Cargando…

Species-Specific Duplication Event Associated with Elevated Levels of Nonstructural Carbohydrates in Sorghum bicolor

Simple sugars are the essential foundation to plant life, and thus, their production, utilization, and storage are highly regulated processes with many complex genetic controls. Despite their importance, many of the genetic and biochemical mechanisms remain unknown or uncharacterized. Sorghum, a hig...

Descripción completa

Detalles Bibliográficos
Autores principales: Brenton, Zachary W., Juengst, Brendon T., Cooper, Elizabeth A., Myers, Matthew T., Jordan, Kathleen E., Dale, Savanah M., Glaubitz, Jeffrey C., Wang, Xiaoyun, Boyles, Richard E., Connolly, Erin L., Kresovich, Stephen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7202026/
https://www.ncbi.nlm.nih.gov/pubmed/32132167
http://dx.doi.org/10.1534/g3.119.400921
_version_ 1783529658338246656
author Brenton, Zachary W.
Juengst, Brendon T.
Cooper, Elizabeth A.
Myers, Matthew T.
Jordan, Kathleen E.
Dale, Savanah M.
Glaubitz, Jeffrey C.
Wang, Xiaoyun
Boyles, Richard E.
Connolly, Erin L.
Kresovich, Stephen
author_facet Brenton, Zachary W.
Juengst, Brendon T.
Cooper, Elizabeth A.
Myers, Matthew T.
Jordan, Kathleen E.
Dale, Savanah M.
Glaubitz, Jeffrey C.
Wang, Xiaoyun
Boyles, Richard E.
Connolly, Erin L.
Kresovich, Stephen
author_sort Brenton, Zachary W.
collection PubMed
description Simple sugars are the essential foundation to plant life, and thus, their production, utilization, and storage are highly regulated processes with many complex genetic controls. Despite their importance, many of the genetic and biochemical mechanisms remain unknown or uncharacterized. Sorghum, a highly productive, diverse C(4) grass important for both industrial and subsistence agricultural systems, has considerable phenotypic diversity in the accumulation of nonstructural sugars in the stem. We use this crop species to examine the genetic controls of high levels of sugar accumulation, identify genetic mechanisms for the accumulation of nonstructural sugars, and link carbon allocation with iron transport. We identify a species-specific tandem duplication event controlling sugar accumulation using genome-wide association analysis, characterize multiple allelic variants causing increased sugar content, and provide further evidence of a putative neofunctionalization event conferring adaptability in Sorghum bicolor. Comparative genomics indicate that this event is unique to sorghum which may further elucidate evolutionary mechanisms for adaptation and divergence within the Poaceae. Furthermore, the identification and characterization of this event was only possible with the continued advancement and improvement of the reference genome. The characterization of this region and the process in which it was discovered serve as a reminder that any reference genome is imperfect and is in need of continual improvement.
format Online
Article
Text
id pubmed-7202026
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Genetics Society of America
record_format MEDLINE/PubMed
spelling pubmed-72020262020-05-09 Species-Specific Duplication Event Associated with Elevated Levels of Nonstructural Carbohydrates in Sorghum bicolor Brenton, Zachary W. Juengst, Brendon T. Cooper, Elizabeth A. Myers, Matthew T. Jordan, Kathleen E. Dale, Savanah M. Glaubitz, Jeffrey C. Wang, Xiaoyun Boyles, Richard E. Connolly, Erin L. Kresovich, Stephen G3 (Bethesda) Investigations Simple sugars are the essential foundation to plant life, and thus, their production, utilization, and storage are highly regulated processes with many complex genetic controls. Despite their importance, many of the genetic and biochemical mechanisms remain unknown or uncharacterized. Sorghum, a highly productive, diverse C(4) grass important for both industrial and subsistence agricultural systems, has considerable phenotypic diversity in the accumulation of nonstructural sugars in the stem. We use this crop species to examine the genetic controls of high levels of sugar accumulation, identify genetic mechanisms for the accumulation of nonstructural sugars, and link carbon allocation with iron transport. We identify a species-specific tandem duplication event controlling sugar accumulation using genome-wide association analysis, characterize multiple allelic variants causing increased sugar content, and provide further evidence of a putative neofunctionalization event conferring adaptability in Sorghum bicolor. Comparative genomics indicate that this event is unique to sorghum which may further elucidate evolutionary mechanisms for adaptation and divergence within the Poaceae. Furthermore, the identification and characterization of this event was only possible with the continued advancement and improvement of the reference genome. The characterization of this region and the process in which it was discovered serve as a reminder that any reference genome is imperfect and is in need of continual improvement. Genetics Society of America 2020-03-04 /pmc/articles/PMC7202026/ /pubmed/32132167 http://dx.doi.org/10.1534/g3.119.400921 Text en Copyright © 2020 Brenton et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Investigations
Brenton, Zachary W.
Juengst, Brendon T.
Cooper, Elizabeth A.
Myers, Matthew T.
Jordan, Kathleen E.
Dale, Savanah M.
Glaubitz, Jeffrey C.
Wang, Xiaoyun
Boyles, Richard E.
Connolly, Erin L.
Kresovich, Stephen
Species-Specific Duplication Event Associated with Elevated Levels of Nonstructural Carbohydrates in Sorghum bicolor
title Species-Specific Duplication Event Associated with Elevated Levels of Nonstructural Carbohydrates in Sorghum bicolor
title_full Species-Specific Duplication Event Associated with Elevated Levels of Nonstructural Carbohydrates in Sorghum bicolor
title_fullStr Species-Specific Duplication Event Associated with Elevated Levels of Nonstructural Carbohydrates in Sorghum bicolor
title_full_unstemmed Species-Specific Duplication Event Associated with Elevated Levels of Nonstructural Carbohydrates in Sorghum bicolor
title_short Species-Specific Duplication Event Associated with Elevated Levels of Nonstructural Carbohydrates in Sorghum bicolor
title_sort species-specific duplication event associated with elevated levels of nonstructural carbohydrates in sorghum bicolor
topic Investigations
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7202026/
https://www.ncbi.nlm.nih.gov/pubmed/32132167
http://dx.doi.org/10.1534/g3.119.400921
work_keys_str_mv AT brentonzacharyw speciesspecificduplicationeventassociatedwithelevatedlevelsofnonstructuralcarbohydratesinsorghumbicolor
AT juengstbrendont speciesspecificduplicationeventassociatedwithelevatedlevelsofnonstructuralcarbohydratesinsorghumbicolor
AT cooperelizabetha speciesspecificduplicationeventassociatedwithelevatedlevelsofnonstructuralcarbohydratesinsorghumbicolor
AT myersmatthewt speciesspecificduplicationeventassociatedwithelevatedlevelsofnonstructuralcarbohydratesinsorghumbicolor
AT jordankathleene speciesspecificduplicationeventassociatedwithelevatedlevelsofnonstructuralcarbohydratesinsorghumbicolor
AT dalesavanahm speciesspecificduplicationeventassociatedwithelevatedlevelsofnonstructuralcarbohydratesinsorghumbicolor
AT glaubitzjeffreyc speciesspecificduplicationeventassociatedwithelevatedlevelsofnonstructuralcarbohydratesinsorghumbicolor
AT wangxiaoyun speciesspecificduplicationeventassociatedwithelevatedlevelsofnonstructuralcarbohydratesinsorghumbicolor
AT boylesricharde speciesspecificduplicationeventassociatedwithelevatedlevelsofnonstructuralcarbohydratesinsorghumbicolor
AT connollyerinl speciesspecificduplicationeventassociatedwithelevatedlevelsofnonstructuralcarbohydratesinsorghumbicolor
AT kresovichstephen speciesspecificduplicationeventassociatedwithelevatedlevelsofnonstructuralcarbohydratesinsorghumbicolor