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SUN-585 Associations Between UCP1(rs1800592),UCP2(rs6593366) and UCP3(rs1800849) with Fasting Glucose, Body Mass Index, and Energy Expenditure

Background: The uncoupling proteins (UCPs) belong to the mitochondrial inner membrane anion carrier superfamily and play an important role in energy homeostasis. UCP-1 is expressed mostly in brown adipose tissue (BAT) and act in the thermogenesis and regulation of energy expenditure. UCP-2 has a rol...

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Autores principales: Figueroa-Vega, Nicté, Perez-Luque, Elva L, Malacara, Juan M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7209614/
http://dx.doi.org/10.1210/jendso/bvaa046.1973
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author Figueroa-Vega, Nicté
Perez-Luque, Elva L
Malacara, Juan M
author_facet Figueroa-Vega, Nicté
Perez-Luque, Elva L
Malacara, Juan M
author_sort Figueroa-Vega, Nicté
collection PubMed
description Background: The uncoupling proteins (UCPs) belong to the mitochondrial inner membrane anion carrier superfamily and play an important role in energy homeostasis. UCP-1 is expressed mostly in brown adipose tissue (BAT) and act in the thermogenesis and regulation of energy expenditure. UCP-2 has a role in the metabolism of fatty acids direct and indirectly path insulin secretion. UCP-3 is specific of skeletal muscle and BAT and may affects the adaptive and translocation of fatty acids. Genetic polymorphisms in these proteins have been associated with obesity, as rs1800592 (-3826 A/G) in UCP-1 gene. The rs659366 (-866GA) UCP-2 has being associated with high expression of its RNAm, decrease of obesity risk, and increase of energy expenditure. The rs1800849 (-55CT) UCP-3 has been associated with low risk of type 2 Diabetes Mellitus, but its association with body mass index is controversial. Objective: To analyze the association of the polymorphisms rs1800592 UCP-1, rs659366 UCP-2, and rs1800849 UCP-3 with BMI and resting energy expenditure (REE). Material and Methods: We included 120 subjects with BMI>30kg/m(2) and 100 subjects with BMI between18.5 -24.9 kg/m(2), aged 20 to 50 years. Anthropometric data were recorded and the REE was measure for indirect calorimetric. Fasting glucose and lipid profile were assessed. Leptin, insulin and acylated-ghrelin were quantified by ELISA. Genomic DNA was extracted using comercial kit. Genotyping for three polymorphisms was performed by allelic discrimination using Taqman probes. Results: All the three polymorphisms of UCPs showed distribution in accordance with Hardy-Weinberg equilibrium. The weight, BMI, glucose, triglycerides, leptin, insulin, HOMA-IR, and REE levels were signitifcantly higher in obese subjects. There was a strong correlation between REE with BMI (r=0.42, p<0.00001) and with insulin levels (r=0.229, p=0.001) in all group. No differences in genotypic and allelic frequencies of rs1800592 UCP-1, rs659366 UCP-2 and rs180084 UCP-3 polymorphisms between obese and lean subjects. No differences among the genotypes rs1800592 UCP-1 and rs1800849 UCP-3 with metabolic variables. In rs659366 UCP-2 polymorhism, the REE and glucose concentrations were lower in carriers of rs659366AA genotype (F=3.11, p=0.046; F=2.97, p=0.053, respectively) in whole group. In obese subjects with rs659366AA UCP-2 genotype, the REE was significantly low (F=4.15, P=0.017). Conclusion. In this work the obese subjects with rs659366AA genotype had low REE. We found low glucose concentrations in the carries of rs659366 AA genotype.
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spelling pubmed-72096142020-05-13 SUN-585 Associations Between UCP1(rs1800592),UCP2(rs6593366) and UCP3(rs1800849) with Fasting Glucose, Body Mass Index, and Energy Expenditure Figueroa-Vega, Nicté Perez-Luque, Elva L Malacara, Juan M J Endocr Soc Adipose Tissue, Appetite, and Obesity Background: The uncoupling proteins (UCPs) belong to the mitochondrial inner membrane anion carrier superfamily and play an important role in energy homeostasis. UCP-1 is expressed mostly in brown adipose tissue (BAT) and act in the thermogenesis and regulation of energy expenditure. UCP-2 has a role in the metabolism of fatty acids direct and indirectly path insulin secretion. UCP-3 is specific of skeletal muscle and BAT and may affects the adaptive and translocation of fatty acids. Genetic polymorphisms in these proteins have been associated with obesity, as rs1800592 (-3826 A/G) in UCP-1 gene. The rs659366 (-866GA) UCP-2 has being associated with high expression of its RNAm, decrease of obesity risk, and increase of energy expenditure. The rs1800849 (-55CT) UCP-3 has been associated with low risk of type 2 Diabetes Mellitus, but its association with body mass index is controversial. Objective: To analyze the association of the polymorphisms rs1800592 UCP-1, rs659366 UCP-2, and rs1800849 UCP-3 with BMI and resting energy expenditure (REE). Material and Methods: We included 120 subjects with BMI>30kg/m(2) and 100 subjects with BMI between18.5 -24.9 kg/m(2), aged 20 to 50 years. Anthropometric data were recorded and the REE was measure for indirect calorimetric. Fasting glucose and lipid profile were assessed. Leptin, insulin and acylated-ghrelin were quantified by ELISA. Genomic DNA was extracted using comercial kit. Genotyping for three polymorphisms was performed by allelic discrimination using Taqman probes. Results: All the three polymorphisms of UCPs showed distribution in accordance with Hardy-Weinberg equilibrium. The weight, BMI, glucose, triglycerides, leptin, insulin, HOMA-IR, and REE levels were signitifcantly higher in obese subjects. There was a strong correlation between REE with BMI (r=0.42, p<0.00001) and with insulin levels (r=0.229, p=0.001) in all group. No differences in genotypic and allelic frequencies of rs1800592 UCP-1, rs659366 UCP-2 and rs180084 UCP-3 polymorphisms between obese and lean subjects. No differences among the genotypes rs1800592 UCP-1 and rs1800849 UCP-3 with metabolic variables. In rs659366 UCP-2 polymorhism, the REE and glucose concentrations were lower in carriers of rs659366AA genotype (F=3.11, p=0.046; F=2.97, p=0.053, respectively) in whole group. In obese subjects with rs659366AA UCP-2 genotype, the REE was significantly low (F=4.15, P=0.017). Conclusion. In this work the obese subjects with rs659366AA genotype had low REE. We found low glucose concentrations in the carries of rs659366 AA genotype. Oxford University Press 2020-05-08 /pmc/articles/PMC7209614/ http://dx.doi.org/10.1210/jendso/bvaa046.1973 Text en © Endocrine Society 2020. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Adipose Tissue, Appetite, and Obesity
Figueroa-Vega, Nicté
Perez-Luque, Elva L
Malacara, Juan M
SUN-585 Associations Between UCP1(rs1800592),UCP2(rs6593366) and UCP3(rs1800849) with Fasting Glucose, Body Mass Index, and Energy Expenditure
title SUN-585 Associations Between UCP1(rs1800592),UCP2(rs6593366) and UCP3(rs1800849) with Fasting Glucose, Body Mass Index, and Energy Expenditure
title_full SUN-585 Associations Between UCP1(rs1800592),UCP2(rs6593366) and UCP3(rs1800849) with Fasting Glucose, Body Mass Index, and Energy Expenditure
title_fullStr SUN-585 Associations Between UCP1(rs1800592),UCP2(rs6593366) and UCP3(rs1800849) with Fasting Glucose, Body Mass Index, and Energy Expenditure
title_full_unstemmed SUN-585 Associations Between UCP1(rs1800592),UCP2(rs6593366) and UCP3(rs1800849) with Fasting Glucose, Body Mass Index, and Energy Expenditure
title_short SUN-585 Associations Between UCP1(rs1800592),UCP2(rs6593366) and UCP3(rs1800849) with Fasting Glucose, Body Mass Index, and Energy Expenditure
title_sort sun-585 associations between ucp1(rs1800592),ucp2(rs6593366) and ucp3(rs1800849) with fasting glucose, body mass index, and energy expenditure
topic Adipose Tissue, Appetite, and Obesity
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7209614/
http://dx.doi.org/10.1210/jendso/bvaa046.1973
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