Cargando…
Transcriptome profiling reveals insertional mutagenesis suppressed the expression of candidate pathogenicity genes in honeybee fungal pathogen, Ascosphaera apis
Chalkbrood disease is caused by Ascosphaera apis which severely affects honeybee brood. Spore inoculation experiments shown pathogenicity varies among different strains and mutants, however, the molecular mechanism of pathogenicity is unclear. We sequenced, assembled and annotated the transcriptomes...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7200787/ https://www.ncbi.nlm.nih.gov/pubmed/32372055 http://dx.doi.org/10.1038/s41598-020-64022-3 |
_version_ | 1783529411186786304 |
---|---|
author | Getachew, Awraris Abejew, Tessema Aynalem Wu, Jiangli Xu, Jin Yu, Huimin Tan, Jing Wu, Pengjie Tu, Yangyang Kang, Weipeng Wang, Zheng Xu, Shufa |
author_facet | Getachew, Awraris Abejew, Tessema Aynalem Wu, Jiangli Xu, Jin Yu, Huimin Tan, Jing Wu, Pengjie Tu, Yangyang Kang, Weipeng Wang, Zheng Xu, Shufa |
author_sort | Getachew, Awraris |
collection | PubMed |
description | Chalkbrood disease is caused by Ascosphaera apis which severely affects honeybee brood. Spore inoculation experiments shown pathogenicity varies among different strains and mutants, however, the molecular mechanism of pathogenicity is unclear. We sequenced, assembled and annotated the transcriptomes of wild type (SPE1) and three mutants (SPE2, SPE3 and SPE4) with reduced pathogenicity that were constructed in our previous study. Illumina sequencing generated a total of 394,910,604 clean reads and de novo Trinity-based assembled into 12,989 unigenes, among these, 9,598 genes were successfully annotated to known proteins in UniProt database. A total of 172, 3,996, and 650 genes were up-regulated and 4,403, 2,845, and 3,016 genes were down-regulated between SPE2-SPE1, SPE3-SPE1, and SPE4-SPE1, respectively. Overall, several genes with a potential role in fungal pathogenicity were detected down-regulated in mutants including 100 hydrolytic enzymes, 117 transcriptional factors, and 47 cell wall related genes. KEGG pathway enrichment analysis reveals 216 genes involved in nine pathways were down-regulated in mutants compared to wild type. The down-regulation of more pathways involved in pathogenicity in SPE2 and SPE4 than SPE3 supports their lower pathogenicity during in-vitro bioassay experiment. Expression of 12 down-regulated genes in mutants was validated by quantitative real time PCR. This study provides valuable information on transcriptome variation caused by mutation for further functional validation of candidate pathogenicity genes in A. apis. |
format | Online Article Text |
id | pubmed-7200787 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-72007872020-05-12 Transcriptome profiling reveals insertional mutagenesis suppressed the expression of candidate pathogenicity genes in honeybee fungal pathogen, Ascosphaera apis Getachew, Awraris Abejew, Tessema Aynalem Wu, Jiangli Xu, Jin Yu, Huimin Tan, Jing Wu, Pengjie Tu, Yangyang Kang, Weipeng Wang, Zheng Xu, Shufa Sci Rep Article Chalkbrood disease is caused by Ascosphaera apis which severely affects honeybee brood. Spore inoculation experiments shown pathogenicity varies among different strains and mutants, however, the molecular mechanism of pathogenicity is unclear. We sequenced, assembled and annotated the transcriptomes of wild type (SPE1) and three mutants (SPE2, SPE3 and SPE4) with reduced pathogenicity that were constructed in our previous study. Illumina sequencing generated a total of 394,910,604 clean reads and de novo Trinity-based assembled into 12,989 unigenes, among these, 9,598 genes were successfully annotated to known proteins in UniProt database. A total of 172, 3,996, and 650 genes were up-regulated and 4,403, 2,845, and 3,016 genes were down-regulated between SPE2-SPE1, SPE3-SPE1, and SPE4-SPE1, respectively. Overall, several genes with a potential role in fungal pathogenicity were detected down-regulated in mutants including 100 hydrolytic enzymes, 117 transcriptional factors, and 47 cell wall related genes. KEGG pathway enrichment analysis reveals 216 genes involved in nine pathways were down-regulated in mutants compared to wild type. The down-regulation of more pathways involved in pathogenicity in SPE2 and SPE4 than SPE3 supports their lower pathogenicity during in-vitro bioassay experiment. Expression of 12 down-regulated genes in mutants was validated by quantitative real time PCR. This study provides valuable information on transcriptome variation caused by mutation for further functional validation of candidate pathogenicity genes in A. apis. Nature Publishing Group UK 2020-05-05 /pmc/articles/PMC7200787/ /pubmed/32372055 http://dx.doi.org/10.1038/s41598-020-64022-3 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Getachew, Awraris Abejew, Tessema Aynalem Wu, Jiangli Xu, Jin Yu, Huimin Tan, Jing Wu, Pengjie Tu, Yangyang Kang, Weipeng Wang, Zheng Xu, Shufa Transcriptome profiling reveals insertional mutagenesis suppressed the expression of candidate pathogenicity genes in honeybee fungal pathogen, Ascosphaera apis |
title | Transcriptome profiling reveals insertional mutagenesis suppressed the expression of candidate pathogenicity genes in honeybee fungal pathogen, Ascosphaera apis |
title_full | Transcriptome profiling reveals insertional mutagenesis suppressed the expression of candidate pathogenicity genes in honeybee fungal pathogen, Ascosphaera apis |
title_fullStr | Transcriptome profiling reveals insertional mutagenesis suppressed the expression of candidate pathogenicity genes in honeybee fungal pathogen, Ascosphaera apis |
title_full_unstemmed | Transcriptome profiling reveals insertional mutagenesis suppressed the expression of candidate pathogenicity genes in honeybee fungal pathogen, Ascosphaera apis |
title_short | Transcriptome profiling reveals insertional mutagenesis suppressed the expression of candidate pathogenicity genes in honeybee fungal pathogen, Ascosphaera apis |
title_sort | transcriptome profiling reveals insertional mutagenesis suppressed the expression of candidate pathogenicity genes in honeybee fungal pathogen, ascosphaera apis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7200787/ https://www.ncbi.nlm.nih.gov/pubmed/32372055 http://dx.doi.org/10.1038/s41598-020-64022-3 |
work_keys_str_mv | AT getachewawraris transcriptomeprofilingrevealsinsertionalmutagenesissuppressedtheexpressionofcandidatepathogenicitygenesinhoneybeefungalpathogenascosphaeraapis AT abejewtessemaaynalem transcriptomeprofilingrevealsinsertionalmutagenesissuppressedtheexpressionofcandidatepathogenicitygenesinhoneybeefungalpathogenascosphaeraapis AT wujiangli transcriptomeprofilingrevealsinsertionalmutagenesissuppressedtheexpressionofcandidatepathogenicitygenesinhoneybeefungalpathogenascosphaeraapis AT xujin transcriptomeprofilingrevealsinsertionalmutagenesissuppressedtheexpressionofcandidatepathogenicitygenesinhoneybeefungalpathogenascosphaeraapis AT yuhuimin transcriptomeprofilingrevealsinsertionalmutagenesissuppressedtheexpressionofcandidatepathogenicitygenesinhoneybeefungalpathogenascosphaeraapis AT tanjing transcriptomeprofilingrevealsinsertionalmutagenesissuppressedtheexpressionofcandidatepathogenicitygenesinhoneybeefungalpathogenascosphaeraapis AT wupengjie transcriptomeprofilingrevealsinsertionalmutagenesissuppressedtheexpressionofcandidatepathogenicitygenesinhoneybeefungalpathogenascosphaeraapis AT tuyangyang transcriptomeprofilingrevealsinsertionalmutagenesissuppressedtheexpressionofcandidatepathogenicitygenesinhoneybeefungalpathogenascosphaeraapis AT kangweipeng transcriptomeprofilingrevealsinsertionalmutagenesissuppressedtheexpressionofcandidatepathogenicitygenesinhoneybeefungalpathogenascosphaeraapis AT wangzheng transcriptomeprofilingrevealsinsertionalmutagenesissuppressedtheexpressionofcandidatepathogenicitygenesinhoneybeefungalpathogenascosphaeraapis AT xushufa transcriptomeprofilingrevealsinsertionalmutagenesissuppressedtheexpressionofcandidatepathogenicitygenesinhoneybeefungalpathogenascosphaeraapis |