Cargando…

Identification and profiling of microRNAs and differentially expressed genes during anther development between a genetic male-sterile mutant and its wildtype cotton via high-throughput RNA sequencing

Genetic male sterility (GMS) facilitates hybrid seed production in crops including cotton (Gossypium hirsutum). However, the genetic and molecular mechanisms specifically involved in this developmental process are poorly understood. In this study, small RNA sequencing, degradome sequencing, and tran...

Descripción completa

Detalles Bibliográficos
Autores principales: Yu, Dingwei, Li, Libei, Wei, Hengling, Yu, Shuxun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7203095/
https://www.ncbi.nlm.nih.gov/pubmed/32172356
http://dx.doi.org/10.1007/s00438-020-01656-y
_version_ 1783529809802952704
author Yu, Dingwei
Li, Libei
Wei, Hengling
Yu, Shuxun
author_facet Yu, Dingwei
Li, Libei
Wei, Hengling
Yu, Shuxun
author_sort Yu, Dingwei
collection PubMed
description Genetic male sterility (GMS) facilitates hybrid seed production in crops including cotton (Gossypium hirsutum). However, the genetic and molecular mechanisms specifically involved in this developmental process are poorly understood. In this study, small RNA sequencing, degradome sequencing, and transcriptome sequencing were performed to analyze miRNAs and their target genes during anther development in a GMS mutant (‘Dong A’) and its fertile wildtype (WT). A total of 80 known and 220 novel miRNAs were identified, 71 of which showed differential expressions during anther development. A further degradome sequencing revealed a total of 117 candidate target genes cleaved by 16 known and 36 novel miRNAs. Based on RNA-seq, 24, 11, and 21 predicted target genes showed expression correlations with the corresponding miRNAs at the meiosis, tetrad and uninucleate stages, respectively. In addition, a large number of differentially expressed genes were identified, most of which were involved in sucrose and starch metabolism, carbohydrate metabolism, and plant hormone signal transduction based on Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. The results of our study provide valuable information for further functional investigations of the important miRNAs and target genes involved in genetic male sterility and advance our understanding of miRNA regulatory functions during cotton anther development. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00438-020-01656-y) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-7203095
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Springer Berlin Heidelberg
record_format MEDLINE/PubMed
spelling pubmed-72030952020-05-07 Identification and profiling of microRNAs and differentially expressed genes during anther development between a genetic male-sterile mutant and its wildtype cotton via high-throughput RNA sequencing Yu, Dingwei Li, Libei Wei, Hengling Yu, Shuxun Mol Genet Genomics Original Article Genetic male sterility (GMS) facilitates hybrid seed production in crops including cotton (Gossypium hirsutum). However, the genetic and molecular mechanisms specifically involved in this developmental process are poorly understood. In this study, small RNA sequencing, degradome sequencing, and transcriptome sequencing were performed to analyze miRNAs and their target genes during anther development in a GMS mutant (‘Dong A’) and its fertile wildtype (WT). A total of 80 known and 220 novel miRNAs were identified, 71 of which showed differential expressions during anther development. A further degradome sequencing revealed a total of 117 candidate target genes cleaved by 16 known and 36 novel miRNAs. Based on RNA-seq, 24, 11, and 21 predicted target genes showed expression correlations with the corresponding miRNAs at the meiosis, tetrad and uninucleate stages, respectively. In addition, a large number of differentially expressed genes were identified, most of which were involved in sucrose and starch metabolism, carbohydrate metabolism, and plant hormone signal transduction based on Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. The results of our study provide valuable information for further functional investigations of the important miRNAs and target genes involved in genetic male sterility and advance our understanding of miRNA regulatory functions during cotton anther development. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00438-020-01656-y) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2020-03-14 2020 /pmc/articles/PMC7203095/ /pubmed/32172356 http://dx.doi.org/10.1007/s00438-020-01656-y Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Original Article
Yu, Dingwei
Li, Libei
Wei, Hengling
Yu, Shuxun
Identification and profiling of microRNAs and differentially expressed genes during anther development between a genetic male-sterile mutant and its wildtype cotton via high-throughput RNA sequencing
title Identification and profiling of microRNAs and differentially expressed genes during anther development between a genetic male-sterile mutant and its wildtype cotton via high-throughput RNA sequencing
title_full Identification and profiling of microRNAs and differentially expressed genes during anther development between a genetic male-sterile mutant and its wildtype cotton via high-throughput RNA sequencing
title_fullStr Identification and profiling of microRNAs and differentially expressed genes during anther development between a genetic male-sterile mutant and its wildtype cotton via high-throughput RNA sequencing
title_full_unstemmed Identification and profiling of microRNAs and differentially expressed genes during anther development between a genetic male-sterile mutant and its wildtype cotton via high-throughput RNA sequencing
title_short Identification and profiling of microRNAs and differentially expressed genes during anther development between a genetic male-sterile mutant and its wildtype cotton via high-throughput RNA sequencing
title_sort identification and profiling of micrornas and differentially expressed genes during anther development between a genetic male-sterile mutant and its wildtype cotton via high-throughput rna sequencing
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7203095/
https://www.ncbi.nlm.nih.gov/pubmed/32172356
http://dx.doi.org/10.1007/s00438-020-01656-y
work_keys_str_mv AT yudingwei identificationandprofilingofmicrornasanddifferentiallyexpressedgenesduringantherdevelopmentbetweenageneticmalesterilemutantanditswildtypecottonviahighthroughputrnasequencing
AT lilibei identificationandprofilingofmicrornasanddifferentiallyexpressedgenesduringantherdevelopmentbetweenageneticmalesterilemutantanditswildtypecottonviahighthroughputrnasequencing
AT weihengling identificationandprofilingofmicrornasanddifferentiallyexpressedgenesduringantherdevelopmentbetweenageneticmalesterilemutantanditswildtypecottonviahighthroughputrnasequencing
AT yushuxun identificationandprofilingofmicrornasanddifferentiallyexpressedgenesduringantherdevelopmentbetweenageneticmalesterilemutantanditswildtypecottonviahighthroughputrnasequencing