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PHTF2 regulates lipids metabolism in gastric cancer

Identification of hub genes and key pathways of gastric cancer was recognized to be essential to elucidate the tumorigenesis of GC. This study was aimed to identify the differentially expressed genes (DEGs) in GC via bioinformatics methods and their related pathways involved in the pathological proc...

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Autores principales: Chi, Yuhua, Wang, Haiyan, Wang, Fengsong, Ding, Mingcui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7202541/
https://www.ncbi.nlm.nih.gov/pubmed/32335542
http://dx.doi.org/10.18632/aging.102995
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author Chi, Yuhua
Wang, Haiyan
Wang, Fengsong
Ding, Mingcui
author_facet Chi, Yuhua
Wang, Haiyan
Wang, Fengsong
Ding, Mingcui
author_sort Chi, Yuhua
collection PubMed
description Identification of hub genes and key pathways of gastric cancer was recognized to be essential to elucidate the tumorigenesis of GC. This study was aimed to identify the differentially expressed genes (DEGs) in GC via bioinformatics methods and their related pathways involved in the pathological process of GC. Gene expression profile datasets acquired by microarray chips or RNA-seq were downloaded from GEO dataset and TCGA, and 298 differentially expressed genes was identified. The Gene Ontology (GO) and Kyoto Gene and Genomic Encyclopedia (KEGG) pathways of DEGs were then analyzed by the DAVID database to elucidate the potential molecular functions of DEGs. The protein-protein interaction (PPI) network of DEGs was further analyzed with the STRING database and PHTF2 was identified as a hub gene in the PPI network. Subsequently, PHTF2 was found to be highly expressed in different subtypes of gastric cancer tissues obtained from TCGA database or clinical patients, resulting with a poor prognosis. By GSEA, PHTF2 was found to significantly enrich the fatty acid metabolism pathway in gastric cancer. Moreover, PHTF2-regulated lipids metabolism significantly affected the tumorigenesis of GC cells. In summary, this work identified a new mechanism by which PHTF2 precipitated in the pathological process of GC by regulating cellular lipid metabolism.
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spelling pubmed-72025412020-05-11 PHTF2 regulates lipids metabolism in gastric cancer Chi, Yuhua Wang, Haiyan Wang, Fengsong Ding, Mingcui Aging (Albany NY) Research Paper Identification of hub genes and key pathways of gastric cancer was recognized to be essential to elucidate the tumorigenesis of GC. This study was aimed to identify the differentially expressed genes (DEGs) in GC via bioinformatics methods and their related pathways involved in the pathological process of GC. Gene expression profile datasets acquired by microarray chips or RNA-seq were downloaded from GEO dataset and TCGA, and 298 differentially expressed genes was identified. The Gene Ontology (GO) and Kyoto Gene and Genomic Encyclopedia (KEGG) pathways of DEGs were then analyzed by the DAVID database to elucidate the potential molecular functions of DEGs. The protein-protein interaction (PPI) network of DEGs was further analyzed with the STRING database and PHTF2 was identified as a hub gene in the PPI network. Subsequently, PHTF2 was found to be highly expressed in different subtypes of gastric cancer tissues obtained from TCGA database or clinical patients, resulting with a poor prognosis. By GSEA, PHTF2 was found to significantly enrich the fatty acid metabolism pathway in gastric cancer. Moreover, PHTF2-regulated lipids metabolism significantly affected the tumorigenesis of GC cells. In summary, this work identified a new mechanism by which PHTF2 precipitated in the pathological process of GC by regulating cellular lipid metabolism. Impact Journals 2020-04-25 /pmc/articles/PMC7202541/ /pubmed/32335542 http://dx.doi.org/10.18632/aging.102995 Text en Copyright © 2020 Chi et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Chi, Yuhua
Wang, Haiyan
Wang, Fengsong
Ding, Mingcui
PHTF2 regulates lipids metabolism in gastric cancer
title PHTF2 regulates lipids metabolism in gastric cancer
title_full PHTF2 regulates lipids metabolism in gastric cancer
title_fullStr PHTF2 regulates lipids metabolism in gastric cancer
title_full_unstemmed PHTF2 regulates lipids metabolism in gastric cancer
title_short PHTF2 regulates lipids metabolism in gastric cancer
title_sort phtf2 regulates lipids metabolism in gastric cancer
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7202541/
https://www.ncbi.nlm.nih.gov/pubmed/32335542
http://dx.doi.org/10.18632/aging.102995
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