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Inorganic polyphosphate is produced and hydrolyzed in F(0)F(1)-ATP synthase of mammalian mitochondria
Inorganic polyphosphate (polyP) is a polymer present in all living organisms. Although polyP is found to be involved in a variety of functions in cells of higher organisms, the enzyme responsible for polyP production and consumption has not yet been identified. Here, we studied the effect of polyP o...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7200627/ https://www.ncbi.nlm.nih.gov/pubmed/32270854 http://dx.doi.org/10.1042/BCJ20200042 |
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author | Baev, Artyom Y. Angelova, Plamena R. Abramov, Andrey Y. |
author_facet | Baev, Artyom Y. Angelova, Plamena R. Abramov, Andrey Y. |
author_sort | Baev, Artyom Y. |
collection | PubMed |
description | Inorganic polyphosphate (polyP) is a polymer present in all living organisms. Although polyP is found to be involved in a variety of functions in cells of higher organisms, the enzyme responsible for polyP production and consumption has not yet been identified. Here, we studied the effect of polyP on mitochondrial respiration, oxidative phosphorylation and activity of F(0)F(1)-ATPsynthase. We have found that polyP activates mitochondrial respiration which does not coupled with ATP production (V(2)) but inhibits ADP-dependent respiration (V(3)). Moreover, PolyP can stimulate F(0)F(1)-ATPase activity in the presence of ATP and, importantly, can be hydrolyzed in this enzyme instead of ATP. Furthermore, PolyP can be produced in mitochondria in the presence of substrates for respiration and phosphate by the F(0)F(1)-ATPsynthase. Thus, polyP is an energy molecule in mammalian cells which can be produced and hydrolyzed in the mitochondrial F(0)F(1)-ATPsynthase. |
format | Online Article Text |
id | pubmed-7200627 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-72006272020-05-13 Inorganic polyphosphate is produced and hydrolyzed in F(0)F(1)-ATP synthase of mammalian mitochondria Baev, Artyom Y. Angelova, Plamena R. Abramov, Andrey Y. Biochem J Bioenergetics Inorganic polyphosphate (polyP) is a polymer present in all living organisms. Although polyP is found to be involved in a variety of functions in cells of higher organisms, the enzyme responsible for polyP production and consumption has not yet been identified. Here, we studied the effect of polyP on mitochondrial respiration, oxidative phosphorylation and activity of F(0)F(1)-ATPsynthase. We have found that polyP activates mitochondrial respiration which does not coupled with ATP production (V(2)) but inhibits ADP-dependent respiration (V(3)). Moreover, PolyP can stimulate F(0)F(1)-ATPase activity in the presence of ATP and, importantly, can be hydrolyzed in this enzyme instead of ATP. Furthermore, PolyP can be produced in mitochondria in the presence of substrates for respiration and phosphate by the F(0)F(1)-ATPsynthase. Thus, polyP is an energy molecule in mammalian cells which can be produced and hydrolyzed in the mitochondrial F(0)F(1)-ATPsynthase. Portland Press Ltd. 2020-04-30 2020-04-29 /pmc/articles/PMC7200627/ /pubmed/32270854 http://dx.doi.org/10.1042/BCJ20200042 Text en © 2020 The Author(s) https://creativecommons.org/licenses/by/4.0/ This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (https://creativecommons.org/licenses/by/4.0/) . Open access for this article was enabled by the participation of University College London in an all-inclusive Read & Publish pilot with Portland Press and the Biochemical Society under a transformative agreement with JISC. |
spellingShingle | Bioenergetics Baev, Artyom Y. Angelova, Plamena R. Abramov, Andrey Y. Inorganic polyphosphate is produced and hydrolyzed in F(0)F(1)-ATP synthase of mammalian mitochondria |
title | Inorganic polyphosphate is produced and hydrolyzed in F(0)F(1)-ATP synthase of mammalian mitochondria |
title_full | Inorganic polyphosphate is produced and hydrolyzed in F(0)F(1)-ATP synthase of mammalian mitochondria |
title_fullStr | Inorganic polyphosphate is produced and hydrolyzed in F(0)F(1)-ATP synthase of mammalian mitochondria |
title_full_unstemmed | Inorganic polyphosphate is produced and hydrolyzed in F(0)F(1)-ATP synthase of mammalian mitochondria |
title_short | Inorganic polyphosphate is produced and hydrolyzed in F(0)F(1)-ATP synthase of mammalian mitochondria |
title_sort | inorganic polyphosphate is produced and hydrolyzed in f(0)f(1)-atp synthase of mammalian mitochondria |
topic | Bioenergetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7200627/ https://www.ncbi.nlm.nih.gov/pubmed/32270854 http://dx.doi.org/10.1042/BCJ20200042 |
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