Cargando…

SUN-720 LC-MS/MS Measurements of Parathyroid Hormone-Related Protein (PTHrP): Association Between Ca, PTH and PTHrP in Clinical Samples

Parathyroid hormone related protein (PTHrP) is involved in intracellular calcium (Ca) regulation and homeostasis. The main clinical utility of PTHrP as a biomarker is to assist with determining the cause of unexplained hypercalcemia (UH). Considering that PTHrP, along with parathyroid hormone (PTH),...

Descripción completa

Detalles Bibliográficos
Autores principales: Kushnir, Mark M, Straseski, Joely A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7209161/
http://dx.doi.org/10.1210/jendso/bvaa046.533
_version_ 1783531010331246592
author Kushnir, Mark M
Straseski, Joely A
author_facet Kushnir, Mark M
Straseski, Joely A
author_sort Kushnir, Mark M
collection PubMed
description Parathyroid hormone related protein (PTHrP) is involved in intracellular calcium (Ca) regulation and homeostasis. The main clinical utility of PTHrP as a biomarker is to assist with determining the cause of unexplained hypercalcemia (UH). Considering that PTHrP, along with parathyroid hormone (PTH), is involved in Ca homeostasis, we evaluated associations between concentrations of Ca, PTH, PTHrP and age, and the between-sex differences in clinical patient samples. We reviewed historic data on Ca, PTH and PTHrP concentrations measured in routine patient samples (n=2,701) submitted for testing to a large clinical diagnostic laboratory. Among the analysed samples 31.1% were from adults (18-59y), 52.9% from older adults (60-79y) and 16.9% from elderly (>79y). Out of the samples, 60.5% were from women (W) and 39.5% from men (M). PTHrP was measured using a validated LC-MS/MS method; Ca and parathyroid hormone (PTH) were measured using the Cobas 8000 (Roche). The imprecision of all assays was <10%. Reference intervals (RI) for PTHrP were <3.3 pmol/L and <2.2 pmol/L in W and M, respectively; RI for calcium was 8.4–10.2 mg/dL, and for PTH was 15–65 pg/mL. Median (mean) PTHrP concentrations in samples from adults, older adults and elderly were 1.5 (7.9), 2.5 (8.7) and 3.2 (5.3) pmol/L, respectively. Median (mean) concentrations in samples from W and M were 2.4 (6.3) and 2.5 (10.4) pmol/L, respectively. Statistically significant difference in PTHrP concentrations was observed between W and M from adult (p=0.0005) and older adult (p=0.0038) groups; between W from adult and elderly groups (p=0.0113); and between M from older adult and elderly groups (p=0.028). No significant difference among the age groups was observed in concentrations of Ca and PTH. As expected, direct relationship was observed between concentrations of Ca and PTHrP (ρ=0.189, p<0.0001), while inverse relationships were observed between concentrations of Ca and PTH (ρ=-0.393, p<0.0001), and PTH and PTHrP (ρ=-0.226, p<0.0001). Assessment of the distribution of PTHrP concentrations in samples from W (n=472) and M (n=446) with UH (Ca >10.2 mg/dL and PTH< 15 pg/mL), revealed elevated PTHrP concentrations in 35.0% of W and 59.4% of M; with odds ratio for PTHrP as cause of UH of 0.7 and 2.2, in W and M, respectively. Among the study samples, PTHrP concentrations in 29.3% of samples from W and 54.2% of samples form M were above the sex specific RI. The PTHrP positivity rates in adult, older adult and elderly W (M) were 22.1%, 28.2% and 44.2% (41.2%, 58.5% and 70.5%), respectively. We observed higher prevalence of elevated PTHrP concentrations in M than in W, and in older individuals. PTHrP concentrations above the RI were observed in 39.1% of the analyzed samples. Our data suggest that hypercalcemia caused by elevated PTHrP concentrations is more common for pathologic conditions, which have higher prevalence or specific of M.
format Online
Article
Text
id pubmed-7209161
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-72091612020-05-13 SUN-720 LC-MS/MS Measurements of Parathyroid Hormone-Related Protein (PTHrP): Association Between Ca, PTH and PTHrP in Clinical Samples Kushnir, Mark M Straseski, Joely A J Endocr Soc Genetics and Development (including Gene Regulation) Parathyroid hormone related protein (PTHrP) is involved in intracellular calcium (Ca) regulation and homeostasis. The main clinical utility of PTHrP as a biomarker is to assist with determining the cause of unexplained hypercalcemia (UH). Considering that PTHrP, along with parathyroid hormone (PTH), is involved in Ca homeostasis, we evaluated associations between concentrations of Ca, PTH, PTHrP and age, and the between-sex differences in clinical patient samples. We reviewed historic data on Ca, PTH and PTHrP concentrations measured in routine patient samples (n=2,701) submitted for testing to a large clinical diagnostic laboratory. Among the analysed samples 31.1% were from adults (18-59y), 52.9% from older adults (60-79y) and 16.9% from elderly (>79y). Out of the samples, 60.5% were from women (W) and 39.5% from men (M). PTHrP was measured using a validated LC-MS/MS method; Ca and parathyroid hormone (PTH) were measured using the Cobas 8000 (Roche). The imprecision of all assays was <10%. Reference intervals (RI) for PTHrP were <3.3 pmol/L and <2.2 pmol/L in W and M, respectively; RI for calcium was 8.4–10.2 mg/dL, and for PTH was 15–65 pg/mL. Median (mean) PTHrP concentrations in samples from adults, older adults and elderly were 1.5 (7.9), 2.5 (8.7) and 3.2 (5.3) pmol/L, respectively. Median (mean) concentrations in samples from W and M were 2.4 (6.3) and 2.5 (10.4) pmol/L, respectively. Statistically significant difference in PTHrP concentrations was observed between W and M from adult (p=0.0005) and older adult (p=0.0038) groups; between W from adult and elderly groups (p=0.0113); and between M from older adult and elderly groups (p=0.028). No significant difference among the age groups was observed in concentrations of Ca and PTH. As expected, direct relationship was observed between concentrations of Ca and PTHrP (ρ=0.189, p<0.0001), while inverse relationships were observed between concentrations of Ca and PTH (ρ=-0.393, p<0.0001), and PTH and PTHrP (ρ=-0.226, p<0.0001). Assessment of the distribution of PTHrP concentrations in samples from W (n=472) and M (n=446) with UH (Ca >10.2 mg/dL and PTH< 15 pg/mL), revealed elevated PTHrP concentrations in 35.0% of W and 59.4% of M; with odds ratio for PTHrP as cause of UH of 0.7 and 2.2, in W and M, respectively. Among the study samples, PTHrP concentrations in 29.3% of samples from W and 54.2% of samples form M were above the sex specific RI. The PTHrP positivity rates in adult, older adult and elderly W (M) were 22.1%, 28.2% and 44.2% (41.2%, 58.5% and 70.5%), respectively. We observed higher prevalence of elevated PTHrP concentrations in M than in W, and in older individuals. PTHrP concentrations above the RI were observed in 39.1% of the analyzed samples. Our data suggest that hypercalcemia caused by elevated PTHrP concentrations is more common for pathologic conditions, which have higher prevalence or specific of M. Oxford University Press 2020-05-08 /pmc/articles/PMC7209161/ http://dx.doi.org/10.1210/jendso/bvaa046.533 Text en © Endocrine Society 2020. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Genetics and Development (including Gene Regulation)
Kushnir, Mark M
Straseski, Joely A
SUN-720 LC-MS/MS Measurements of Parathyroid Hormone-Related Protein (PTHrP): Association Between Ca, PTH and PTHrP in Clinical Samples
title SUN-720 LC-MS/MS Measurements of Parathyroid Hormone-Related Protein (PTHrP): Association Between Ca, PTH and PTHrP in Clinical Samples
title_full SUN-720 LC-MS/MS Measurements of Parathyroid Hormone-Related Protein (PTHrP): Association Between Ca, PTH and PTHrP in Clinical Samples
title_fullStr SUN-720 LC-MS/MS Measurements of Parathyroid Hormone-Related Protein (PTHrP): Association Between Ca, PTH and PTHrP in Clinical Samples
title_full_unstemmed SUN-720 LC-MS/MS Measurements of Parathyroid Hormone-Related Protein (PTHrP): Association Between Ca, PTH and PTHrP in Clinical Samples
title_short SUN-720 LC-MS/MS Measurements of Parathyroid Hormone-Related Protein (PTHrP): Association Between Ca, PTH and PTHrP in Clinical Samples
title_sort sun-720 lc-ms/ms measurements of parathyroid hormone-related protein (pthrp): association between ca, pth and pthrp in clinical samples
topic Genetics and Development (including Gene Regulation)
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7209161/
http://dx.doi.org/10.1210/jendso/bvaa046.533
work_keys_str_mv AT kushnirmarkm sun720lcmsmsmeasurementsofparathyroidhormonerelatedproteinpthrpassociationbetweencapthandpthrpinclinicalsamples
AT straseskijoelya sun720lcmsmsmeasurementsofparathyroidhormonerelatedproteinpthrpassociationbetweencapthandpthrpinclinicalsamples