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Molecular surveillance of the Pfmdr1 N86Y allele among Congolese pregnant women with asymptomatic malaria
BACKGROUND: Malaria in pregnancy is associated with considerable morbidity and mortality. Regular surveillance of artemisinin-based combination therapy tolerance, or molecular makers of resistance, is vital for effective malaria treatment, control and eradication programmes. Plasmodium falciparum mu...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7206803/ https://www.ncbi.nlm.nih.gov/pubmed/32384930 http://dx.doi.org/10.1186/s12936-020-03246-0 |
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author | Dossou-Yovo, Louis Regis Ntoumi, Francine Koukouikila-Koussounda, Felix Vouvoungui, Jeannhey Christevy Adedoja, Ayodele Nderu, David Velavan, Thirumalaisamy P. Lenga, Arsène |
author_facet | Dossou-Yovo, Louis Regis Ntoumi, Francine Koukouikila-Koussounda, Felix Vouvoungui, Jeannhey Christevy Adedoja, Ayodele Nderu, David Velavan, Thirumalaisamy P. Lenga, Arsène |
author_sort | Dossou-Yovo, Louis Regis |
collection | PubMed |
description | BACKGROUND: Malaria in pregnancy is associated with considerable morbidity and mortality. Regular surveillance of artemisinin-based combination therapy tolerance, or molecular makers of resistance, is vital for effective malaria treatment, control and eradication programmes. Plasmodium falciparum multiple drug resistance-1 gene (Pfmdr1) N86Y mutation is associated with reduced susceptibility to lumefantrine. This study assessed the prevalence of Pfmdr1 N86Y in Brazzaville, Republic of Congo. METHODS: A total 1001 of P. falciparum-infected blood samples obtained from asymptomatic malaria pregnant women having a normal child delivery at the Madibou Integrated Health Centre were analysed. Pfmdr1 N86Y genotyping was conducted using PCR-restriction fragment length polymorphism. RESULTS: The wild type Pfmdr1 N86 allele was predominant (> 68%) in this study, whereas a few isolates carrying the either the mutant allele (Pfmdr1 86Y) alone or both alleles (mixed genotype). The dominance of the wildtype allele (pfmdr1 N86) indicates the plausible decline P. falciparum susceptibility to lumefantrine. CONCLUSION: This study gives an update on the prevalence of Pfmdr1 N86Y alleles in Brazzaville, Republic of Congo. It also raises concern on the imminent emergence of resistance against artemether–lumefantrine in this setting. This study underscores the importance to regular artemether–lumefantrine efficacy monitoring to inform the malaria control programme of the Republic of Congo. |
format | Online Article Text |
id | pubmed-7206803 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-72068032020-05-15 Molecular surveillance of the Pfmdr1 N86Y allele among Congolese pregnant women with asymptomatic malaria Dossou-Yovo, Louis Regis Ntoumi, Francine Koukouikila-Koussounda, Felix Vouvoungui, Jeannhey Christevy Adedoja, Ayodele Nderu, David Velavan, Thirumalaisamy P. Lenga, Arsène Malar J Research BACKGROUND: Malaria in pregnancy is associated with considerable morbidity and mortality. Regular surveillance of artemisinin-based combination therapy tolerance, or molecular makers of resistance, is vital for effective malaria treatment, control and eradication programmes. Plasmodium falciparum multiple drug resistance-1 gene (Pfmdr1) N86Y mutation is associated with reduced susceptibility to lumefantrine. This study assessed the prevalence of Pfmdr1 N86Y in Brazzaville, Republic of Congo. METHODS: A total 1001 of P. falciparum-infected blood samples obtained from asymptomatic malaria pregnant women having a normal child delivery at the Madibou Integrated Health Centre were analysed. Pfmdr1 N86Y genotyping was conducted using PCR-restriction fragment length polymorphism. RESULTS: The wild type Pfmdr1 N86 allele was predominant (> 68%) in this study, whereas a few isolates carrying the either the mutant allele (Pfmdr1 86Y) alone or both alleles (mixed genotype). The dominance of the wildtype allele (pfmdr1 N86) indicates the plausible decline P. falciparum susceptibility to lumefantrine. CONCLUSION: This study gives an update on the prevalence of Pfmdr1 N86Y alleles in Brazzaville, Republic of Congo. It also raises concern on the imminent emergence of resistance against artemether–lumefantrine in this setting. This study underscores the importance to regular artemether–lumefantrine efficacy monitoring to inform the malaria control programme of the Republic of Congo. BioMed Central 2020-05-08 /pmc/articles/PMC7206803/ /pubmed/32384930 http://dx.doi.org/10.1186/s12936-020-03246-0 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Dossou-Yovo, Louis Regis Ntoumi, Francine Koukouikila-Koussounda, Felix Vouvoungui, Jeannhey Christevy Adedoja, Ayodele Nderu, David Velavan, Thirumalaisamy P. Lenga, Arsène Molecular surveillance of the Pfmdr1 N86Y allele among Congolese pregnant women with asymptomatic malaria |
title | Molecular surveillance of the Pfmdr1 N86Y allele among Congolese pregnant women with asymptomatic malaria |
title_full | Molecular surveillance of the Pfmdr1 N86Y allele among Congolese pregnant women with asymptomatic malaria |
title_fullStr | Molecular surveillance of the Pfmdr1 N86Y allele among Congolese pregnant women with asymptomatic malaria |
title_full_unstemmed | Molecular surveillance of the Pfmdr1 N86Y allele among Congolese pregnant women with asymptomatic malaria |
title_short | Molecular surveillance of the Pfmdr1 N86Y allele among Congolese pregnant women with asymptomatic malaria |
title_sort | molecular surveillance of the pfmdr1 n86y allele among congolese pregnant women with asymptomatic malaria |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7206803/ https://www.ncbi.nlm.nih.gov/pubmed/32384930 http://dx.doi.org/10.1186/s12936-020-03246-0 |
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