Cargando…

Fluorescence spectroscopy and chemometrics for simultaneous monitoring of cell concentration, chlorophyll and fatty acids in Nannochloropsis oceanica

Online monitoring of algal biotechnological processes still requires development to support economic sustainability. In this work, fluorescence spectroscopy coupled with chemometric modelling is studied to monitor simultaneously several compounds of interest, such as chlorophyll and fatty acids, but...

Descripción completa

Detalles Bibliográficos
Autores principales: Sá, Marta, Bertinetto, Carlo G., Ferrer-Ledo, Narcís, Jansen, Jeroen J., Wijffels, Rene, Crespo, João G., Barbosa, Maria, Galinha, Claudia F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7203222/
https://www.ncbi.nlm.nih.gov/pubmed/32376848
http://dx.doi.org/10.1038/s41598-020-64628-7
_version_ 1783529838875770880
author Sá, Marta
Bertinetto, Carlo G.
Ferrer-Ledo, Narcís
Jansen, Jeroen J.
Wijffels, Rene
Crespo, João G.
Barbosa, Maria
Galinha, Claudia F.
author_facet Sá, Marta
Bertinetto, Carlo G.
Ferrer-Ledo, Narcís
Jansen, Jeroen J.
Wijffels, Rene
Crespo, João G.
Barbosa, Maria
Galinha, Claudia F.
author_sort Sá, Marta
collection PubMed
description Online monitoring of algal biotechnological processes still requires development to support economic sustainability. In this work, fluorescence spectroscopy coupled with chemometric modelling is studied to monitor simultaneously several compounds of interest, such as chlorophyll and fatty acids, but also the biomass as a whole (cell concentration). Fluorescence excitation-emission matrices (EEM) were acquired in experiments where different environmental growing parameters were tested, namely light regime, temperature and nitrogen (replete or deplete medium). The prediction models developed have a high R(2) for the validation data set for all five parameters monitored, specifically cell concentration (0.66), chlorophyll (0.78), and fatty acid as total (0.78), saturated (0.81) and unsaturated (0.74). Regression coefficient maps of the models show the importance of the pigment region for all outputs studied, and the protein-like fluorescence region for the cell concentration. These results demonstrate for the first time the potential of fluorescence spectroscopy for in vivo and real-time monitoring of these key performance parameters during Nannochloropsis oceanica cultivation.
format Online
Article
Text
id pubmed-7203222
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-72032222020-05-15 Fluorescence spectroscopy and chemometrics for simultaneous monitoring of cell concentration, chlorophyll and fatty acids in Nannochloropsis oceanica Sá, Marta Bertinetto, Carlo G. Ferrer-Ledo, Narcís Jansen, Jeroen J. Wijffels, Rene Crespo, João G. Barbosa, Maria Galinha, Claudia F. Sci Rep Article Online monitoring of algal biotechnological processes still requires development to support economic sustainability. In this work, fluorescence spectroscopy coupled with chemometric modelling is studied to monitor simultaneously several compounds of interest, such as chlorophyll and fatty acids, but also the biomass as a whole (cell concentration). Fluorescence excitation-emission matrices (EEM) were acquired in experiments where different environmental growing parameters were tested, namely light regime, temperature and nitrogen (replete or deplete medium). The prediction models developed have a high R(2) for the validation data set for all five parameters monitored, specifically cell concentration (0.66), chlorophyll (0.78), and fatty acid as total (0.78), saturated (0.81) and unsaturated (0.74). Regression coefficient maps of the models show the importance of the pigment region for all outputs studied, and the protein-like fluorescence region for the cell concentration. These results demonstrate for the first time the potential of fluorescence spectroscopy for in vivo and real-time monitoring of these key performance parameters during Nannochloropsis oceanica cultivation. Nature Publishing Group UK 2020-05-06 /pmc/articles/PMC7203222/ /pubmed/32376848 http://dx.doi.org/10.1038/s41598-020-64628-7 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Sá, Marta
Bertinetto, Carlo G.
Ferrer-Ledo, Narcís
Jansen, Jeroen J.
Wijffels, Rene
Crespo, João G.
Barbosa, Maria
Galinha, Claudia F.
Fluorescence spectroscopy and chemometrics for simultaneous monitoring of cell concentration, chlorophyll and fatty acids in Nannochloropsis oceanica
title Fluorescence spectroscopy and chemometrics for simultaneous monitoring of cell concentration, chlorophyll and fatty acids in Nannochloropsis oceanica
title_full Fluorescence spectroscopy and chemometrics for simultaneous monitoring of cell concentration, chlorophyll and fatty acids in Nannochloropsis oceanica
title_fullStr Fluorescence spectroscopy and chemometrics for simultaneous monitoring of cell concentration, chlorophyll and fatty acids in Nannochloropsis oceanica
title_full_unstemmed Fluorescence spectroscopy and chemometrics for simultaneous monitoring of cell concentration, chlorophyll and fatty acids in Nannochloropsis oceanica
title_short Fluorescence spectroscopy and chemometrics for simultaneous monitoring of cell concentration, chlorophyll and fatty acids in Nannochloropsis oceanica
title_sort fluorescence spectroscopy and chemometrics for simultaneous monitoring of cell concentration, chlorophyll and fatty acids in nannochloropsis oceanica
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7203222/
https://www.ncbi.nlm.nih.gov/pubmed/32376848
http://dx.doi.org/10.1038/s41598-020-64628-7
work_keys_str_mv AT samarta fluorescencespectroscopyandchemometricsforsimultaneousmonitoringofcellconcentrationchlorophyllandfattyacidsinnannochloropsisoceanica
AT bertinettocarlog fluorescencespectroscopyandchemometricsforsimultaneousmonitoringofcellconcentrationchlorophyllandfattyacidsinnannochloropsisoceanica
AT ferrerledonarcis fluorescencespectroscopyandchemometricsforsimultaneousmonitoringofcellconcentrationchlorophyllandfattyacidsinnannochloropsisoceanica
AT jansenjeroenj fluorescencespectroscopyandchemometricsforsimultaneousmonitoringofcellconcentrationchlorophyllandfattyacidsinnannochloropsisoceanica
AT wijffelsrene fluorescencespectroscopyandchemometricsforsimultaneousmonitoringofcellconcentrationchlorophyllandfattyacidsinnannochloropsisoceanica
AT crespojoaog fluorescencespectroscopyandchemometricsforsimultaneousmonitoringofcellconcentrationchlorophyllandfattyacidsinnannochloropsisoceanica
AT barbosamaria fluorescencespectroscopyandchemometricsforsimultaneousmonitoringofcellconcentrationchlorophyllandfattyacidsinnannochloropsisoceanica
AT galinhaclaudiaf fluorescencespectroscopyandchemometricsforsimultaneousmonitoringofcellconcentrationchlorophyllandfattyacidsinnannochloropsisoceanica