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Enzymatic Cleavage of 3’-Esterified Nucleotides Enables a Long, Continuous DNA Synthesis

The reversible dye-terminator (RDT)-based DNA sequencing-by-synthesis (SBS) chemistry has driven the advancement of the next-generation sequencing technologies for the past two decades. The RDT-based SBS chemistry relies on the DNA polymerase reaction to incorporate the RDT nucleotide (NT) for extra...

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Detalles Bibliográficos
Autores principales:	LinWu, Shiuan-Woei, Tsai, Ting-Yueh, Tu, Yu-Hsuan, Chi, Hung-Wen, Tsao, Yu-Ping, Chen, Ya-Chen, Wang, Hsiang-Ming, Chang, Wei-Hsin, Chiou, Chung-Fan, Lee, Johnsee, Chen, Cheng-Yao
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2020
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7200780/ https://www.ncbi.nlm.nih.gov/pubmed/32372056 http://dx.doi.org/10.1038/s41598-020-64541-z

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