Magnetic particles for integrated nucleic acid purification, amplification and detection without pipetting

Nucleic acid amplification based detection plays an important role in food safety, environmental monitoring and clinical diagnosis. However, traditional nucleic acid detection process involves transferring liquid from one tube to another by pipetting. It requires trained persons, equipped labs and c...

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Detalles Bibliográficos
Autores principales: Chen, Yanju, Liu, Yang, Shi, Ya, Ping, Jianfeng, Wu, Jian, Chen, Huan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7202819/
https://www.ncbi.nlm.nih.gov/pubmed/32382202
http://dx.doi.org/10.1016/j.trac.2020.115912
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author Chen, Yanju
Liu, Yang
Shi, Ya
Ping, Jianfeng
Wu, Jian
Chen, Huan
author_facet Chen, Yanju
Liu, Yang
Shi, Ya
Ping, Jianfeng
Wu, Jian
Chen, Huan
author_sort Chen, Yanju
collection PubMed
description Nucleic acid amplification based detection plays an important role in food safety, environmental monitoring and clinical diagnosis. However, traditional nucleic acid detection process involves transferring liquid from one tube to another by pipetting. It requires trained persons, equipped labs and consumes lots of time. The ideal nucleic acid detection is integrated, closed, simplified and automated. Magnetic particles actuated by magnetic fields can efficiently adsorb nucleic acids and promote integrated nucleic acid assays without pipetting driven by pumps and centrifuges. We will comprehensively review magnetic particles assisted integrated system for nucleic acid detection and hope it can inspire further related study.
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spelling pubmed-72028192020-05-07 Magnetic particles for integrated nucleic acid purification, amplification and detection without pipetting Chen, Yanju Liu, Yang Shi, Ya Ping, Jianfeng Wu, Jian Chen, Huan Trends Analyt Chem Article Nucleic acid amplification based detection plays an important role in food safety, environmental monitoring and clinical diagnosis. However, traditional nucleic acid detection process involves transferring liquid from one tube to another by pipetting. It requires trained persons, equipped labs and consumes lots of time. The ideal nucleic acid detection is integrated, closed, simplified and automated. Magnetic particles actuated by magnetic fields can efficiently adsorb nucleic acids and promote integrated nucleic acid assays without pipetting driven by pumps and centrifuges. We will comprehensively review magnetic particles assisted integrated system for nucleic acid detection and hope it can inspire further related study. Elsevier B.V. 2020-06 2020-05-06 /pmc/articles/PMC7202819/ /pubmed/32382202 http://dx.doi.org/10.1016/j.trac.2020.115912 Text en © 2020 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Chen, Yanju
Liu, Yang
Shi, Ya
Ping, Jianfeng
Wu, Jian
Chen, Huan
Magnetic particles for integrated nucleic acid purification, amplification and detection without pipetting
title Magnetic particles for integrated nucleic acid purification, amplification and detection without pipetting
title_full Magnetic particles for integrated nucleic acid purification, amplification and detection without pipetting
title_fullStr Magnetic particles for integrated nucleic acid purification, amplification and detection without pipetting
title_full_unstemmed Magnetic particles for integrated nucleic acid purification, amplification and detection without pipetting
title_short Magnetic particles for integrated nucleic acid purification, amplification and detection without pipetting
title_sort magnetic particles for integrated nucleic acid purification, amplification and detection without pipetting
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7202819/
https://www.ncbi.nlm.nih.gov/pubmed/32382202
http://dx.doi.org/10.1016/j.trac.2020.115912
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