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scFv against HSP60 of Strongyloides sp. and Its Application in the Evaluation of Parasite Frequency in the Elderly
The present study is aimed at evaluating serological method using scFv anti-Strongyloides sp. and reporting the frequencies of the results with conventional parasitological technique (faeces) in elderly individuals. Among 112 elderly individuals (≥60 years of age), 14.28% were positive for at least...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7201452/ https://www.ncbi.nlm.nih.gov/pubmed/32399087 http://dx.doi.org/10.1155/2020/4086929 |
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author | Miguel, Camila Botelho Levenhagen, Marcelo Arantes Costa-Cruz, Julia Maria Goulart, Luiz Ricardo Alves, Patrícia Terra Ueira-Vieira, Carlos Brito, Patrícia Kellen Martins Oliveira Gomes, Angelica Oliveira Lazo-Chica, Javier Emilio Oliveira, Carlo José Freire Rodrigues, Wellington Francisco |
author_facet | Miguel, Camila Botelho Levenhagen, Marcelo Arantes Costa-Cruz, Julia Maria Goulart, Luiz Ricardo Alves, Patrícia Terra Ueira-Vieira, Carlos Brito, Patrícia Kellen Martins Oliveira Gomes, Angelica Oliveira Lazo-Chica, Javier Emilio Oliveira, Carlo José Freire Rodrigues, Wellington Francisco |
author_sort | Miguel, Camila Botelho |
collection | PubMed |
description | The present study is aimed at evaluating serological method using scFv anti-Strongyloides sp. and reporting the frequencies of the results with conventional parasitological technique (faeces) in elderly individuals. Among 112 elderly individuals (≥60 years of age), 14.28% were positive for at least one enteroparasite, with one individual positive for S. stercoralis. Sera were evaluated for the presence of anti-Strongyloides sp. antibodies using total or detergent fraction extracts of Strongyloides venezuelensis, which presented positivity rates of 19.64% and 10.71%, respectively. An anti-HSP60 single-chain variable fragment from Strongyloides sp. was used to detect parasite antigens, with 5.36% (6 individuals) of ELISA-positive individuals returning a positive result. While the serological test indicates previous or recent infection and may be limited by antigen purification, the anti-HSP60 method reflects the presence of Strongyloides sp. immune complexes and exhibits greater sensitivity and specificity. Our results demonstrate the variable occurrence of enteroparasites in elderly individuals residing in long-term nursing homes and validate a novel epidemiological tool to describe infection cases by Strongyloides sp. |
format | Online Article Text |
id | pubmed-7201452 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-72014522020-05-12 scFv against HSP60 of Strongyloides sp. and Its Application in the Evaluation of Parasite Frequency in the Elderly Miguel, Camila Botelho Levenhagen, Marcelo Arantes Costa-Cruz, Julia Maria Goulart, Luiz Ricardo Alves, Patrícia Terra Ueira-Vieira, Carlos Brito, Patrícia Kellen Martins Oliveira Gomes, Angelica Oliveira Lazo-Chica, Javier Emilio Oliveira, Carlo José Freire Rodrigues, Wellington Francisco Dis Markers Research Article The present study is aimed at evaluating serological method using scFv anti-Strongyloides sp. and reporting the frequencies of the results with conventional parasitological technique (faeces) in elderly individuals. Among 112 elderly individuals (≥60 years of age), 14.28% were positive for at least one enteroparasite, with one individual positive for S. stercoralis. Sera were evaluated for the presence of anti-Strongyloides sp. antibodies using total or detergent fraction extracts of Strongyloides venezuelensis, which presented positivity rates of 19.64% and 10.71%, respectively. An anti-HSP60 single-chain variable fragment from Strongyloides sp. was used to detect parasite antigens, with 5.36% (6 individuals) of ELISA-positive individuals returning a positive result. While the serological test indicates previous or recent infection and may be limited by antigen purification, the anti-HSP60 method reflects the presence of Strongyloides sp. immune complexes and exhibits greater sensitivity and specificity. Our results demonstrate the variable occurrence of enteroparasites in elderly individuals residing in long-term nursing homes and validate a novel epidemiological tool to describe infection cases by Strongyloides sp. Hindawi 2020-01-11 /pmc/articles/PMC7201452/ /pubmed/32399087 http://dx.doi.org/10.1155/2020/4086929 Text en Copyright © 2020 Camila Botelho Miguel et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Miguel, Camila Botelho Levenhagen, Marcelo Arantes Costa-Cruz, Julia Maria Goulart, Luiz Ricardo Alves, Patrícia Terra Ueira-Vieira, Carlos Brito, Patrícia Kellen Martins Oliveira Gomes, Angelica Oliveira Lazo-Chica, Javier Emilio Oliveira, Carlo José Freire Rodrigues, Wellington Francisco scFv against HSP60 of Strongyloides sp. and Its Application in the Evaluation of Parasite Frequency in the Elderly |
title | scFv against HSP60 of Strongyloides sp. and Its Application in the Evaluation of Parasite Frequency in the Elderly |
title_full | scFv against HSP60 of Strongyloides sp. and Its Application in the Evaluation of Parasite Frequency in the Elderly |
title_fullStr | scFv against HSP60 of Strongyloides sp. and Its Application in the Evaluation of Parasite Frequency in the Elderly |
title_full_unstemmed | scFv against HSP60 of Strongyloides sp. and Its Application in the Evaluation of Parasite Frequency in the Elderly |
title_short | scFv against HSP60 of Strongyloides sp. and Its Application in the Evaluation of Parasite Frequency in the Elderly |
title_sort | scfv against hsp60 of strongyloides sp. and its application in the evaluation of parasite frequency in the elderly |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7201452/ https://www.ncbi.nlm.nih.gov/pubmed/32399087 http://dx.doi.org/10.1155/2020/4086929 |
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