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Combined therapy of photobiomodulation and adipose-derived stem cells synergistically improve healing in an ischemic, infected and delayed healing wound model in rats with type 1 diabetes mellitus
OBJECTIVE: We assessed the therapeutic effects of photobiomodulation (PBM) and adipose-derived stem cell (ADS) treatments individually and together on the maturation step of repairing of a delayed healing wound model in rats with type 1 diabetes mellitus (DM1). RESEARCH DESIGN AND METHODS: We random...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BMJ Publishing Group
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7206914/ https://www.ncbi.nlm.nih.gov/pubmed/32098898 http://dx.doi.org/10.1136/bmjdrc-2019-001033 |
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author | Ebrahimpour-Malekshah, Roohollah Amini, Abdollah Zare, Fatemeh Mostafavinia, Atarodsadat Davoody, Samin Deravi, Niloofar Rahmanian, Mohammad Hashemi, Seyed Mahmoud Habibi, Malihe Ghoreishi, Seyed Kamran Chien, Sufan Shafikhani, Sasha Ahmadi, Houssein Bayat, Sahar Bayat, Mohammad |
author_facet | Ebrahimpour-Malekshah, Roohollah Amini, Abdollah Zare, Fatemeh Mostafavinia, Atarodsadat Davoody, Samin Deravi, Niloofar Rahmanian, Mohammad Hashemi, Seyed Mahmoud Habibi, Malihe Ghoreishi, Seyed Kamran Chien, Sufan Shafikhani, Sasha Ahmadi, Houssein Bayat, Sahar Bayat, Mohammad |
author_sort | Ebrahimpour-Malekshah, Roohollah |
collection | PubMed |
description | OBJECTIVE: We assessed the therapeutic effects of photobiomodulation (PBM) and adipose-derived stem cell (ADS) treatments individually and together on the maturation step of repairing of a delayed healing wound model in rats with type 1 diabetes mellitus (DM1). RESEARCH DESIGN AND METHODS: We randomly assigned 24 rats with DM1 to four groups (n=6 per group). Group 1 was the control (placebo) group. In group 2, allograft human ADSs were transplanted. Group 3 was subjected to PBM (wavelength: 890 nm, peak power output: 80 W, pulse frequency: 80 Hz, pulsed duration: 180 ns, duration of exposure for each point: 200 s, power density: 0.001 W/cm(2), energy density: 0.2 J/cm(2)) immediately after surgery, which continued for 6 days per week for 16 days. Group 4 received both the human ADS and PBM. In addition, we inflicted an ischemic, delayed healing, and infected wound simulation in all of the rats. The wounds were infected with methicillin-resistant Staphylococcus aureus (MRSA). RESULTS: All three treatment regimens significantly decreased the amount of microbial flora, significantly increased wound strength and significantly modulated inflammatory response and significantly increased angiogenesis on day 16. Microbiological analysis showed that PBM+ADS was significantly better than PBM and ADS alone. In terms of wound closure rate and angiogenesis, PBM+ADS was significantly better than the PBM, ADS and control groups. CONCLUSIONS: Combination therapy of PBM+ADS is more effective that either PBM or ADS in stimulating skin injury repair, and modulating inflammatory response in an MRSA-infected wound model of rats with DM1. |
format | Online Article Text |
id | pubmed-7206914 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BMJ Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-72069142020-05-12 Combined therapy of photobiomodulation and adipose-derived stem cells synergistically improve healing in an ischemic, infected and delayed healing wound model in rats with type 1 diabetes mellitus Ebrahimpour-Malekshah, Roohollah Amini, Abdollah Zare, Fatemeh Mostafavinia, Atarodsadat Davoody, Samin Deravi, Niloofar Rahmanian, Mohammad Hashemi, Seyed Mahmoud Habibi, Malihe Ghoreishi, Seyed Kamran Chien, Sufan Shafikhani, Sasha Ahmadi, Houssein Bayat, Sahar Bayat, Mohammad BMJ Open Diabetes Res Care Clinical Care/Education/Nutrition OBJECTIVE: We assessed the therapeutic effects of photobiomodulation (PBM) and adipose-derived stem cell (ADS) treatments individually and together on the maturation step of repairing of a delayed healing wound model in rats with type 1 diabetes mellitus (DM1). RESEARCH DESIGN AND METHODS: We randomly assigned 24 rats with DM1 to four groups (n=6 per group). Group 1 was the control (placebo) group. In group 2, allograft human ADSs were transplanted. Group 3 was subjected to PBM (wavelength: 890 nm, peak power output: 80 W, pulse frequency: 80 Hz, pulsed duration: 180 ns, duration of exposure for each point: 200 s, power density: 0.001 W/cm(2), energy density: 0.2 J/cm(2)) immediately after surgery, which continued for 6 days per week for 16 days. Group 4 received both the human ADS and PBM. In addition, we inflicted an ischemic, delayed healing, and infected wound simulation in all of the rats. The wounds were infected with methicillin-resistant Staphylococcus aureus (MRSA). RESULTS: All three treatment regimens significantly decreased the amount of microbial flora, significantly increased wound strength and significantly modulated inflammatory response and significantly increased angiogenesis on day 16. Microbiological analysis showed that PBM+ADS was significantly better than PBM and ADS alone. In terms of wound closure rate and angiogenesis, PBM+ADS was significantly better than the PBM, ADS and control groups. CONCLUSIONS: Combination therapy of PBM+ADS is more effective that either PBM or ADS in stimulating skin injury repair, and modulating inflammatory response in an MRSA-infected wound model of rats with DM1. BMJ Publishing Group 2020-02-24 /pmc/articles/PMC7206914/ /pubmed/32098898 http://dx.doi.org/10.1136/bmjdrc-2019-001033 Text en © Author(s) (or their employer(s)) 2020. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) . |
spellingShingle | Clinical Care/Education/Nutrition Ebrahimpour-Malekshah, Roohollah Amini, Abdollah Zare, Fatemeh Mostafavinia, Atarodsadat Davoody, Samin Deravi, Niloofar Rahmanian, Mohammad Hashemi, Seyed Mahmoud Habibi, Malihe Ghoreishi, Seyed Kamran Chien, Sufan Shafikhani, Sasha Ahmadi, Houssein Bayat, Sahar Bayat, Mohammad Combined therapy of photobiomodulation and adipose-derived stem cells synergistically improve healing in an ischemic, infected and delayed healing wound model in rats with type 1 diabetes mellitus |
title | Combined therapy of photobiomodulation and adipose-derived stem cells synergistically improve healing in an ischemic, infected and delayed healing wound model in rats with type 1 diabetes mellitus |
title_full | Combined therapy of photobiomodulation and adipose-derived stem cells synergistically improve healing in an ischemic, infected and delayed healing wound model in rats with type 1 diabetes mellitus |
title_fullStr | Combined therapy of photobiomodulation and adipose-derived stem cells synergistically improve healing in an ischemic, infected and delayed healing wound model in rats with type 1 diabetes mellitus |
title_full_unstemmed | Combined therapy of photobiomodulation and adipose-derived stem cells synergistically improve healing in an ischemic, infected and delayed healing wound model in rats with type 1 diabetes mellitus |
title_short | Combined therapy of photobiomodulation and adipose-derived stem cells synergistically improve healing in an ischemic, infected and delayed healing wound model in rats with type 1 diabetes mellitus |
title_sort | combined therapy of photobiomodulation and adipose-derived stem cells synergistically improve healing in an ischemic, infected and delayed healing wound model in rats with type 1 diabetes mellitus |
topic | Clinical Care/Education/Nutrition |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7206914/ https://www.ncbi.nlm.nih.gov/pubmed/32098898 http://dx.doi.org/10.1136/bmjdrc-2019-001033 |
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