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The lncRNA PVT1 regulates nasopharyngeal carcinoma cell proliferation via activating the KAT2A acetyltransferase and stabilizing HIF-1α

Long noncoding RNAs (lncRNAs) play important roles in regulating the development and progression of many cancers. However, the clinical significance of specific lncRNAs in the context of nasopharyngeal carcinoma (NPC) and the molecular mechanisms by which they regulate this form of cancer remain lar...

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Autores principales: Wang, Ying, Chen, Wanyuan, Lian, Jiayan, Zhang, Haibo, Yu, Bo, Zhang, Minjun, Wei, Fangqiang, Wu, Jianhui, Jiang, Jiaxiang, Jia, Yongshi, Mo, Fan, zhang, Shirong, Liang, Xiaodong, Mou, Xiaozhou, Tang, Jianming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7206084/
https://www.ncbi.nlm.nih.gov/pubmed/31320749
http://dx.doi.org/10.1038/s41418-019-0381-y
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author Wang, Ying
Chen, Wanyuan
Lian, Jiayan
Zhang, Haibo
Yu, Bo
Zhang, Minjun
Wei, Fangqiang
Wu, Jianhui
Jiang, Jiaxiang
Jia, Yongshi
Mo, Fan
zhang, Shirong
Liang, Xiaodong
Mou, Xiaozhou
Tang, Jianming
author_facet Wang, Ying
Chen, Wanyuan
Lian, Jiayan
Zhang, Haibo
Yu, Bo
Zhang, Minjun
Wei, Fangqiang
Wu, Jianhui
Jiang, Jiaxiang
Jia, Yongshi
Mo, Fan
zhang, Shirong
Liang, Xiaodong
Mou, Xiaozhou
Tang, Jianming
author_sort Wang, Ying
collection PubMed
description Long noncoding RNAs (lncRNAs) play important roles in regulating the development and progression of many cancers. However, the clinical significance of specific lncRNAs in the context of nasopharyngeal carcinoma (NPC) and the molecular mechanisms by which they regulate this form of cancer remain largely unclear. In this study we found that the lncRNA PVT1 was upregulated in NPC, and that in patients this upregulation was associated with reduced survival. RNA sequencing revealed that PVT1 was responsible for regulating NPC cell proliferation and for controlling a hypoxia-related phenotype in these cells. PVT1 knockdown reduced NPC cell proliferation, colony formation, and tumorigenesis in a subcutaneous mouse xenograft model systems. We further found that PVT1 serves as a scaffold for the chromatin modification factor KAT2A, which mediates histone 3 lysine 9 acetylation (H3K9), recruiting the nuclear receptor binding protein TIF1β to activate NF90 transcription, thereby increasing HIF-1α stability and promoting a malignant phenotype in NPC cells. Overexpression of NF90 or HIF-1α restored the proliferation in cells that had ceased proliferating due to PVT1 or KAT2A depletion. Conversely, overexpression of active KAT2A or TIF1β, but not of KAT2A acetyltransferase activity-deficient mutants or TIF1β isoforms lacking H3K9ac binding sites, promoted a PVT1-mediated increase in NF90 transcription, as well as increased HIF-1α stability and cell proliferation. PVT1 knockdown enhanced the radiosensitization effect in NPC cells via inhibiting binding between H3K9ac and TIF1β in a manner. Taken together, our results demonstrate that PVT1 serves an oncogenic role and plays an important role in radiosensitivity in malignant NPC via activating the KAT2A acetyltransferase and stabilizing HIF-1α.
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spelling pubmed-72060842020-05-08 The lncRNA PVT1 regulates nasopharyngeal carcinoma cell proliferation via activating the KAT2A acetyltransferase and stabilizing HIF-1α Wang, Ying Chen, Wanyuan Lian, Jiayan Zhang, Haibo Yu, Bo Zhang, Minjun Wei, Fangqiang Wu, Jianhui Jiang, Jiaxiang Jia, Yongshi Mo, Fan zhang, Shirong Liang, Xiaodong Mou, Xiaozhou Tang, Jianming Cell Death Differ Article Long noncoding RNAs (lncRNAs) play important roles in regulating the development and progression of many cancers. However, the clinical significance of specific lncRNAs in the context of nasopharyngeal carcinoma (NPC) and the molecular mechanisms by which they regulate this form of cancer remain largely unclear. In this study we found that the lncRNA PVT1 was upregulated in NPC, and that in patients this upregulation was associated with reduced survival. RNA sequencing revealed that PVT1 was responsible for regulating NPC cell proliferation and for controlling a hypoxia-related phenotype in these cells. PVT1 knockdown reduced NPC cell proliferation, colony formation, and tumorigenesis in a subcutaneous mouse xenograft model systems. We further found that PVT1 serves as a scaffold for the chromatin modification factor KAT2A, which mediates histone 3 lysine 9 acetylation (H3K9), recruiting the nuclear receptor binding protein TIF1β to activate NF90 transcription, thereby increasing HIF-1α stability and promoting a malignant phenotype in NPC cells. Overexpression of NF90 or HIF-1α restored the proliferation in cells that had ceased proliferating due to PVT1 or KAT2A depletion. Conversely, overexpression of active KAT2A or TIF1β, but not of KAT2A acetyltransferase activity-deficient mutants or TIF1β isoforms lacking H3K9ac binding sites, promoted a PVT1-mediated increase in NF90 transcription, as well as increased HIF-1α stability and cell proliferation. PVT1 knockdown enhanced the radiosensitization effect in NPC cells via inhibiting binding between H3K9ac and TIF1β in a manner. Taken together, our results demonstrate that PVT1 serves an oncogenic role and plays an important role in radiosensitivity in malignant NPC via activating the KAT2A acetyltransferase and stabilizing HIF-1α. Nature Publishing Group UK 2019-07-18 2020-02 /pmc/articles/PMC7206084/ /pubmed/31320749 http://dx.doi.org/10.1038/s41418-019-0381-y Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Wang, Ying
Chen, Wanyuan
Lian, Jiayan
Zhang, Haibo
Yu, Bo
Zhang, Minjun
Wei, Fangqiang
Wu, Jianhui
Jiang, Jiaxiang
Jia, Yongshi
Mo, Fan
zhang, Shirong
Liang, Xiaodong
Mou, Xiaozhou
Tang, Jianming
The lncRNA PVT1 regulates nasopharyngeal carcinoma cell proliferation via activating the KAT2A acetyltransferase and stabilizing HIF-1α
title The lncRNA PVT1 regulates nasopharyngeal carcinoma cell proliferation via activating the KAT2A acetyltransferase and stabilizing HIF-1α
title_full The lncRNA PVT1 regulates nasopharyngeal carcinoma cell proliferation via activating the KAT2A acetyltransferase and stabilizing HIF-1α
title_fullStr The lncRNA PVT1 regulates nasopharyngeal carcinoma cell proliferation via activating the KAT2A acetyltransferase and stabilizing HIF-1α
title_full_unstemmed The lncRNA PVT1 regulates nasopharyngeal carcinoma cell proliferation via activating the KAT2A acetyltransferase and stabilizing HIF-1α
title_short The lncRNA PVT1 regulates nasopharyngeal carcinoma cell proliferation via activating the KAT2A acetyltransferase and stabilizing HIF-1α
title_sort lncrna pvt1 regulates nasopharyngeal carcinoma cell proliferation via activating the kat2a acetyltransferase and stabilizing hif-1α
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7206084/
https://www.ncbi.nlm.nih.gov/pubmed/31320749
http://dx.doi.org/10.1038/s41418-019-0381-y
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