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Negative depletion mediated brightfield circulating tumour cell identification strategy on microparticle-based microfluidic chip

BACKGROUND: The most convenient circulating tumor cells (CTCs) identification method is direct analysis of cells under bright field microscopy by which CTCs can be comprehensive studied based on morphology, phenotype or even cellular function. However, universal cell markers and a standard tumour ce...

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Autores principales: Wang, Shuibing, Hong, Shaoli, Cai, Shijia, Lei, Jia, Chen, Jinyao, Zhang, Nangang, Ai, Zhao, Liu, Kan, Tang, Man
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7206695/
https://www.ncbi.nlm.nih.gov/pubmed/32381091
http://dx.doi.org/10.1186/s12951-020-00623-4
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author Wang, Shuibing
Hong, Shaoli
Cai, Shijia
Lei, Jia
Chen, Jinyao
Zhang, Nangang
Ai, Zhao
Liu, Kan
Tang, Man
author_facet Wang, Shuibing
Hong, Shaoli
Cai, Shijia
Lei, Jia
Chen, Jinyao
Zhang, Nangang
Ai, Zhao
Liu, Kan
Tang, Man
author_sort Wang, Shuibing
collection PubMed
description BACKGROUND: The most convenient circulating tumor cells (CTCs) identification method is direct analysis of cells under bright field microscopy by which CTCs can be comprehensive studied based on morphology, phenotype or even cellular function. However, universal cell markers and a standard tumour cell map do not exist, thus limiting the clinical application of CTCs. RESULTS: This paper focuses on an automatic and convenient negative depletion strategy for circulating tumour cell identification under bright field microscopy. In this strategy, immune microparticles (IMPs) are applied to negatively label white blood cells rather than the tumour cells, such that tumour cells can be directly distinguished under brightfield of the microscopy. In this way, all of the heterogeneous tumour cells and their phenotype properties can be retained for further cancer-related studies. In addition, a wedge-shaped microfluidic chip is constructed for heterogeneous CTC pre-purification and enrichment by size, thus significantly decreasing the interference of haematological cells. Additionally, all cell treatments are processed automatically, and the tumour cells can be rapidly counted and distinguished via customized cell analytical software, showing high detection efficiency and automation. This IMPs based negative cell labelling strategy can also be combined with other classic cell identification methods, thus demonstrating its excellent compatibility. CONCLUSION: This identification strategy features simple and harmless for tumour cells, as well as excellent accuracy and efficiency. And the low equipment demand and high automation level make it promise for extensive application in basic medical institutions.
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spelling pubmed-72066952020-05-14 Negative depletion mediated brightfield circulating tumour cell identification strategy on microparticle-based microfluidic chip Wang, Shuibing Hong, Shaoli Cai, Shijia Lei, Jia Chen, Jinyao Zhang, Nangang Ai, Zhao Liu, Kan Tang, Man J Nanobiotechnology Research BACKGROUND: The most convenient circulating tumor cells (CTCs) identification method is direct analysis of cells under bright field microscopy by which CTCs can be comprehensive studied based on morphology, phenotype or even cellular function. However, universal cell markers and a standard tumour cell map do not exist, thus limiting the clinical application of CTCs. RESULTS: This paper focuses on an automatic and convenient negative depletion strategy for circulating tumour cell identification under bright field microscopy. In this strategy, immune microparticles (IMPs) are applied to negatively label white blood cells rather than the tumour cells, such that tumour cells can be directly distinguished under brightfield of the microscopy. In this way, all of the heterogeneous tumour cells and their phenotype properties can be retained for further cancer-related studies. In addition, a wedge-shaped microfluidic chip is constructed for heterogeneous CTC pre-purification and enrichment by size, thus significantly decreasing the interference of haematological cells. Additionally, all cell treatments are processed automatically, and the tumour cells can be rapidly counted and distinguished via customized cell analytical software, showing high detection efficiency and automation. This IMPs based negative cell labelling strategy can also be combined with other classic cell identification methods, thus demonstrating its excellent compatibility. CONCLUSION: This identification strategy features simple and harmless for tumour cells, as well as excellent accuracy and efficiency. And the low equipment demand and high automation level make it promise for extensive application in basic medical institutions. BioMed Central 2020-05-07 /pmc/articles/PMC7206695/ /pubmed/32381091 http://dx.doi.org/10.1186/s12951-020-00623-4 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Wang, Shuibing
Hong, Shaoli
Cai, Shijia
Lei, Jia
Chen, Jinyao
Zhang, Nangang
Ai, Zhao
Liu, Kan
Tang, Man
Negative depletion mediated brightfield circulating tumour cell identification strategy on microparticle-based microfluidic chip
title Negative depletion mediated brightfield circulating tumour cell identification strategy on microparticle-based microfluidic chip
title_full Negative depletion mediated brightfield circulating tumour cell identification strategy on microparticle-based microfluidic chip
title_fullStr Negative depletion mediated brightfield circulating tumour cell identification strategy on microparticle-based microfluidic chip
title_full_unstemmed Negative depletion mediated brightfield circulating tumour cell identification strategy on microparticle-based microfluidic chip
title_short Negative depletion mediated brightfield circulating tumour cell identification strategy on microparticle-based microfluidic chip
title_sort negative depletion mediated brightfield circulating tumour cell identification strategy on microparticle-based microfluidic chip
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7206695/
https://www.ncbi.nlm.nih.gov/pubmed/32381091
http://dx.doi.org/10.1186/s12951-020-00623-4
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