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Functional characterization of MCAK/Kif2C cancer mutations using high-throughput microscopic analysis

The microtubule (MT)-depolymerizing activity of MCAK/Kif2C can be quantified by expressing the motor in cultured cells and measuring tubulin fluorescence levels after enough hours have passed to allow tubulin autoregulation to proceed. This method allows us to score the impact of point mutations wit...

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Detalles Bibliográficos
Autores principales:	Wagenbach, Mike, Vicente, Juan Jesus, Ovechkina, Yulia, Domnitz, Sarah, Wordeman, Linda
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	The American Society for Cell Biology 2020
Materias:	Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7202071/ https://www.ncbi.nlm.nih.gov/pubmed/31746663 http://dx.doi.org/10.1091/mbc.E19-09-0503

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