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Development of a defined compost system for the study of plant-microbe interactions
Plant growth promoting rhizobacteria can improve plant health by providing enhanced nutrition, disease suppression and abiotic stress resistance, and have potential to contribute to sustainable agriculture. We have developed a sphagnum peat-based compost platform for investigating plant-microbe inte...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7200721/ https://www.ncbi.nlm.nih.gov/pubmed/32372006 http://dx.doi.org/10.1038/s41598-020-64249-0 |
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author | Masters-Clark, E. Shone, E. Paradelo, M. Hirsch, P. R. Clark, I. M. Otten, W. Brennan, F. Mauchline, T. H. |
author_facet | Masters-Clark, E. Shone, E. Paradelo, M. Hirsch, P. R. Clark, I. M. Otten, W. Brennan, F. Mauchline, T. H. |
author_sort | Masters-Clark, E. |
collection | PubMed |
description | Plant growth promoting rhizobacteria can improve plant health by providing enhanced nutrition, disease suppression and abiotic stress resistance, and have potential to contribute to sustainable agriculture. We have developed a sphagnum peat-based compost platform for investigating plant-microbe interactions. The chemical, physical and biological status of the system can be manipulated to understand the relative importance of these factors for plant health, demonstrated using three case studies: 1. Nutrient depleted compost retained its structure, but plants grown in this medium were severely stunted in growth due to removal of essential soluble nutrients - particularly, nitrogen, phosphorus and potassium. Compost nutrient status was replenished with the addition of selected soluble nutrients, validated by plant biomass; 2. When comparing milled and unmilled compost, we found nutrient status to be more important than matrix structure for plant growth; 3. In compost deficient in soluble P, supplemented with an insoluble inorganic form of P (Ca(3)(PO(4))(2)), application of a phosphate solubilising Pseudomonas strain to plant roots provides a significant growth boost when compared with a Pseudomonas strain incapable of solubilising Ca(3)(PO(4))(2). Our findings show that the compost system can be manipulated to impose biotic and abiotic stresses for testing how microbial inoculants influence plant growth. |
format | Online Article Text |
id | pubmed-7200721 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-72007212020-05-12 Development of a defined compost system for the study of plant-microbe interactions Masters-Clark, E. Shone, E. Paradelo, M. Hirsch, P. R. Clark, I. M. Otten, W. Brennan, F. Mauchline, T. H. Sci Rep Article Plant growth promoting rhizobacteria can improve plant health by providing enhanced nutrition, disease suppression and abiotic stress resistance, and have potential to contribute to sustainable agriculture. We have developed a sphagnum peat-based compost platform for investigating plant-microbe interactions. The chemical, physical and biological status of the system can be manipulated to understand the relative importance of these factors for plant health, demonstrated using three case studies: 1. Nutrient depleted compost retained its structure, but plants grown in this medium were severely stunted in growth due to removal of essential soluble nutrients - particularly, nitrogen, phosphorus and potassium. Compost nutrient status was replenished with the addition of selected soluble nutrients, validated by plant biomass; 2. When comparing milled and unmilled compost, we found nutrient status to be more important than matrix structure for plant growth; 3. In compost deficient in soluble P, supplemented with an insoluble inorganic form of P (Ca(3)(PO(4))(2)), application of a phosphate solubilising Pseudomonas strain to plant roots provides a significant growth boost when compared with a Pseudomonas strain incapable of solubilising Ca(3)(PO(4))(2). Our findings show that the compost system can be manipulated to impose biotic and abiotic stresses for testing how microbial inoculants influence plant growth. Nature Publishing Group UK 2020-05-05 /pmc/articles/PMC7200721/ /pubmed/32372006 http://dx.doi.org/10.1038/s41598-020-64249-0 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Masters-Clark, E. Shone, E. Paradelo, M. Hirsch, P. R. Clark, I. M. Otten, W. Brennan, F. Mauchline, T. H. Development of a defined compost system for the study of plant-microbe interactions |
title | Development of a defined compost system for the study of plant-microbe interactions |
title_full | Development of a defined compost system for the study of plant-microbe interactions |
title_fullStr | Development of a defined compost system for the study of plant-microbe interactions |
title_full_unstemmed | Development of a defined compost system for the study of plant-microbe interactions |
title_short | Development of a defined compost system for the study of plant-microbe interactions |
title_sort | development of a defined compost system for the study of plant-microbe interactions |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7200721/ https://www.ncbi.nlm.nih.gov/pubmed/32372006 http://dx.doi.org/10.1038/s41598-020-64249-0 |
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