Cargando…

Peptide inhibitors of tembusu virus infection derived from the envelope protein

The outbreak and spread of Tembusu virus (TMUV) has caused very large losses in the waterfowl-breeding industry since 2010. The viral envelope (E) protein, the principal surface protein of viral particles, plays a vital role in viral entry and fusion. In this study, two peptides derived from domain...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhao, Dongmin, Zhang, Lijiao, Han, Kaikai, Liu, Qingtao, Yang, Jing, Huang, Xinmei, Liu, Yuzhuo, Li, Yin, Zhao, Peng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7204726/
https://www.ncbi.nlm.nih.gov/pubmed/32456819
http://dx.doi.org/10.1016/j.vetmic.2020.108708
_version_ 1783530106345488384
author Zhao, Dongmin
Zhang, Lijiao
Han, Kaikai
Liu, Qingtao
Yang, Jing
Huang, Xinmei
Liu, Yuzhuo
Li, Yin
Zhao, Peng
author_facet Zhao, Dongmin
Zhang, Lijiao
Han, Kaikai
Liu, Qingtao
Yang, Jing
Huang, Xinmei
Liu, Yuzhuo
Li, Yin
Zhao, Peng
author_sort Zhao, Dongmin
collection PubMed
description The outbreak and spread of Tembusu virus (TMUV) has caused very large losses in the waterfowl-breeding industry since 2010. The viral envelope (E) protein, the principal surface protein of viral particles, plays a vital role in viral entry and fusion. In this study, two peptides derived from domain II (DII) and the stem of the TMUV envelope protein, TP1 and TP2, respectively, were tested for their antiviral activity. TP1 and TP2 inhibited TMUV infection in BHK-21 cells, and their 50% inhibitory concentrations (IC(50)) were 14.19 mg/L and 7.64 mg/L, respectively. Viral inhibition assays in different cell lines of avian origin showed that the inhibitory effects of TP1 and TP2 are not cell type dependent. Moreover, TP2 also exhibited inhibitory activity against Japanese encephalitis virus (JEV) infection. The two peptides inhibited antibody-mediated TMUV infection of duck peripheral blood lymphocytes. Co-immunoprecipitation assays and indirect enzyme-linked immunosorbent assays (ELISAs) indicated that both peptides interact with the surface of the TMUV virion. RNase digestion assays confirmed the release of viral RNA following incubation with TP1, while incubation with TP1 or TP2 interfered with the binding between TMUV and cells. Taken together, these results show that TP1 and TP2 may be developed into antiviral treatments against TMUV infection.
format Online
Article
Text
id pubmed-7204726
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Published by Elsevier B.V.
record_format MEDLINE/PubMed
spelling pubmed-72047262020-05-07 Peptide inhibitors of tembusu virus infection derived from the envelope protein Zhao, Dongmin Zhang, Lijiao Han, Kaikai Liu, Qingtao Yang, Jing Huang, Xinmei Liu, Yuzhuo Li, Yin Zhao, Peng Vet Microbiol Article The outbreak and spread of Tembusu virus (TMUV) has caused very large losses in the waterfowl-breeding industry since 2010. The viral envelope (E) protein, the principal surface protein of viral particles, plays a vital role in viral entry and fusion. In this study, two peptides derived from domain II (DII) and the stem of the TMUV envelope protein, TP1 and TP2, respectively, were tested for their antiviral activity. TP1 and TP2 inhibited TMUV infection in BHK-21 cells, and their 50% inhibitory concentrations (IC(50)) were 14.19 mg/L and 7.64 mg/L, respectively. Viral inhibition assays in different cell lines of avian origin showed that the inhibitory effects of TP1 and TP2 are not cell type dependent. Moreover, TP2 also exhibited inhibitory activity against Japanese encephalitis virus (JEV) infection. The two peptides inhibited antibody-mediated TMUV infection of duck peripheral blood lymphocytes. Co-immunoprecipitation assays and indirect enzyme-linked immunosorbent assays (ELISAs) indicated that both peptides interact with the surface of the TMUV virion. RNase digestion assays confirmed the release of viral RNA following incubation with TP1, while incubation with TP1 or TP2 interfered with the binding between TMUV and cells. Taken together, these results show that TP1 and TP2 may be developed into antiviral treatments against TMUV infection. Published by Elsevier B.V. 2020-06 2020-05-07 /pmc/articles/PMC7204726/ /pubmed/32456819 http://dx.doi.org/10.1016/j.vetmic.2020.108708 Text en © 2020 Published by Elsevier B.V. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Zhao, Dongmin
Zhang, Lijiao
Han, Kaikai
Liu, Qingtao
Yang, Jing
Huang, Xinmei
Liu, Yuzhuo
Li, Yin
Zhao, Peng
Peptide inhibitors of tembusu virus infection derived from the envelope protein
title Peptide inhibitors of tembusu virus infection derived from the envelope protein
title_full Peptide inhibitors of tembusu virus infection derived from the envelope protein
title_fullStr Peptide inhibitors of tembusu virus infection derived from the envelope protein
title_full_unstemmed Peptide inhibitors of tembusu virus infection derived from the envelope protein
title_short Peptide inhibitors of tembusu virus infection derived from the envelope protein
title_sort peptide inhibitors of tembusu virus infection derived from the envelope protein
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7204726/
https://www.ncbi.nlm.nih.gov/pubmed/32456819
http://dx.doi.org/10.1016/j.vetmic.2020.108708
work_keys_str_mv AT zhaodongmin peptideinhibitorsoftembusuvirusinfectionderivedfromtheenvelopeprotein
AT zhanglijiao peptideinhibitorsoftembusuvirusinfectionderivedfromtheenvelopeprotein
AT hankaikai peptideinhibitorsoftembusuvirusinfectionderivedfromtheenvelopeprotein
AT liuqingtao peptideinhibitorsoftembusuvirusinfectionderivedfromtheenvelopeprotein
AT yangjing peptideinhibitorsoftembusuvirusinfectionderivedfromtheenvelopeprotein
AT huangxinmei peptideinhibitorsoftembusuvirusinfectionderivedfromtheenvelopeprotein
AT liuyuzhuo peptideinhibitorsoftembusuvirusinfectionderivedfromtheenvelopeprotein
AT liyin peptideinhibitorsoftembusuvirusinfectionderivedfromtheenvelopeprotein
AT zhaopeng peptideinhibitorsoftembusuvirusinfectionderivedfromtheenvelopeprotein