Cargando…

Inorganic polyphosphate is produced and hydrolyzed in F(0)F(1)-ATP synthase of mammalian mitochondria

Inorganic polyphosphate (polyP) is a polymer present in all living organisms. Although polyP is found to be involved in a variety of functions in cells of higher organisms, the enzyme responsible for polyP production and consumption has not yet been identified. Here, we studied the effect of polyP o...

Descripción completa

Detalles Bibliográficos
Autores principales: Baev, Artyom Y., Angelova, Plamena R., Abramov, Andrey Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7200627/
https://www.ncbi.nlm.nih.gov/pubmed/32270854
http://dx.doi.org/10.1042/BCJ20200042
_version_ 1783529374302076928
author Baev, Artyom Y.
Angelova, Plamena R.
Abramov, Andrey Y.
author_facet Baev, Artyom Y.
Angelova, Plamena R.
Abramov, Andrey Y.
author_sort Baev, Artyom Y.
collection PubMed
description Inorganic polyphosphate (polyP) is a polymer present in all living organisms. Although polyP is found to be involved in a variety of functions in cells of higher organisms, the enzyme responsible for polyP production and consumption has not yet been identified. Here, we studied the effect of polyP on mitochondrial respiration, oxidative phosphorylation and activity of F(0)F(1)-ATPsynthase. We have found that polyP activates mitochondrial respiration which does not coupled with ATP production (V(2)) but inhibits ADP-dependent respiration (V(3)). Moreover, PolyP can stimulate F(0)F(1)-ATPase activity in the presence of ATP and, importantly, can be hydrolyzed in this enzyme instead of ATP. Furthermore, PolyP can be produced in mitochondria in the presence of substrates for respiration and phosphate by the F(0)F(1)-ATPsynthase. Thus, polyP is an energy molecule in mammalian cells which can be produced and hydrolyzed in the mitochondrial F(0)F(1)-ATPsynthase.
format Online
Article
Text
id pubmed-7200627
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Portland Press Ltd.
record_format MEDLINE/PubMed
spelling pubmed-72006272020-05-13 Inorganic polyphosphate is produced and hydrolyzed in F(0)F(1)-ATP synthase of mammalian mitochondria Baev, Artyom Y. Angelova, Plamena R. Abramov, Andrey Y. Biochem J Bioenergetics Inorganic polyphosphate (polyP) is a polymer present in all living organisms. Although polyP is found to be involved in a variety of functions in cells of higher organisms, the enzyme responsible for polyP production and consumption has not yet been identified. Here, we studied the effect of polyP on mitochondrial respiration, oxidative phosphorylation and activity of F(0)F(1)-ATPsynthase. We have found that polyP activates mitochondrial respiration which does not coupled with ATP production (V(2)) but inhibits ADP-dependent respiration (V(3)). Moreover, PolyP can stimulate F(0)F(1)-ATPase activity in the presence of ATP and, importantly, can be hydrolyzed in this enzyme instead of ATP. Furthermore, PolyP can be produced in mitochondria in the presence of substrates for respiration and phosphate by the F(0)F(1)-ATPsynthase. Thus, polyP is an energy molecule in mammalian cells which can be produced and hydrolyzed in the mitochondrial F(0)F(1)-ATPsynthase. Portland Press Ltd. 2020-04-30 2020-04-29 /pmc/articles/PMC7200627/ /pubmed/32270854 http://dx.doi.org/10.1042/BCJ20200042 Text en © 2020 The Author(s) https://creativecommons.org/licenses/by/4.0/ This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (https://creativecommons.org/licenses/by/4.0/) . Open access for this article was enabled by the participation of University College London in an all-inclusive Read & Publish pilot with Portland Press and the Biochemical Society under a transformative agreement with JISC.
spellingShingle Bioenergetics
Baev, Artyom Y.
Angelova, Plamena R.
Abramov, Andrey Y.
Inorganic polyphosphate is produced and hydrolyzed in F(0)F(1)-ATP synthase of mammalian mitochondria
title Inorganic polyphosphate is produced and hydrolyzed in F(0)F(1)-ATP synthase of mammalian mitochondria
title_full Inorganic polyphosphate is produced and hydrolyzed in F(0)F(1)-ATP synthase of mammalian mitochondria
title_fullStr Inorganic polyphosphate is produced and hydrolyzed in F(0)F(1)-ATP synthase of mammalian mitochondria
title_full_unstemmed Inorganic polyphosphate is produced and hydrolyzed in F(0)F(1)-ATP synthase of mammalian mitochondria
title_short Inorganic polyphosphate is produced and hydrolyzed in F(0)F(1)-ATP synthase of mammalian mitochondria
title_sort inorganic polyphosphate is produced and hydrolyzed in f(0)f(1)-atp synthase of mammalian mitochondria
topic Bioenergetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7200627/
https://www.ncbi.nlm.nih.gov/pubmed/32270854
http://dx.doi.org/10.1042/BCJ20200042
work_keys_str_mv AT baevartyomy inorganicpolyphosphateisproducedandhydrolyzedinf0f1atpsynthaseofmammalianmitochondria
AT angelovaplamenar inorganicpolyphosphateisproducedandhydrolyzedinf0f1atpsynthaseofmammalianmitochondria
AT abramovandreyy inorganicpolyphosphateisproducedandhydrolyzedinf0f1atpsynthaseofmammalianmitochondria