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PHTF2 regulates lipids metabolism in gastric cancer
Identification of hub genes and key pathways of gastric cancer was recognized to be essential to elucidate the tumorigenesis of GC. This study was aimed to identify the differentially expressed genes (DEGs) in GC via bioinformatics methods and their related pathways involved in the pathological proc...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7202541/ https://www.ncbi.nlm.nih.gov/pubmed/32335542 http://dx.doi.org/10.18632/aging.102995 |
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author | Chi, Yuhua Wang, Haiyan Wang, Fengsong Ding, Mingcui |
author_facet | Chi, Yuhua Wang, Haiyan Wang, Fengsong Ding, Mingcui |
author_sort | Chi, Yuhua |
collection | PubMed |
description | Identification of hub genes and key pathways of gastric cancer was recognized to be essential to elucidate the tumorigenesis of GC. This study was aimed to identify the differentially expressed genes (DEGs) in GC via bioinformatics methods and their related pathways involved in the pathological process of GC. Gene expression profile datasets acquired by microarray chips or RNA-seq were downloaded from GEO dataset and TCGA, and 298 differentially expressed genes was identified. The Gene Ontology (GO) and Kyoto Gene and Genomic Encyclopedia (KEGG) pathways of DEGs were then analyzed by the DAVID database to elucidate the potential molecular functions of DEGs. The protein-protein interaction (PPI) network of DEGs was further analyzed with the STRING database and PHTF2 was identified as a hub gene in the PPI network. Subsequently, PHTF2 was found to be highly expressed in different subtypes of gastric cancer tissues obtained from TCGA database or clinical patients, resulting with a poor prognosis. By GSEA, PHTF2 was found to significantly enrich the fatty acid metabolism pathway in gastric cancer. Moreover, PHTF2-regulated lipids metabolism significantly affected the tumorigenesis of GC cells. In summary, this work identified a new mechanism by which PHTF2 precipitated in the pathological process of GC by regulating cellular lipid metabolism. |
format | Online Article Text |
id | pubmed-7202541 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Impact Journals |
record_format | MEDLINE/PubMed |
spelling | pubmed-72025412020-05-11 PHTF2 regulates lipids metabolism in gastric cancer Chi, Yuhua Wang, Haiyan Wang, Fengsong Ding, Mingcui Aging (Albany NY) Research Paper Identification of hub genes and key pathways of gastric cancer was recognized to be essential to elucidate the tumorigenesis of GC. This study was aimed to identify the differentially expressed genes (DEGs) in GC via bioinformatics methods and their related pathways involved in the pathological process of GC. Gene expression profile datasets acquired by microarray chips or RNA-seq were downloaded from GEO dataset and TCGA, and 298 differentially expressed genes was identified. The Gene Ontology (GO) and Kyoto Gene and Genomic Encyclopedia (KEGG) pathways of DEGs were then analyzed by the DAVID database to elucidate the potential molecular functions of DEGs. The protein-protein interaction (PPI) network of DEGs was further analyzed with the STRING database and PHTF2 was identified as a hub gene in the PPI network. Subsequently, PHTF2 was found to be highly expressed in different subtypes of gastric cancer tissues obtained from TCGA database or clinical patients, resulting with a poor prognosis. By GSEA, PHTF2 was found to significantly enrich the fatty acid metabolism pathway in gastric cancer. Moreover, PHTF2-regulated lipids metabolism significantly affected the tumorigenesis of GC cells. In summary, this work identified a new mechanism by which PHTF2 precipitated in the pathological process of GC by regulating cellular lipid metabolism. Impact Journals 2020-04-25 /pmc/articles/PMC7202541/ /pubmed/32335542 http://dx.doi.org/10.18632/aging.102995 Text en Copyright © 2020 Chi et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Chi, Yuhua Wang, Haiyan Wang, Fengsong Ding, Mingcui PHTF2 regulates lipids metabolism in gastric cancer |
title | PHTF2 regulates lipids metabolism in gastric cancer |
title_full | PHTF2 regulates lipids metabolism in gastric cancer |
title_fullStr | PHTF2 regulates lipids metabolism in gastric cancer |
title_full_unstemmed | PHTF2 regulates lipids metabolism in gastric cancer |
title_short | PHTF2 regulates lipids metabolism in gastric cancer |
title_sort | phtf2 regulates lipids metabolism in gastric cancer |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7202541/ https://www.ncbi.nlm.nih.gov/pubmed/32335542 http://dx.doi.org/10.18632/aging.102995 |
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