Cargando…

Inhibition of miR-9 decreases osteosarcoma cell proliferation

Osteosarcoma (OS) is the most common primary bone tumor that affects adolescents and young adults. Disruption of microRNA (miRNA) regulation is well established in the pathophysiology of different cancers, including OS. Increased expression of miR-9 in OS positively correlates with the tumor size, c...

Descripción completa

Detalles Bibliográficos
Autores principales: Gang, Wu, Tanjun, Wei, Yong, Huang, Jiajun, Qin, Yi, Zhang, Hao, Hu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7202196/
https://www.ncbi.nlm.nih.gov/pubmed/31724522
http://dx.doi.org/10.17305/bjbms.2019.4434
_version_ 1783529674212638720
author Gang, Wu
Tanjun, Wei
Yong, Huang
Jiajun, Qin
Yi, Zhang
Hao, Hu
author_facet Gang, Wu
Tanjun, Wei
Yong, Huang
Jiajun, Qin
Yi, Zhang
Hao, Hu
author_sort Gang, Wu
collection PubMed
description Osteosarcoma (OS) is the most common primary bone tumor that affects adolescents and young adults. Disruption of microRNA (miRNA) regulation is well established in the pathophysiology of different cancers, including OS. Increased expression of miR-9 in OS positively correlates with the tumor size, clinical stage, and distant metastasis. In the present study, we used two different OS cell lines, MG-63 and Saos-2, as in vitro models. miR-9 inhibitor and miR-9 mimics were used to study the function of miR-9 in these two cell lines. We determined the effect of miR-9 inhibition on cell proliferation, cell cycle, apoptosis, and the protein expression of different genes. Our results demonstrated that miR-9 inhibition in the human OS cell lines suppresses their metastatic potential, as determined by decreased cell proliferation and cell cycle arrest, decreased invasion, and increased apoptosis. The Western blot analysis showed that E-cadherin, matrix metalloproteinase 13, forkhead box O3, Bcl-2-like protein 11, and β-catenin are involved in miR-9 signaling. Moreover, miR-9 mimics rescued the effects caused by the inhibition of miR-9 in the OS cell lines. Our findings suggest that miR-9 is important for mediating OS cell migration, invasion, metastasis, and apoptosis. Inhibition of miR-9 could be further explored as a therapeutic target to treat OS.
format Online
Article
Text
id pubmed-7202196
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina
record_format MEDLINE/PubMed
spelling pubmed-72021962020-05-06 Inhibition of miR-9 decreases osteosarcoma cell proliferation Gang, Wu Tanjun, Wei Yong, Huang Jiajun, Qin Yi, Zhang Hao, Hu Bosn J Basic Med Sci Research Article Osteosarcoma (OS) is the most common primary bone tumor that affects adolescents and young adults. Disruption of microRNA (miRNA) regulation is well established in the pathophysiology of different cancers, including OS. Increased expression of miR-9 in OS positively correlates with the tumor size, clinical stage, and distant metastasis. In the present study, we used two different OS cell lines, MG-63 and Saos-2, as in vitro models. miR-9 inhibitor and miR-9 mimics were used to study the function of miR-9 in these two cell lines. We determined the effect of miR-9 inhibition on cell proliferation, cell cycle, apoptosis, and the protein expression of different genes. Our results demonstrated that miR-9 inhibition in the human OS cell lines suppresses their metastatic potential, as determined by decreased cell proliferation and cell cycle arrest, decreased invasion, and increased apoptosis. The Western blot analysis showed that E-cadherin, matrix metalloproteinase 13, forkhead box O3, Bcl-2-like protein 11, and β-catenin are involved in miR-9 signaling. Moreover, miR-9 mimics rescued the effects caused by the inhibition of miR-9 in the OS cell lines. Our findings suggest that miR-9 is important for mediating OS cell migration, invasion, metastasis, and apoptosis. Inhibition of miR-9 could be further explored as a therapeutic target to treat OS. Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina 2020-05 /pmc/articles/PMC7202196/ /pubmed/31724522 http://dx.doi.org/10.17305/bjbms.2019.4434 Text en Copyright: © The Author(s) (2020) http://creativecommons.org/licenses/by-nc-sa/4.0 This work is licensed under a Creative Commons Attribution 4.0 International License
spellingShingle Research Article
Gang, Wu
Tanjun, Wei
Yong, Huang
Jiajun, Qin
Yi, Zhang
Hao, Hu
Inhibition of miR-9 decreases osteosarcoma cell proliferation
title Inhibition of miR-9 decreases osteosarcoma cell proliferation
title_full Inhibition of miR-9 decreases osteosarcoma cell proliferation
title_fullStr Inhibition of miR-9 decreases osteosarcoma cell proliferation
title_full_unstemmed Inhibition of miR-9 decreases osteosarcoma cell proliferation
title_short Inhibition of miR-9 decreases osteosarcoma cell proliferation
title_sort inhibition of mir-9 decreases osteosarcoma cell proliferation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7202196/
https://www.ncbi.nlm.nih.gov/pubmed/31724522
http://dx.doi.org/10.17305/bjbms.2019.4434
work_keys_str_mv AT gangwu inhibitionofmir9decreasesosteosarcomacellproliferation
AT tanjunwei inhibitionofmir9decreasesosteosarcomacellproliferation
AT yonghuang inhibitionofmir9decreasesosteosarcomacellproliferation
AT jiajunqin inhibitionofmir9decreasesosteosarcomacellproliferation
AT yizhang inhibitionofmir9decreasesosteosarcomacellproliferation
AT haohu inhibitionofmir9decreasesosteosarcomacellproliferation