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SAT-136 Hypoxia Effect on Cytokine Production by Breast Cancer Cells

Inflammation is a critical component of tumor initiation and progression. Chronic inflammation triggers molecular events that can promote carcinogenesis, tumor vascularization and metastasis. As inflammatory mediators, cytokines play an important role in the interplay between the tumor cells and tum...

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Autores principales: Avtanski, Dimiter, Chen, Karin, Satlof, Leo, Stoffels, Guillaume, Kothapalli, Udithi, Ziluck, Noah, Lema, Maribel, Poretsky, Leonid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7207306/
http://dx.doi.org/10.1210/jendso/bvaa046.1328
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author Avtanski, Dimiter
Chen, Karin
Satlof, Leo
Stoffels, Guillaume
Kothapalli, Udithi
Ziluck, Noah
Lema, Maribel
Poretsky, Leonid
author_facet Avtanski, Dimiter
Chen, Karin
Satlof, Leo
Stoffels, Guillaume
Kothapalli, Udithi
Ziluck, Noah
Lema, Maribel
Poretsky, Leonid
author_sort Avtanski, Dimiter
collection PubMed
description Inflammation is a critical component of tumor initiation and progression. Chronic inflammation triggers molecular events that can promote carcinogenesis, tumor vascularization and metastasis. As inflammatory mediators, cytokines play an important role in the interplay between the tumor cells and tumor microenvironment. Cytokines released by the tumor-associated macrophages modulate cancer cell survival, stemness, invasiveness, and tumor vascularization. Breast cancer cells, however, also produce a variety of cytokines, whose role in cancer development is poorly understood. The aim of our study was to characterize the basal cytokine secretory activity in commonly used human breast cancer cell lines (MDA-MB-231, MCF-7, BT-474, and T-47D). Using MILLIPLEX assay, we measured the expression of 41 cytokines, including interleukins, monokines, interferons and growth factors. We also compared cytokine expression profile of breast cancer cells with those of non-tumorigenic human breast epithelial MCF-10A cells. Further, we investigated whether hypoxia modulates cytokine secretion of breast cancer cells. Using cobalt(II) chloride (CoCl(2)) to mimic hypoxia, we compared the effect of various treatment doses and intervals on cytokine production in the breast cancer cells. Results demonstrated that CoCl(2) affects the release of multiple cytokines in a dose- and concentration-dependent manner, thus highlighting the role of cancer cell-derived cytokines on breast tumor progression. Understanding the molecular actions of cytokines in the tumor microenvironment is important for understanding the mechanism of cancer initiation and progression.
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spelling pubmed-72073062020-05-12 SAT-136 Hypoxia Effect on Cytokine Production by Breast Cancer Cells Avtanski, Dimiter Chen, Karin Satlof, Leo Stoffels, Guillaume Kothapalli, Udithi Ziluck, Noah Lema, Maribel Poretsky, Leonid J Endocr Soc Tumor Biology Inflammation is a critical component of tumor initiation and progression. Chronic inflammation triggers molecular events that can promote carcinogenesis, tumor vascularization and metastasis. As inflammatory mediators, cytokines play an important role in the interplay between the tumor cells and tumor microenvironment. Cytokines released by the tumor-associated macrophages modulate cancer cell survival, stemness, invasiveness, and tumor vascularization. Breast cancer cells, however, also produce a variety of cytokines, whose role in cancer development is poorly understood. The aim of our study was to characterize the basal cytokine secretory activity in commonly used human breast cancer cell lines (MDA-MB-231, MCF-7, BT-474, and T-47D). Using MILLIPLEX assay, we measured the expression of 41 cytokines, including interleukins, monokines, interferons and growth factors. We also compared cytokine expression profile of breast cancer cells with those of non-tumorigenic human breast epithelial MCF-10A cells. Further, we investigated whether hypoxia modulates cytokine secretion of breast cancer cells. Using cobalt(II) chloride (CoCl(2)) to mimic hypoxia, we compared the effect of various treatment doses and intervals on cytokine production in the breast cancer cells. Results demonstrated that CoCl(2) affects the release of multiple cytokines in a dose- and concentration-dependent manner, thus highlighting the role of cancer cell-derived cytokines on breast tumor progression. Understanding the molecular actions of cytokines in the tumor microenvironment is important for understanding the mechanism of cancer initiation and progression. Oxford University Press 2020-05-08 /pmc/articles/PMC7207306/ http://dx.doi.org/10.1210/jendso/bvaa046.1328 Text en © Endocrine Society 2020. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Tumor Biology
Avtanski, Dimiter
Chen, Karin
Satlof, Leo
Stoffels, Guillaume
Kothapalli, Udithi
Ziluck, Noah
Lema, Maribel
Poretsky, Leonid
SAT-136 Hypoxia Effect on Cytokine Production by Breast Cancer Cells
title SAT-136 Hypoxia Effect on Cytokine Production by Breast Cancer Cells
title_full SAT-136 Hypoxia Effect on Cytokine Production by Breast Cancer Cells
title_fullStr SAT-136 Hypoxia Effect on Cytokine Production by Breast Cancer Cells
title_full_unstemmed SAT-136 Hypoxia Effect on Cytokine Production by Breast Cancer Cells
title_short SAT-136 Hypoxia Effect on Cytokine Production by Breast Cancer Cells
title_sort sat-136 hypoxia effect on cytokine production by breast cancer cells
topic Tumor Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7207306/
http://dx.doi.org/10.1210/jendso/bvaa046.1328
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