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miR-124 Intensified Oxaliplatin-Based Chemotherapy by Targeting CAPN2 in Colorectal Cancer
Our previous study demonstrated that miR-124 was downregulated in colorectal cancer (CRC) compared with normal mucosa, and the downregulated expression of miR-124 was an independent prognostic factor in CRC patients. However, the function of miR-124 in CRC patients treated with chemotherapy is curre...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society of Gene & Cell Therapy
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7200624/ https://www.ncbi.nlm.nih.gov/pubmed/32382656 http://dx.doi.org/10.1016/j.omto.2020.04.003 |
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author | Xie, Xu-Qin Wang, Mo-Jin Li, Yuan Lei, Lin-Ping Wang, Ning Lv, Zhao-Ying Chen, Ke-Ling Zhou, Bin Ping, Jie Zhou, Zong-Guang Sun, Xiao-Feng |
author_facet | Xie, Xu-Qin Wang, Mo-Jin Li, Yuan Lei, Lin-Ping Wang, Ning Lv, Zhao-Ying Chen, Ke-Ling Zhou, Bin Ping, Jie Zhou, Zong-Guang Sun, Xiao-Feng |
author_sort | Xie, Xu-Qin |
collection | PubMed |
description | Our previous study demonstrated that miR-124 was downregulated in colorectal cancer (CRC) compared with normal mucosa, and the downregulated expression of miR-124 was an independent prognostic factor in CRC patients. However, the function of miR-124 in CRC patients treated with chemotherapy is currently unclear. The aim of this study was to determine the miR-124 expression and its regulative role in oxaliplatin (L-OHP)-based chemotherapy of CRC patients. We observed that low miR-124 expression was correlated with worse overall survival (OS) in the 220 patients who received postoperative chemotherapy of 5-fluorouracil [5-FU]+leucovorin+L-OHP (FOLFOX) or capecitabine+L-OHP (XELOX). miR-124 overexpression promoted L-OHP-induced, but not 5-FU-induced, cytotoxicity and apoptosis in HT29 and SW480 cells. CAPN2 was a direct target of miR-124, and its protein expression was reduced by forced expression of miR-124. miR-124 inhibited tumorigenesis and promoted OS of mice bearing xenograft tumors, especially upon L-OHP treatment. miR-124 also promoted L-OHP-induced apoptosis and restrained CAPN2 protein expression in xenograft tumors. Our results suggest that miR-124 could be considered as both a predictor of L-OHP-based chemotherapy for personalized treatment and a therapeutic target for CRC. |
format | Online Article Text |
id | pubmed-7200624 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American Society of Gene & Cell Therapy |
record_format | MEDLINE/PubMed |
spelling | pubmed-72006242020-05-07 miR-124 Intensified Oxaliplatin-Based Chemotherapy by Targeting CAPN2 in Colorectal Cancer Xie, Xu-Qin Wang, Mo-Jin Li, Yuan Lei, Lin-Ping Wang, Ning Lv, Zhao-Ying Chen, Ke-Ling Zhou, Bin Ping, Jie Zhou, Zong-Guang Sun, Xiao-Feng Mol Ther Oncolytics Article Our previous study demonstrated that miR-124 was downregulated in colorectal cancer (CRC) compared with normal mucosa, and the downregulated expression of miR-124 was an independent prognostic factor in CRC patients. However, the function of miR-124 in CRC patients treated with chemotherapy is currently unclear. The aim of this study was to determine the miR-124 expression and its regulative role in oxaliplatin (L-OHP)-based chemotherapy of CRC patients. We observed that low miR-124 expression was correlated with worse overall survival (OS) in the 220 patients who received postoperative chemotherapy of 5-fluorouracil [5-FU]+leucovorin+L-OHP (FOLFOX) or capecitabine+L-OHP (XELOX). miR-124 overexpression promoted L-OHP-induced, but not 5-FU-induced, cytotoxicity and apoptosis in HT29 and SW480 cells. CAPN2 was a direct target of miR-124, and its protein expression was reduced by forced expression of miR-124. miR-124 inhibited tumorigenesis and promoted OS of mice bearing xenograft tumors, especially upon L-OHP treatment. miR-124 also promoted L-OHP-induced apoptosis and restrained CAPN2 protein expression in xenograft tumors. Our results suggest that miR-124 could be considered as both a predictor of L-OHP-based chemotherapy for personalized treatment and a therapeutic target for CRC. American Society of Gene & Cell Therapy 2020-04-14 /pmc/articles/PMC7200624/ /pubmed/32382656 http://dx.doi.org/10.1016/j.omto.2020.04.003 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Xie, Xu-Qin Wang, Mo-Jin Li, Yuan Lei, Lin-Ping Wang, Ning Lv, Zhao-Ying Chen, Ke-Ling Zhou, Bin Ping, Jie Zhou, Zong-Guang Sun, Xiao-Feng miR-124 Intensified Oxaliplatin-Based Chemotherapy by Targeting CAPN2 in Colorectal Cancer |
title | miR-124 Intensified Oxaliplatin-Based Chemotherapy by Targeting CAPN2 in Colorectal Cancer |
title_full | miR-124 Intensified Oxaliplatin-Based Chemotherapy by Targeting CAPN2 in Colorectal Cancer |
title_fullStr | miR-124 Intensified Oxaliplatin-Based Chemotherapy by Targeting CAPN2 in Colorectal Cancer |
title_full_unstemmed | miR-124 Intensified Oxaliplatin-Based Chemotherapy by Targeting CAPN2 in Colorectal Cancer |
title_short | miR-124 Intensified Oxaliplatin-Based Chemotherapy by Targeting CAPN2 in Colorectal Cancer |
title_sort | mir-124 intensified oxaliplatin-based chemotherapy by targeting capn2 in colorectal cancer |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7200624/ https://www.ncbi.nlm.nih.gov/pubmed/32382656 http://dx.doi.org/10.1016/j.omto.2020.04.003 |
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