Cargando…

Leveraging plastomes for comparative analysis and phylogenomic inference within Scutellarioideae (Lamiaceae)

Scutellaria, or skullcaps, are medicinally important herbs in China, India, Japan, and elsewhere. Though Scutellaria is the second largest and one of the more taxonomically challenging genera within Lamiaceae, few molecular systematic studies have been undertaken within the genus; in part due to a p...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhao, Fei, Li, Bo, Drew, Bryan T., Chen, Ya-Ping, Wang, Qiang, Yu, Wen-Bin, Liu, En-De, Salmaki, Yasaman, Peng, Hua, Xiang, Chun-Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7205251/
https://www.ncbi.nlm.nih.gov/pubmed/32379799
http://dx.doi.org/10.1371/journal.pone.0232602
_version_ 1783530208835403776
author Zhao, Fei
Li, Bo
Drew, Bryan T.
Chen, Ya-Ping
Wang, Qiang
Yu, Wen-Bin
Liu, En-De
Salmaki, Yasaman
Peng, Hua
Xiang, Chun-Lei
author_facet Zhao, Fei
Li, Bo
Drew, Bryan T.
Chen, Ya-Ping
Wang, Qiang
Yu, Wen-Bin
Liu, En-De
Salmaki, Yasaman
Peng, Hua
Xiang, Chun-Lei
author_sort Zhao, Fei
collection PubMed
description Scutellaria, or skullcaps, are medicinally important herbs in China, India, Japan, and elsewhere. Though Scutellaria is the second largest and one of the more taxonomically challenging genera within Lamiaceae, few molecular systematic studies have been undertaken within the genus; in part due to a paucity of available informative markers. The lack of informative molecular markers for Scutellaria hinders our ability to accurately and robustly reconstruct phylogenetic relationships, which hampers our understanding of the diversity, phylogeny, and evolutionary history of this cosmopolitan genus. Comparative analyses of 15 plastomes, representing 14 species of subfamily Scutellarioideae, indicate that plastomes within Scutellarioideae contain about 151,000 nucleotides, and possess a typical quadripartite structure. In total, 590 simple sequence repeats, 489 longer repeats, and 16 hyper-variable regions were identified from the 15 plastomes. Phylogenetic relationships among the 14 species representing four of the five genera of Scutellarioideae were resolved with high support values, but the current infrageneric classification of Scutellaria was not supported in all analyses. Complete plastome sequences provide better resolution at an interspecific level than using few to several plastid markers in phylogenetic reconstruction. The data presented here will serve as a foundation to facilitate DNA barcoding, species identification, and systematic research within Scutellaria, which is an important medicinal plant resource worldwide.
format Online
Article
Text
id pubmed-7205251
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-72052512020-05-12 Leveraging plastomes for comparative analysis and phylogenomic inference within Scutellarioideae (Lamiaceae) Zhao, Fei Li, Bo Drew, Bryan T. Chen, Ya-Ping Wang, Qiang Yu, Wen-Bin Liu, En-De Salmaki, Yasaman Peng, Hua Xiang, Chun-Lei PLoS One Research Article Scutellaria, or skullcaps, are medicinally important herbs in China, India, Japan, and elsewhere. Though Scutellaria is the second largest and one of the more taxonomically challenging genera within Lamiaceae, few molecular systematic studies have been undertaken within the genus; in part due to a paucity of available informative markers. The lack of informative molecular markers for Scutellaria hinders our ability to accurately and robustly reconstruct phylogenetic relationships, which hampers our understanding of the diversity, phylogeny, and evolutionary history of this cosmopolitan genus. Comparative analyses of 15 plastomes, representing 14 species of subfamily Scutellarioideae, indicate that plastomes within Scutellarioideae contain about 151,000 nucleotides, and possess a typical quadripartite structure. In total, 590 simple sequence repeats, 489 longer repeats, and 16 hyper-variable regions were identified from the 15 plastomes. Phylogenetic relationships among the 14 species representing four of the five genera of Scutellarioideae were resolved with high support values, but the current infrageneric classification of Scutellaria was not supported in all analyses. Complete plastome sequences provide better resolution at an interspecific level than using few to several plastid markers in phylogenetic reconstruction. The data presented here will serve as a foundation to facilitate DNA barcoding, species identification, and systematic research within Scutellaria, which is an important medicinal plant resource worldwide. Public Library of Science 2020-05-07 /pmc/articles/PMC7205251/ /pubmed/32379799 http://dx.doi.org/10.1371/journal.pone.0232602 Text en © 2020 Zhao et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Zhao, Fei
Li, Bo
Drew, Bryan T.
Chen, Ya-Ping
Wang, Qiang
Yu, Wen-Bin
Liu, En-De
Salmaki, Yasaman
Peng, Hua
Xiang, Chun-Lei
Leveraging plastomes for comparative analysis and phylogenomic inference within Scutellarioideae (Lamiaceae)
title Leveraging plastomes for comparative analysis and phylogenomic inference within Scutellarioideae (Lamiaceae)
title_full Leveraging plastomes for comparative analysis and phylogenomic inference within Scutellarioideae (Lamiaceae)
title_fullStr Leveraging plastomes for comparative analysis and phylogenomic inference within Scutellarioideae (Lamiaceae)
title_full_unstemmed Leveraging plastomes for comparative analysis and phylogenomic inference within Scutellarioideae (Lamiaceae)
title_short Leveraging plastomes for comparative analysis and phylogenomic inference within Scutellarioideae (Lamiaceae)
title_sort leveraging plastomes for comparative analysis and phylogenomic inference within scutellarioideae (lamiaceae)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7205251/
https://www.ncbi.nlm.nih.gov/pubmed/32379799
http://dx.doi.org/10.1371/journal.pone.0232602
work_keys_str_mv AT zhaofei leveragingplastomesforcomparativeanalysisandphylogenomicinferencewithinscutellarioideaelamiaceae
AT libo leveragingplastomesforcomparativeanalysisandphylogenomicinferencewithinscutellarioideaelamiaceae
AT drewbryant leveragingplastomesforcomparativeanalysisandphylogenomicinferencewithinscutellarioideaelamiaceae
AT chenyaping leveragingplastomesforcomparativeanalysisandphylogenomicinferencewithinscutellarioideaelamiaceae
AT wangqiang leveragingplastomesforcomparativeanalysisandphylogenomicinferencewithinscutellarioideaelamiaceae
AT yuwenbin leveragingplastomesforcomparativeanalysisandphylogenomicinferencewithinscutellarioideaelamiaceae
AT liuende leveragingplastomesforcomparativeanalysisandphylogenomicinferencewithinscutellarioideaelamiaceae
AT salmakiyasaman leveragingplastomesforcomparativeanalysisandphylogenomicinferencewithinscutellarioideaelamiaceae
AT penghua leveragingplastomesforcomparativeanalysisandphylogenomicinferencewithinscutellarioideaelamiaceae
AT xiangchunlei leveragingplastomesforcomparativeanalysisandphylogenomicinferencewithinscutellarioideaelamiaceae