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Distinguishing Kawasaki Disease from Febrile Infectious Disease Using Gene Pair Signatures
Kawasaki disease (KD) is an acute systemic vasculitis of childhood with prolonged fever, and the diagnosis of KD is mainly based on clinical criteria, which is prone to misdiagnosis with other febrile infectious (FI) diseases. Currently, there remain no effective molecular markers for KD diagnosis....
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7201505/ https://www.ncbi.nlm.nih.gov/pubmed/32420360 http://dx.doi.org/10.1155/2020/6539398 |
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author | Zhong, Jiayong Huang, Qingsheng Wang, Yanfei Gao, Huan Jia, Hongling Fan, Jun Liang, Huiying |
author_facet | Zhong, Jiayong Huang, Qingsheng Wang, Yanfei Gao, Huan Jia, Hongling Fan, Jun Liang, Huiying |
author_sort | Zhong, Jiayong |
collection | PubMed |
description | Kawasaki disease (KD) is an acute systemic vasculitis of childhood with prolonged fever, and the diagnosis of KD is mainly based on clinical criteria, which is prone to misdiagnosis with other febrile infectious (FI) diseases. Currently, there remain no effective molecular markers for KD diagnosis. In this study, we aimed to use a relative-expression-based method k-TSP and resampling framework to identify robust gene pair signatures to distinguish KD from bacterial and virus febrile infectious diseases. Our study pool consisted of 808 childhood patients from several studies and assigned to three groups, namely, the discovery set (n = 224), validation set-1 (n = 197), and validation set-2 (n = 387). We had identified 60 biologically relevant gene pairs and developed a top-ranked gene pair classifier (TRGP) using the first seven signatures, with the area under the receiver-operating characteristic curves (AUROC) of 0.947 (95% CI, 0.918-0.976), a sensitivity of 0.936 (95% CI, 0.872-0.987), and a specificity of 0.774 (95% CI, 0.705-0.836) in the discovery set. In the validation set-1, the TRGP classifier distinguished KD from FI with AUROC of 0.955 (95% CI, 0.919-0.991), a sensitivity of 0.959 (95% CI, 0.925-0.986), and a specificity of 0.863 (95% CI, 0.764-0.961). In the validation set-2, the predictive performance of classification was with an AUROC of 0.796 (95% CI, 0.747-0.845), a sensitivity of 0.797 (95% CI, 0.720-0.864), and a specificity of 0.661 (95% CI, 0.606-0.717). Our study reveals that gene pair signatures are robust across diverse studies and can be utilized as objective biomarkers to distinguish KD from FI, helping to develop a fast, simple, and effective molecular approach to improve the diagnosis of KD. |
format | Online Article Text |
id | pubmed-7201505 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-72015052020-05-15 Distinguishing Kawasaki Disease from Febrile Infectious Disease Using Gene Pair Signatures Zhong, Jiayong Huang, Qingsheng Wang, Yanfei Gao, Huan Jia, Hongling Fan, Jun Liang, Huiying Biomed Res Int Research Article Kawasaki disease (KD) is an acute systemic vasculitis of childhood with prolonged fever, and the diagnosis of KD is mainly based on clinical criteria, which is prone to misdiagnosis with other febrile infectious (FI) diseases. Currently, there remain no effective molecular markers for KD diagnosis. In this study, we aimed to use a relative-expression-based method k-TSP and resampling framework to identify robust gene pair signatures to distinguish KD from bacterial and virus febrile infectious diseases. Our study pool consisted of 808 childhood patients from several studies and assigned to three groups, namely, the discovery set (n = 224), validation set-1 (n = 197), and validation set-2 (n = 387). We had identified 60 biologically relevant gene pairs and developed a top-ranked gene pair classifier (TRGP) using the first seven signatures, with the area under the receiver-operating characteristic curves (AUROC) of 0.947 (95% CI, 0.918-0.976), a sensitivity of 0.936 (95% CI, 0.872-0.987), and a specificity of 0.774 (95% CI, 0.705-0.836) in the discovery set. In the validation set-1, the TRGP classifier distinguished KD from FI with AUROC of 0.955 (95% CI, 0.919-0.991), a sensitivity of 0.959 (95% CI, 0.925-0.986), and a specificity of 0.863 (95% CI, 0.764-0.961). In the validation set-2, the predictive performance of classification was with an AUROC of 0.796 (95% CI, 0.747-0.845), a sensitivity of 0.797 (95% CI, 0.720-0.864), and a specificity of 0.661 (95% CI, 0.606-0.717). Our study reveals that gene pair signatures are robust across diverse studies and can be utilized as objective biomarkers to distinguish KD from FI, helping to develop a fast, simple, and effective molecular approach to improve the diagnosis of KD. Hindawi 2020-04-26 /pmc/articles/PMC7201505/ /pubmed/32420360 http://dx.doi.org/10.1155/2020/6539398 Text en Copyright © 2020 Jiayong Zhong et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Zhong, Jiayong Huang, Qingsheng Wang, Yanfei Gao, Huan Jia, Hongling Fan, Jun Liang, Huiying Distinguishing Kawasaki Disease from Febrile Infectious Disease Using Gene Pair Signatures |
title | Distinguishing Kawasaki Disease from Febrile Infectious Disease Using Gene Pair Signatures |
title_full | Distinguishing Kawasaki Disease from Febrile Infectious Disease Using Gene Pair Signatures |
title_fullStr | Distinguishing Kawasaki Disease from Febrile Infectious Disease Using Gene Pair Signatures |
title_full_unstemmed | Distinguishing Kawasaki Disease from Febrile Infectious Disease Using Gene Pair Signatures |
title_short | Distinguishing Kawasaki Disease from Febrile Infectious Disease Using Gene Pair Signatures |
title_sort | distinguishing kawasaki disease from febrile infectious disease using gene pair signatures |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7201505/ https://www.ncbi.nlm.nih.gov/pubmed/32420360 http://dx.doi.org/10.1155/2020/6539398 |
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