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OR24-07 Fetal Sex Impacts First Trimester Maternal-Fetal Communication in Humans
The placenta serves as a regulator of fetal growth throughout pregnancy. Signaling at the maternal-fetal interface is critical during placentation and lays the groundwork for placenta function, affecting pregnancy outcomes. Fetal growth is impacted by fetal sex, with males larger than females, and m...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7209053/ http://dx.doi.org/10.1210/jendso/bvaa046.1614 |
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author | Gonzalez, Tania L Sun, Tianyanxin Deng, Nan DiPentino, Rosemarie Clark, Ekaterina L Lee, Bora Wang, Yizhou Stripp, Barry R Yao, Changfu Tseng, Hsian-Rong Karumanchi, S A Koeppel, Alexander F Turner, Stephen D Farber, Charles R Rich, Stephen S Wang, Erica T Williams, John Pisarska, Margareta Danuta |
author_facet | Gonzalez, Tania L Sun, Tianyanxin Deng, Nan DiPentino, Rosemarie Clark, Ekaterina L Lee, Bora Wang, Yizhou Stripp, Barry R Yao, Changfu Tseng, Hsian-Rong Karumanchi, S A Koeppel, Alexander F Turner, Stephen D Farber, Charles R Rich, Stephen S Wang, Erica T Williams, John Pisarska, Margareta Danuta |
author_sort | Gonzalez, Tania L |
collection | PubMed |
description | The placenta serves as a regulator of fetal growth throughout pregnancy. Signaling at the maternal-fetal interface is critical during placentation and lays the groundwork for placenta function, affecting pregnancy outcomes. Fetal growth is impacted by fetal sex, with males larger than females, and maternal gestational diabetes and obesity independently increase the risk of macrosomia in male fetuses only. We previously demonstrated differentially expressed genes (DEGs) among sexes involves ancient canonical pathways and metabolic functions in placenta tissue. As these are likely impacted by signaling at the maternal-fetal interface, our aim here was to identify sex differences in signaling at the maternal-fetal interface and among individual cell types within the placenta to explain these differences. RNA-sequencing of first trimester placenta and maternal decidua as well as single cell RNA-sequencing in first trimester placenta was performed in ongoing pregnancies. We identified 91 sexually dimorphic receptor-ligand pairs across the maternal-fetal interface. From these, 35 of 115 receptors and/or ligand genes were also found to be upstream regulators of pathways critical in sexually dimorphic placentation which may define regulation. Single cell analysis identified five major cell types (trophoblasts, stromal cells, hofbauer cells, antigen presenting cells, and endothelial cells), and all had sexually dimorphic genes. Among individual cell types, ligands from the CC-family of cytokines were most highly representative in females, with their corresponding receptors present on the maternal surface. Furthermore, upstream regulator analysis of sexually dimorphic genes demonstrated TGFβ1 and estradiol to significantly affect all cell types. Dihydrotestosterone, which is produced by the male fetus, was an upstream regulator that was most significant for the trophoblast population. In addition, gene ontology enrichment analysis identified distinctive enriched functions between male and female trophoblasts, with cytokine mediated signaling pathways most representative. MUC15 and NOTUM were the most highly expressed sexually dimorphic autosomal genes found in distinct cell types of the trophoblast population, cell types critical for placentation and nutrient exchange. Thus, differences in hormone and immune signaling pathways may account for differential gene expression and differences in trophoblast function during placentation, which may in turn explain developmental differences, including fetal size, well-being, and overall outcomes. |
format | Online Article Text |
id | pubmed-7209053 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-72090532020-05-13 OR24-07 Fetal Sex Impacts First Trimester Maternal-Fetal Communication in Humans Gonzalez, Tania L Sun, Tianyanxin Deng, Nan DiPentino, Rosemarie Clark, Ekaterina L Lee, Bora Wang, Yizhou Stripp, Barry R Yao, Changfu Tseng, Hsian-Rong Karumanchi, S A Koeppel, Alexander F Turner, Stephen D Farber, Charles R Rich, Stephen S Wang, Erica T Williams, John Pisarska, Margareta Danuta J Endocr Soc Genetics and Development (including Gene Regulation) The placenta serves as a regulator of fetal growth throughout pregnancy. Signaling at the maternal-fetal interface is critical during placentation and lays the groundwork for placenta function, affecting pregnancy outcomes. Fetal growth is impacted by fetal sex, with males larger than females, and maternal gestational diabetes and obesity independently increase the risk of macrosomia in male fetuses only. We previously demonstrated differentially expressed genes (DEGs) among sexes involves ancient canonical pathways and metabolic functions in placenta tissue. As these are likely impacted by signaling at the maternal-fetal interface, our aim here was to identify sex differences in signaling at the maternal-fetal interface and among individual cell types within the placenta to explain these differences. RNA-sequencing of first trimester placenta and maternal decidua as well as single cell RNA-sequencing in first trimester placenta was performed in ongoing pregnancies. We identified 91 sexually dimorphic receptor-ligand pairs across the maternal-fetal interface. From these, 35 of 115 receptors and/or ligand genes were also found to be upstream regulators of pathways critical in sexually dimorphic placentation which may define regulation. Single cell analysis identified five major cell types (trophoblasts, stromal cells, hofbauer cells, antigen presenting cells, and endothelial cells), and all had sexually dimorphic genes. Among individual cell types, ligands from the CC-family of cytokines were most highly representative in females, with their corresponding receptors present on the maternal surface. Furthermore, upstream regulator analysis of sexually dimorphic genes demonstrated TGFβ1 and estradiol to significantly affect all cell types. Dihydrotestosterone, which is produced by the male fetus, was an upstream regulator that was most significant for the trophoblast population. In addition, gene ontology enrichment analysis identified distinctive enriched functions between male and female trophoblasts, with cytokine mediated signaling pathways most representative. MUC15 and NOTUM were the most highly expressed sexually dimorphic autosomal genes found in distinct cell types of the trophoblast population, cell types critical for placentation and nutrient exchange. Thus, differences in hormone and immune signaling pathways may account for differential gene expression and differences in trophoblast function during placentation, which may in turn explain developmental differences, including fetal size, well-being, and overall outcomes. Oxford University Press 2020-05-08 /pmc/articles/PMC7209053/ http://dx.doi.org/10.1210/jendso/bvaa046.1614 Text en © Endocrine Society 2020. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Genetics and Development (including Gene Regulation) Gonzalez, Tania L Sun, Tianyanxin Deng, Nan DiPentino, Rosemarie Clark, Ekaterina L Lee, Bora Wang, Yizhou Stripp, Barry R Yao, Changfu Tseng, Hsian-Rong Karumanchi, S A Koeppel, Alexander F Turner, Stephen D Farber, Charles R Rich, Stephen S Wang, Erica T Williams, John Pisarska, Margareta Danuta OR24-07 Fetal Sex Impacts First Trimester Maternal-Fetal Communication in Humans |
title | OR24-07 Fetal Sex Impacts First Trimester Maternal-Fetal Communication in Humans |
title_full | OR24-07 Fetal Sex Impacts First Trimester Maternal-Fetal Communication in Humans |
title_fullStr | OR24-07 Fetal Sex Impacts First Trimester Maternal-Fetal Communication in Humans |
title_full_unstemmed | OR24-07 Fetal Sex Impacts First Trimester Maternal-Fetal Communication in Humans |
title_short | OR24-07 Fetal Sex Impacts First Trimester Maternal-Fetal Communication in Humans |
title_sort | or24-07 fetal sex impacts first trimester maternal-fetal communication in humans |
topic | Genetics and Development (including Gene Regulation) |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7209053/ http://dx.doi.org/10.1210/jendso/bvaa046.1614 |
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