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Development of a defined compost system for the study of plant-microbe interactions

Plant growth promoting rhizobacteria can improve plant health by providing enhanced nutrition, disease suppression and abiotic stress resistance, and have potential to contribute to sustainable agriculture. We have developed a sphagnum peat-based compost platform for investigating plant-microbe inte...

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Autores principales: Masters-Clark, E., Shone, E., Paradelo, M., Hirsch, P. R., Clark, I. M., Otten, W., Brennan, F., Mauchline, T. H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7200721/
https://www.ncbi.nlm.nih.gov/pubmed/32372006
http://dx.doi.org/10.1038/s41598-020-64249-0
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author Masters-Clark, E.
Shone, E.
Paradelo, M.
Hirsch, P. R.
Clark, I. M.
Otten, W.
Brennan, F.
Mauchline, T. H.
author_facet Masters-Clark, E.
Shone, E.
Paradelo, M.
Hirsch, P. R.
Clark, I. M.
Otten, W.
Brennan, F.
Mauchline, T. H.
author_sort Masters-Clark, E.
collection PubMed
description Plant growth promoting rhizobacteria can improve plant health by providing enhanced nutrition, disease suppression and abiotic stress resistance, and have potential to contribute to sustainable agriculture. We have developed a sphagnum peat-based compost platform for investigating plant-microbe interactions. The chemical, physical and biological status of the system can be manipulated to understand the relative importance of these factors for plant health, demonstrated using three case studies: 1. Nutrient depleted compost retained its structure, but plants grown in this medium were severely stunted in growth due to removal of essential soluble nutrients - particularly, nitrogen, phosphorus and potassium. Compost nutrient status was replenished with the addition of selected soluble nutrients, validated by plant biomass; 2. When comparing milled and unmilled compost, we found nutrient status to be more important than matrix structure for plant growth; 3. In compost deficient in soluble P, supplemented with an insoluble inorganic form of P (Ca(3)(PO(4))(2)), application of a phosphate solubilising Pseudomonas strain to plant roots provides a significant growth boost when compared with a Pseudomonas strain incapable of solubilising Ca(3)(PO(4))(2). Our findings show that the compost system can be manipulated to impose biotic and abiotic stresses for testing how microbial inoculants influence plant growth.
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spelling pubmed-72007212020-05-12 Development of a defined compost system for the study of plant-microbe interactions Masters-Clark, E. Shone, E. Paradelo, M. Hirsch, P. R. Clark, I. M. Otten, W. Brennan, F. Mauchline, T. H. Sci Rep Article Plant growth promoting rhizobacteria can improve plant health by providing enhanced nutrition, disease suppression and abiotic stress resistance, and have potential to contribute to sustainable agriculture. We have developed a sphagnum peat-based compost platform for investigating plant-microbe interactions. The chemical, physical and biological status of the system can be manipulated to understand the relative importance of these factors for plant health, demonstrated using three case studies: 1. Nutrient depleted compost retained its structure, but plants grown in this medium were severely stunted in growth due to removal of essential soluble nutrients - particularly, nitrogen, phosphorus and potassium. Compost nutrient status was replenished with the addition of selected soluble nutrients, validated by plant biomass; 2. When comparing milled and unmilled compost, we found nutrient status to be more important than matrix structure for plant growth; 3. In compost deficient in soluble P, supplemented with an insoluble inorganic form of P (Ca(3)(PO(4))(2)), application of a phosphate solubilising Pseudomonas strain to plant roots provides a significant growth boost when compared with a Pseudomonas strain incapable of solubilising Ca(3)(PO(4))(2). Our findings show that the compost system can be manipulated to impose biotic and abiotic stresses for testing how microbial inoculants influence plant growth. Nature Publishing Group UK 2020-05-05 /pmc/articles/PMC7200721/ /pubmed/32372006 http://dx.doi.org/10.1038/s41598-020-64249-0 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Masters-Clark, E.
Shone, E.
Paradelo, M.
Hirsch, P. R.
Clark, I. M.
Otten, W.
Brennan, F.
Mauchline, T. H.
Development of a defined compost system for the study of plant-microbe interactions
title Development of a defined compost system for the study of plant-microbe interactions
title_full Development of a defined compost system for the study of plant-microbe interactions
title_fullStr Development of a defined compost system for the study of plant-microbe interactions
title_full_unstemmed Development of a defined compost system for the study of plant-microbe interactions
title_short Development of a defined compost system for the study of plant-microbe interactions
title_sort development of a defined compost system for the study of plant-microbe interactions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7200721/
https://www.ncbi.nlm.nih.gov/pubmed/32372006
http://dx.doi.org/10.1038/s41598-020-64249-0
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