Detection of drug resistant Mycobacterium tuberculosis by high-throughput sequencing of DNA isolated from acid fast bacilli smears

BACKGROUND: Drug susceptibility testing for Mycobacterium tuberculosis (MTB) is difficult to perform in resource-limited settings where Acid Fast Bacilli (AFB) smears are commonly used for disease diagnosis and monitoring. We developed a simple method for extraction of MTB DNA from AFB smears for se...

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Detalles Bibliográficos
Autores principales: Rowneki, Mazhgan, Aronson, Naomi, Du, Peicheng, Sachs, Paige, Blakemore, Robert, Chakravorty, Soumitesh, Levy, Shawn, Jones, Angela L., Trivedi, Geetika, Chebore, Sheilla, Addo, Dennis, Byarugaba, Denis K., Njobvu, Panganani Dalisani, Wabwire-Mangen, Frederick, Erima, Bernard, Ramos, Eric S., Evans, Carlton A, Hale, Braden, Mancuso, James D., Alland, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7209238/
https://www.ncbi.nlm.nih.gov/pubmed/32384098
http://dx.doi.org/10.1371/journal.pone.0232343

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