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The plant cuticle regulates apoplastic transport of salicylic acid during systemic acquired resistance

The plant cuticle is often considered a passive barrier from the environment. We show that the cuticle regulates active transport of the defense hormone salicylic acid (SA). SA, an important regulator of systemic acquired resistance (SAR), is preferentially transported from pathogen-infected to unin...

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Autores principales: Lim, Gah-Hyun, Liu, Huazhen, Yu, Keshun, Liu, Ruiying, Shine, M. B., Fernandez, Jessica, Burch-Smith, Tessa, Mobley, Justin K., McLetchie, Nicholas, Kachroo, Aardra, Kachroo, Pradeep
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7202870/
https://www.ncbi.nlm.nih.gov/pubmed/32494705
http://dx.doi.org/10.1126/sciadv.aaz0478
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author Lim, Gah-Hyun
Liu, Huazhen
Yu, Keshun
Liu, Ruiying
Shine, M. B.
Fernandez, Jessica
Burch-Smith, Tessa
Mobley, Justin K.
McLetchie, Nicholas
Kachroo, Aardra
Kachroo, Pradeep
author_facet Lim, Gah-Hyun
Liu, Huazhen
Yu, Keshun
Liu, Ruiying
Shine, M. B.
Fernandez, Jessica
Burch-Smith, Tessa
Mobley, Justin K.
McLetchie, Nicholas
Kachroo, Aardra
Kachroo, Pradeep
author_sort Lim, Gah-Hyun
collection PubMed
description The plant cuticle is often considered a passive barrier from the environment. We show that the cuticle regulates active transport of the defense hormone salicylic acid (SA). SA, an important regulator of systemic acquired resistance (SAR), is preferentially transported from pathogen-infected to uninfected parts via the apoplast. Apoplastic accumulation of SA, which precedes its accumulation in the cytosol, is driven by the pH gradient and deprotonation of SA. In cuticle-defective mutants, increased transpiration and reduced water potential preferentially routes SA to cuticle wax rather than to the apoplast. This results in defective long-distance transport of SA, which in turn impairs distal accumulation of the SAR-inducer pipecolic acid. High humidity reduces transpiration to restore systemic SA transport and, thereby, SAR in cuticle-defective mutants. Together, our results demonstrate that long-distance mobility of SA is essential for SAR and that partitioning of SA between the symplast and cuticle is regulated by transpiration.
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spelling pubmed-72028702020-06-02 The plant cuticle regulates apoplastic transport of salicylic acid during systemic acquired resistance Lim, Gah-Hyun Liu, Huazhen Yu, Keshun Liu, Ruiying Shine, M. B. Fernandez, Jessica Burch-Smith, Tessa Mobley, Justin K. McLetchie, Nicholas Kachroo, Aardra Kachroo, Pradeep Sci Adv Research Articles The plant cuticle is often considered a passive barrier from the environment. We show that the cuticle regulates active transport of the defense hormone salicylic acid (SA). SA, an important regulator of systemic acquired resistance (SAR), is preferentially transported from pathogen-infected to uninfected parts via the apoplast. Apoplastic accumulation of SA, which precedes its accumulation in the cytosol, is driven by the pH gradient and deprotonation of SA. In cuticle-defective mutants, increased transpiration and reduced water potential preferentially routes SA to cuticle wax rather than to the apoplast. This results in defective long-distance transport of SA, which in turn impairs distal accumulation of the SAR-inducer pipecolic acid. High humidity reduces transpiration to restore systemic SA transport and, thereby, SAR in cuticle-defective mutants. Together, our results demonstrate that long-distance mobility of SA is essential for SAR and that partitioning of SA between the symplast and cuticle is regulated by transpiration. American Association for the Advancement of Science 2020-05-06 /pmc/articles/PMC7202870/ /pubmed/32494705 http://dx.doi.org/10.1126/sciadv.aaz0478 Text en Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (http://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
spellingShingle Research Articles
Lim, Gah-Hyun
Liu, Huazhen
Yu, Keshun
Liu, Ruiying
Shine, M. B.
Fernandez, Jessica
Burch-Smith, Tessa
Mobley, Justin K.
McLetchie, Nicholas
Kachroo, Aardra
Kachroo, Pradeep
The plant cuticle regulates apoplastic transport of salicylic acid during systemic acquired resistance
title The plant cuticle regulates apoplastic transport of salicylic acid during systemic acquired resistance
title_full The plant cuticle regulates apoplastic transport of salicylic acid during systemic acquired resistance
title_fullStr The plant cuticle regulates apoplastic transport of salicylic acid during systemic acquired resistance
title_full_unstemmed The plant cuticle regulates apoplastic transport of salicylic acid during systemic acquired resistance
title_short The plant cuticle regulates apoplastic transport of salicylic acid during systemic acquired resistance
title_sort plant cuticle regulates apoplastic transport of salicylic acid during systemic acquired resistance
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7202870/
https://www.ncbi.nlm.nih.gov/pubmed/32494705
http://dx.doi.org/10.1126/sciadv.aaz0478
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