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Anti-Inflammatory Effects of Modified Buyang Huanwu Decoction
METHODS: A cytotoxicity assay for BHD was performed using the MTT assay. Following treatment with BHD, mBHD-1, and mBHD-2 in the presence of lipopolysaccharide (LPS), nitric oxide (NO) secretion was detected in cell supernatants using a NO detection kit. The expression of proinflammatory mediators w...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7201499/ https://www.ncbi.nlm.nih.gov/pubmed/32419816 http://dx.doi.org/10.1155/2020/6458460 |
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author | Ryu, Hyang-Hwa Kang, Jun-Chul Namgung, Uk Kim, Song-Yi Park, Ji-Yeun |
author_facet | Ryu, Hyang-Hwa Kang, Jun-Chul Namgung, Uk Kim, Song-Yi Park, Ji-Yeun |
author_sort | Ryu, Hyang-Hwa |
collection | PubMed |
description | METHODS: A cytotoxicity assay for BHD was performed using the MTT assay. Following treatment with BHD, mBHD-1, and mBHD-2 in the presence of lipopolysaccharide (LPS), nitric oxide (NO) secretion was detected in cell supernatants using a NO detection kit. The expression of proinflammatory mediators was detected using RT-PCR and western blotting. To verify the mechanism of mBHD, specific inhibitors of JNK (SP600125) or p38 (SB203580) were used for co-treatment with mBHD, and then the changes in NO and nitric oxide synthase (iNOS) were measured. RESULTS: Both mBHD-1 and mBHD-2 showed greater anti-inflammatory effects than BHD. Both mBHD-1 and mBHD-2 inhibited NO secretion and decreased the expression of IL-1β, IL-6, TNF-α, and iNOS. Treatment with a p38 inhibitor and a JNK inhibitor in mBHD-1- and mBHD-2-treated cells resulted in inhibition of NO and iNOS. CONCLUSION: We provided the first experimental evidence that mBHD may be a more useful anti-inflammatory than BHD. High concentrations or long-term use of BHD may be harmful to inflammatory status. Therefore, the length of treatment and concentration should be considered depending on the targeted disease. |
format | Online Article Text |
id | pubmed-7201499 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-72014992020-05-15 Anti-Inflammatory Effects of Modified Buyang Huanwu Decoction Ryu, Hyang-Hwa Kang, Jun-Chul Namgung, Uk Kim, Song-Yi Park, Ji-Yeun Evid Based Complement Alternat Med Research Article METHODS: A cytotoxicity assay for BHD was performed using the MTT assay. Following treatment with BHD, mBHD-1, and mBHD-2 in the presence of lipopolysaccharide (LPS), nitric oxide (NO) secretion was detected in cell supernatants using a NO detection kit. The expression of proinflammatory mediators was detected using RT-PCR and western blotting. To verify the mechanism of mBHD, specific inhibitors of JNK (SP600125) or p38 (SB203580) were used for co-treatment with mBHD, and then the changes in NO and nitric oxide synthase (iNOS) were measured. RESULTS: Both mBHD-1 and mBHD-2 showed greater anti-inflammatory effects than BHD. Both mBHD-1 and mBHD-2 inhibited NO secretion and decreased the expression of IL-1β, IL-6, TNF-α, and iNOS. Treatment with a p38 inhibitor and a JNK inhibitor in mBHD-1- and mBHD-2-treated cells resulted in inhibition of NO and iNOS. CONCLUSION: We provided the first experimental evidence that mBHD may be a more useful anti-inflammatory than BHD. High concentrations or long-term use of BHD may be harmful to inflammatory status. Therefore, the length of treatment and concentration should be considered depending on the targeted disease. Hindawi 2020-01-13 /pmc/articles/PMC7201499/ /pubmed/32419816 http://dx.doi.org/10.1155/2020/6458460 Text en Copyright © 2020 Hyang-Hwa Ryu et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Ryu, Hyang-Hwa Kang, Jun-Chul Namgung, Uk Kim, Song-Yi Park, Ji-Yeun Anti-Inflammatory Effects of Modified Buyang Huanwu Decoction |
title | Anti-Inflammatory Effects of Modified Buyang Huanwu Decoction |
title_full | Anti-Inflammatory Effects of Modified Buyang Huanwu Decoction |
title_fullStr | Anti-Inflammatory Effects of Modified Buyang Huanwu Decoction |
title_full_unstemmed | Anti-Inflammatory Effects of Modified Buyang Huanwu Decoction |
title_short | Anti-Inflammatory Effects of Modified Buyang Huanwu Decoction |
title_sort | anti-inflammatory effects of modified buyang huanwu decoction |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7201499/ https://www.ncbi.nlm.nih.gov/pubmed/32419816 http://dx.doi.org/10.1155/2020/6458460 |
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