Strand breaks and chemical modification of intracellular DNA induced by cold atmospheric pressure plasma irradiation

DNA damage in the A549 human lung cancer cell line treated with cold plasma irradiation was investigated. We confirmed that cold atmospheric plasma generated reactive oxygen and nitrogen species (RONS) in a liquid, and the intracellular RONS level was increased in plasma-irradiated cells. However, a...

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Autores principales: Kurita, Hirofumi, Haruta, Natsuki, Uchihashi, Yoshito, Seto, Takahito, Takashima, Kazunori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7202611/
https://www.ncbi.nlm.nih.gov/pubmed/32374749
http://dx.doi.org/10.1371/journal.pone.0232724
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author Kurita, Hirofumi
Haruta, Natsuki
Uchihashi, Yoshito
Seto, Takahito
Takashima, Kazunori
author_facet Kurita, Hirofumi
Haruta, Natsuki
Uchihashi, Yoshito
Seto, Takahito
Takashima, Kazunori
author_sort Kurita, Hirofumi
collection PubMed
description DNA damage in the A549 human lung cancer cell line treated with cold plasma irradiation was investigated. We confirmed that cold atmospheric plasma generated reactive oxygen and nitrogen species (RONS) in a liquid, and the intracellular RONS level was increased in plasma-irradiated cells. However, a notable decrease in cell viability was not observed 24 hours after plasma irradiation. Because RONS induce oxidative damage in cells, strand breaks and chemical modification of DNA in the cancer cells were investigated. We found that 8-oxoguanine (8-oxoG) formation as well as DNA strand breaks, which have been thoroughly investigated, were induced by plasma irradiation. In addition, up-regulation of 8-oxoG repair enzyme was observed after plasma irradiation.
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spelling pubmed-72026112020-05-12 Strand breaks and chemical modification of intracellular DNA induced by cold atmospheric pressure plasma irradiation Kurita, Hirofumi Haruta, Natsuki Uchihashi, Yoshito Seto, Takahito Takashima, Kazunori PLoS One Research Article DNA damage in the A549 human lung cancer cell line treated with cold plasma irradiation was investigated. We confirmed that cold atmospheric plasma generated reactive oxygen and nitrogen species (RONS) in a liquid, and the intracellular RONS level was increased in plasma-irradiated cells. However, a notable decrease in cell viability was not observed 24 hours after plasma irradiation. Because RONS induce oxidative damage in cells, strand breaks and chemical modification of DNA in the cancer cells were investigated. We found that 8-oxoguanine (8-oxoG) formation as well as DNA strand breaks, which have been thoroughly investigated, were induced by plasma irradiation. In addition, up-regulation of 8-oxoG repair enzyme was observed after plasma irradiation. Public Library of Science 2020-05-06 /pmc/articles/PMC7202611/ /pubmed/32374749 http://dx.doi.org/10.1371/journal.pone.0232724 Text en © 2020 Kurita et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Kurita, Hirofumi
Haruta, Natsuki
Uchihashi, Yoshito
Seto, Takahito
Takashima, Kazunori
Strand breaks and chemical modification of intracellular DNA induced by cold atmospheric pressure plasma irradiation
title Strand breaks and chemical modification of intracellular DNA induced by cold atmospheric pressure plasma irradiation
title_full Strand breaks and chemical modification of intracellular DNA induced by cold atmospheric pressure plasma irradiation
title_fullStr Strand breaks and chemical modification of intracellular DNA induced by cold atmospheric pressure plasma irradiation
title_full_unstemmed Strand breaks and chemical modification of intracellular DNA induced by cold atmospheric pressure plasma irradiation
title_short Strand breaks and chemical modification of intracellular DNA induced by cold atmospheric pressure plasma irradiation
title_sort strand breaks and chemical modification of intracellular dna induced by cold atmospheric pressure plasma irradiation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7202611/
https://www.ncbi.nlm.nih.gov/pubmed/32374749
http://dx.doi.org/10.1371/journal.pone.0232724
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