| title_full | Genetic tool development in marine protists: emerging model organisms for experimental cell biology |
| title_fullStr | Genetic tool development in marine protists: emerging model organisms for experimental cell biology |
| title_full_unstemmed | Genetic tool development in marine protists: emerging model organisms for experimental cell biology |
| title_short | Genetic tool development in marine protists: emerging model organisms for experimental cell biology |
| title_sort | genetic tool development in marine protists: emerging model organisms for experimental cell biology |
| topic | Resource |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7200600/ https://www.ncbi.nlm.nih.gov/pubmed/32251396 http://dx.doi.org/10.1038/s41592-020-0796-x |
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