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Kethoxal-assisted single-stranded DNA sequencing captures global transcription dynamics and enhancer activity in situ

Transcription is a highly dynamic process that generates single-stranded DNA (ssDNA) in the genome as ‘transcription bubbles’. Here we describe a kethoxal-assisted single-stranded DNA sequencing (KAS-seq) approach, based on the fast and specific reaction between N(3)-kethoxal and guanines in ssDNA i...

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Detalles Bibliográficos
Autores principales:	Wu, Tong, Lyu, Ruitu, You, Qiancheng, He, Chuan
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	2020
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7205578/ https://www.ncbi.nlm.nih.gov/pubmed/32251394 http://dx.doi.org/10.1038/s41592-020-0797-9

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