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Interactions Between Thiamethoxam and Deformed Wing Virus Can Drastically Impair Flight Behavior of Honey Bees

Exposure to multiple stress factors is believed to contribute to honey bee colony decline. However, little is known about how co-exposure to stress factors can alter the survival and behavior of free-living honey bees in colony conditions. We therefore studied the potential interaction between a neo...

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Autores principales: Coulon, Marianne, Dalmon, Anne, Di Prisco, Gennaro, Prado, Alberto, Arban, Florine, Dubois, Eric, Ribière-Chabert, Magali, Alaux, Cedric, Thiéry, Richard, Le Conte, Yves
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7203464/
https://www.ncbi.nlm.nih.gov/pubmed/32425910
http://dx.doi.org/10.3389/fmicb.2020.00766
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author Coulon, Marianne
Dalmon, Anne
Di Prisco, Gennaro
Prado, Alberto
Arban, Florine
Dubois, Eric
Ribière-Chabert, Magali
Alaux, Cedric
Thiéry, Richard
Le Conte, Yves
author_facet Coulon, Marianne
Dalmon, Anne
Di Prisco, Gennaro
Prado, Alberto
Arban, Florine
Dubois, Eric
Ribière-Chabert, Magali
Alaux, Cedric
Thiéry, Richard
Le Conte, Yves
author_sort Coulon, Marianne
collection PubMed
description Exposure to multiple stress factors is believed to contribute to honey bee colony decline. However, little is known about how co-exposure to stress factors can alter the survival and behavior of free-living honey bees in colony conditions. We therefore studied the potential interaction between a neonicotinoid pesticide, thiamethoxam, and a highly prevalent honey bee pathogen, Deformed wing virus (DWV). For this purpose, tagged bees were exposed to DWV by feeding or injection, and/or to field-relevant doses of thiamethoxam, then left in colonies equipped with optical bee counters to monitor flight activity. DWV loads and the expression of immune genes were quantified. A reduction in vitellogenin expression level was observed in DWV-injected bees and was associated with precocious onset of foraging. Combined exposure to DWV and thiamethoxam did not result in higher DWV loads compared to bees only exposed to DWV, but induced precocious foraging, increased the risk of not returning to the hive after the first flight, and decreased survival when compared to single stress exposures. We therefore provided the first evidence for deleterious interactions between DWV and thiamethoxam in natural conditions.
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spelling pubmed-72034642020-05-18 Interactions Between Thiamethoxam and Deformed Wing Virus Can Drastically Impair Flight Behavior of Honey Bees Coulon, Marianne Dalmon, Anne Di Prisco, Gennaro Prado, Alberto Arban, Florine Dubois, Eric Ribière-Chabert, Magali Alaux, Cedric Thiéry, Richard Le Conte, Yves Front Microbiol Microbiology Exposure to multiple stress factors is believed to contribute to honey bee colony decline. However, little is known about how co-exposure to stress factors can alter the survival and behavior of free-living honey bees in colony conditions. We therefore studied the potential interaction between a neonicotinoid pesticide, thiamethoxam, and a highly prevalent honey bee pathogen, Deformed wing virus (DWV). For this purpose, tagged bees were exposed to DWV by feeding or injection, and/or to field-relevant doses of thiamethoxam, then left in colonies equipped with optical bee counters to monitor flight activity. DWV loads and the expression of immune genes were quantified. A reduction in vitellogenin expression level was observed in DWV-injected bees and was associated with precocious onset of foraging. Combined exposure to DWV and thiamethoxam did not result in higher DWV loads compared to bees only exposed to DWV, but induced precocious foraging, increased the risk of not returning to the hive after the first flight, and decreased survival when compared to single stress exposures. We therefore provided the first evidence for deleterious interactions between DWV and thiamethoxam in natural conditions. Frontiers Media S.A. 2020-04-30 /pmc/articles/PMC7203464/ /pubmed/32425910 http://dx.doi.org/10.3389/fmicb.2020.00766 Text en Copyright © 2020 Coulon, Dalmon, Di Prisco, Prado, Arban, Dubois, Ribière-Chabert, Alaux, Thiéry and Le Conte. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Coulon, Marianne
Dalmon, Anne
Di Prisco, Gennaro
Prado, Alberto
Arban, Florine
Dubois, Eric
Ribière-Chabert, Magali
Alaux, Cedric
Thiéry, Richard
Le Conte, Yves
Interactions Between Thiamethoxam and Deformed Wing Virus Can Drastically Impair Flight Behavior of Honey Bees
title Interactions Between Thiamethoxam and Deformed Wing Virus Can Drastically Impair Flight Behavior of Honey Bees
title_full Interactions Between Thiamethoxam and Deformed Wing Virus Can Drastically Impair Flight Behavior of Honey Bees
title_fullStr Interactions Between Thiamethoxam and Deformed Wing Virus Can Drastically Impair Flight Behavior of Honey Bees
title_full_unstemmed Interactions Between Thiamethoxam and Deformed Wing Virus Can Drastically Impair Flight Behavior of Honey Bees
title_short Interactions Between Thiamethoxam and Deformed Wing Virus Can Drastically Impair Flight Behavior of Honey Bees
title_sort interactions between thiamethoxam and deformed wing virus can drastically impair flight behavior of honey bees
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7203464/
https://www.ncbi.nlm.nih.gov/pubmed/32425910
http://dx.doi.org/10.3389/fmicb.2020.00766
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