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Effects of coal-fired PM(2.5) on the expression levels of atherosclerosis-related proteins and the phosphorylation level of MAPK in ApoE(−/−) mice

BACKGROUND: Air pollution increases the morbidity and mortality of cardiovascular disease (CVD). Atherosclerosis (AS) is the pathological basis of most CVD, and the progression of atherosclerosis and the increase of fragile plaque rupture are the mechanism basis of the relationship between atmospher...

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Autores principales: Wang, Siqi, Wang, Feifei, Yang, Lixin, Li, Qin, Huang, Yao, Cheng, Zhiyuan, Chu, Hongqian, Song, Yiming, Shang, Lanqin, Hao, Weidong, Wei, Xuetao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7206822/
https://www.ncbi.nlm.nih.gov/pubmed/32384920
http://dx.doi.org/10.1186/s40360-020-00411-8
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author Wang, Siqi
Wang, Feifei
Yang, Lixin
Li, Qin
Huang, Yao
Cheng, Zhiyuan
Chu, Hongqian
Song, Yiming
Shang, Lanqin
Hao, Weidong
Wei, Xuetao
author_facet Wang, Siqi
Wang, Feifei
Yang, Lixin
Li, Qin
Huang, Yao
Cheng, Zhiyuan
Chu, Hongqian
Song, Yiming
Shang, Lanqin
Hao, Weidong
Wei, Xuetao
author_sort Wang, Siqi
collection PubMed
description BACKGROUND: Air pollution increases the morbidity and mortality of cardiovascular disease (CVD). Atherosclerosis (AS) is the pathological basis of most CVD, and the progression of atherosclerosis and the increase of fragile plaque rupture are the mechanism basis of the relationship between atmospheric particulate pollution and CVD. The aim of the present study was to investigate the effects of coal-fired fine particulate matter (PM(2.5)) on the expression levels of atherosclerosis-related proteins (von Willebrand factor (vWF), Endothelin-1 (ET-1), intercellular adhesion molecule-1 (ICAM-1), and E-selectin, and to explore the role and mechanism of the progression of atherosclerosis induced by coal-fired PM(2.5) via the mitogen-activated protein kinase (MAPK) signaling pathways. METHODS: Different concentrations of PM(2.5) were given to apolipoprotein-E knockout (ApoE(−/−)) mice via intratracheal instillation for 8 weeks. Enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of vWF, ET-1 in serum of mice. Immunohistochemistry was used to observe the expression and distribution of ICAM-1 and E-selectin in the aorta of mice. Western blot was used to investigate the phosphoylation of proteins relevant to MAPK signaling pathways. RESULTS: Coal-fired PM(2.5) exacerbated atherosclerosis induced by a high-fat diet. Fibrous cap formation, foam cells accumulation, and atherosclerotic lesions were observed in the aortas of PM(2.5)-treated mice. Coal-fired PM(2.5) increased the protein levels of ET-1, ICAM-1, and E-selectin, but there was no significant difference in the vWF levels between the PM(2.5)-treatment mice and the HFD control mice. Coal-fired PM(2.5) promoted the phosphorylation of p38, c-Jun N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK) in aortic tissues of mice. CONCLUSION: Coal-derived PM(2.5) exacerbated the formation of atherosclerosis in mice, increased the expression levels of atherosclerosis-related proteins in mice serum, and promoted the phosphorylation of proteins relevant to MAPK signaling pathway. Thus, MAPK signaling pathway may play a role in the atherosclerosis pathogenesis induced by Coal-derived PM(2.5).
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spelling pubmed-72068222020-05-15 Effects of coal-fired PM(2.5) on the expression levels of atherosclerosis-related proteins and the phosphorylation level of MAPK in ApoE(−/−) mice Wang, Siqi Wang, Feifei Yang, Lixin Li, Qin Huang, Yao Cheng, Zhiyuan Chu, Hongqian Song, Yiming Shang, Lanqin Hao, Weidong Wei, Xuetao BMC Pharmacol Toxicol Research Article BACKGROUND: Air pollution increases the morbidity and mortality of cardiovascular disease (CVD). Atherosclerosis (AS) is the pathological basis of most CVD, and the progression of atherosclerosis and the increase of fragile plaque rupture are the mechanism basis of the relationship between atmospheric particulate pollution and CVD. The aim of the present study was to investigate the effects of coal-fired fine particulate matter (PM(2.5)) on the expression levels of atherosclerosis-related proteins (von Willebrand factor (vWF), Endothelin-1 (ET-1), intercellular adhesion molecule-1 (ICAM-1), and E-selectin, and to explore the role and mechanism of the progression of atherosclerosis induced by coal-fired PM(2.5) via the mitogen-activated protein kinase (MAPK) signaling pathways. METHODS: Different concentrations of PM(2.5) were given to apolipoprotein-E knockout (ApoE(−/−)) mice via intratracheal instillation for 8 weeks. Enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of vWF, ET-1 in serum of mice. Immunohistochemistry was used to observe the expression and distribution of ICAM-1 and E-selectin in the aorta of mice. Western blot was used to investigate the phosphoylation of proteins relevant to MAPK signaling pathways. RESULTS: Coal-fired PM(2.5) exacerbated atherosclerosis induced by a high-fat diet. Fibrous cap formation, foam cells accumulation, and atherosclerotic lesions were observed in the aortas of PM(2.5)-treated mice. Coal-fired PM(2.5) increased the protein levels of ET-1, ICAM-1, and E-selectin, but there was no significant difference in the vWF levels between the PM(2.5)-treatment mice and the HFD control mice. Coal-fired PM(2.5) promoted the phosphorylation of p38, c-Jun N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK) in aortic tissues of mice. CONCLUSION: Coal-derived PM(2.5) exacerbated the formation of atherosclerosis in mice, increased the expression levels of atherosclerosis-related proteins in mice serum, and promoted the phosphorylation of proteins relevant to MAPK signaling pathway. Thus, MAPK signaling pathway may play a role in the atherosclerosis pathogenesis induced by Coal-derived PM(2.5). BioMed Central 2020-05-08 /pmc/articles/PMC7206822/ /pubmed/32384920 http://dx.doi.org/10.1186/s40360-020-00411-8 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Wang, Siqi
Wang, Feifei
Yang, Lixin
Li, Qin
Huang, Yao
Cheng, Zhiyuan
Chu, Hongqian
Song, Yiming
Shang, Lanqin
Hao, Weidong
Wei, Xuetao
Effects of coal-fired PM(2.5) on the expression levels of atherosclerosis-related proteins and the phosphorylation level of MAPK in ApoE(−/−) mice
title Effects of coal-fired PM(2.5) on the expression levels of atherosclerosis-related proteins and the phosphorylation level of MAPK in ApoE(−/−) mice
title_full Effects of coal-fired PM(2.5) on the expression levels of atherosclerosis-related proteins and the phosphorylation level of MAPK in ApoE(−/−) mice
title_fullStr Effects of coal-fired PM(2.5) on the expression levels of atherosclerosis-related proteins and the phosphorylation level of MAPK in ApoE(−/−) mice
title_full_unstemmed Effects of coal-fired PM(2.5) on the expression levels of atherosclerosis-related proteins and the phosphorylation level of MAPK in ApoE(−/−) mice
title_short Effects of coal-fired PM(2.5) on the expression levels of atherosclerosis-related proteins and the phosphorylation level of MAPK in ApoE(−/−) mice
title_sort effects of coal-fired pm(2.5) on the expression levels of atherosclerosis-related proteins and the phosphorylation level of mapk in apoe(−/−) mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7206822/
https://www.ncbi.nlm.nih.gov/pubmed/32384920
http://dx.doi.org/10.1186/s40360-020-00411-8
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