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Particle-based artificial three-dimensional stem cell spheroids for revascularization of ischemic diseases

Development of new approaches to biomimetically reconstruct vasculature networks remains challenging in regenerative medicine. We introduce a particle-based artificial stem cell spheroid (ASSP) technology that recapitulates paracrine functions of three-dimensional (3D) SSPs for vasculature regenerat...

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Autores principales: Zhang, Ran, Luo, Wenya, Zhang, Yue, Zhu, Dashuai, Midgley, Adam C., Song, Hao, Khalique, Anila, Zhang, Haoqi, Zhuang, Jie, Kong, Deling, Huang, Xinglu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7202876/
https://www.ncbi.nlm.nih.gov/pubmed/32494716
http://dx.doi.org/10.1126/sciadv.aaz8011
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author Zhang, Ran
Luo, Wenya
Zhang, Yue
Zhu, Dashuai
Midgley, Adam C.
Song, Hao
Khalique, Anila
Zhang, Haoqi
Zhuang, Jie
Kong, Deling
Huang, Xinglu
author_facet Zhang, Ran
Luo, Wenya
Zhang, Yue
Zhu, Dashuai
Midgley, Adam C.
Song, Hao
Khalique, Anila
Zhang, Haoqi
Zhuang, Jie
Kong, Deling
Huang, Xinglu
author_sort Zhang, Ran
collection PubMed
description Development of new approaches to biomimetically reconstruct vasculature networks remains challenging in regenerative medicine. We introduce a particle-based artificial stem cell spheroid (ASSP) technology that recapitulates paracrine functions of three-dimensional (3D) SSPs for vasculature regeneration. Specifically, we used a facile method to induce the aggregation of stem cells into 3D spheroids, which benefited from hypoxia microenvironment–driven and enhanced secretion of proangiogenic bioactive factors. Furthermore, we artificially reconstructed 3D spheroids (i.e., ASSP) by integration of SSP-secreted factors into micro-/nanoparticles with cell membrane–derived surface coatings. The easily controllable sizes of the ASSP particles provided superior revascularization effects on the ischemic tissues in hindlimb ischemia models through local administration of ASSP microparticles and in myocardial infarction models via the systemic delivery of ASSP nanoparticles. The strategy offers a promising therapeutic option for ischemic tissue regeneration and addresses issues faced by the bottlenecked development in the delivery of stem cell therapies.
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spelling pubmed-72028762020-06-02 Particle-based artificial three-dimensional stem cell spheroids for revascularization of ischemic diseases Zhang, Ran Luo, Wenya Zhang, Yue Zhu, Dashuai Midgley, Adam C. Song, Hao Khalique, Anila Zhang, Haoqi Zhuang, Jie Kong, Deling Huang, Xinglu Sci Adv Research Articles Development of new approaches to biomimetically reconstruct vasculature networks remains challenging in regenerative medicine. We introduce a particle-based artificial stem cell spheroid (ASSP) technology that recapitulates paracrine functions of three-dimensional (3D) SSPs for vasculature regeneration. Specifically, we used a facile method to induce the aggregation of stem cells into 3D spheroids, which benefited from hypoxia microenvironment–driven and enhanced secretion of proangiogenic bioactive factors. Furthermore, we artificially reconstructed 3D spheroids (i.e., ASSP) by integration of SSP-secreted factors into micro-/nanoparticles with cell membrane–derived surface coatings. The easily controllable sizes of the ASSP particles provided superior revascularization effects on the ischemic tissues in hindlimb ischemia models through local administration of ASSP microparticles and in myocardial infarction models via the systemic delivery of ASSP nanoparticles. The strategy offers a promising therapeutic option for ischemic tissue regeneration and addresses issues faced by the bottlenecked development in the delivery of stem cell therapies. American Association for the Advancement of Science 2020-05-06 /pmc/articles/PMC7202876/ /pubmed/32494716 http://dx.doi.org/10.1126/sciadv.aaz8011 Text en Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (http://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
spellingShingle Research Articles
Zhang, Ran
Luo, Wenya
Zhang, Yue
Zhu, Dashuai
Midgley, Adam C.
Song, Hao
Khalique, Anila
Zhang, Haoqi
Zhuang, Jie
Kong, Deling
Huang, Xinglu
Particle-based artificial three-dimensional stem cell spheroids for revascularization of ischemic diseases
title Particle-based artificial three-dimensional stem cell spheroids for revascularization of ischemic diseases
title_full Particle-based artificial three-dimensional stem cell spheroids for revascularization of ischemic diseases
title_fullStr Particle-based artificial three-dimensional stem cell spheroids for revascularization of ischemic diseases
title_full_unstemmed Particle-based artificial three-dimensional stem cell spheroids for revascularization of ischemic diseases
title_short Particle-based artificial three-dimensional stem cell spheroids for revascularization of ischemic diseases
title_sort particle-based artificial three-dimensional stem cell spheroids for revascularization of ischemic diseases
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7202876/
https://www.ncbi.nlm.nih.gov/pubmed/32494716
http://dx.doi.org/10.1126/sciadv.aaz8011
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