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WFhb1-1 plays an important role in resistance against Fusarium head blight in wheat
Fusarium head blight (FHB) is a severe disease of wheat (Triticum aestivum L.). Qfhb1 is the most important quantitative trait locus (QTL) for FHB resistance. We previously identified wheat gene WFhb1-1 (aka WFhb1-c1) as a candidate for FHB resistance gene. Here we report that WFhb1-1 has been clone...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7210279/ https://www.ncbi.nlm.nih.gov/pubmed/32385328 http://dx.doi.org/10.1038/s41598-020-64777-9 |
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author | Paudel, Bimal Zhuang, Yongbin Galla, Aravind Dahal, Subha Qiu, Yinjie Ma, Anjun Raihan, Tajbir Yen, Yang |
author_facet | Paudel, Bimal Zhuang, Yongbin Galla, Aravind Dahal, Subha Qiu, Yinjie Ma, Anjun Raihan, Tajbir Yen, Yang |
author_sort | Paudel, Bimal |
collection | PubMed |
description | Fusarium head blight (FHB) is a severe disease of wheat (Triticum aestivum L.). Qfhb1 is the most important quantitative trait locus (QTL) for FHB resistance. We previously identified wheat gene WFhb1-1 (aka WFhb1-c1) as a candidate for FHB resistance gene. Here we report that WFhb1-1 has been cloned. The gene (GenBank # KU304333.1) consists of a single exon, encoding a putative membrane protein of 127 amino acids. WFhb1-1 protein produced in Pichia pastoris inhibits growth of both F. graminearum and P. pastoris in culture. Western Blotting with anti- WFhb1-1 antibody revealed a significant decrease (p < 0.01) in WFhb1-1 accumulation, 12 hours post Fusarium inoculation in non-Qfhb1-carrier wheat but not in Qfhb1-carrier wheat. Overexpressing WFhb1-1 in non-Qfhb1-carrier wheat led to a significant decrease (p < 0.01) in Fusarium-damaged rachis rate, Fusarium-diseased kernel rate and DON content in harvested kernels, while silencing WFhb1-1 in Qfhb1-carrier wheat resulted in a significant increase (p < 0.01) in FHB severity. Therefore, WFhb1-1 is an important FHB resistance gene with a potential antifungal function and probably a key functional component of Qfhb1 in wheat. A model regarding how WFhb1-1 functions in FHB resistance/susceptibility is hypothesized and discussed. |
format | Online Article Text |
id | pubmed-7210279 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-72102792020-05-15 WFhb1-1 plays an important role in resistance against Fusarium head blight in wheat Paudel, Bimal Zhuang, Yongbin Galla, Aravind Dahal, Subha Qiu, Yinjie Ma, Anjun Raihan, Tajbir Yen, Yang Sci Rep Article Fusarium head blight (FHB) is a severe disease of wheat (Triticum aestivum L.). Qfhb1 is the most important quantitative trait locus (QTL) for FHB resistance. We previously identified wheat gene WFhb1-1 (aka WFhb1-c1) as a candidate for FHB resistance gene. Here we report that WFhb1-1 has been cloned. The gene (GenBank # KU304333.1) consists of a single exon, encoding a putative membrane protein of 127 amino acids. WFhb1-1 protein produced in Pichia pastoris inhibits growth of both F. graminearum and P. pastoris in culture. Western Blotting with anti- WFhb1-1 antibody revealed a significant decrease (p < 0.01) in WFhb1-1 accumulation, 12 hours post Fusarium inoculation in non-Qfhb1-carrier wheat but not in Qfhb1-carrier wheat. Overexpressing WFhb1-1 in non-Qfhb1-carrier wheat led to a significant decrease (p < 0.01) in Fusarium-damaged rachis rate, Fusarium-diseased kernel rate and DON content in harvested kernels, while silencing WFhb1-1 in Qfhb1-carrier wheat resulted in a significant increase (p < 0.01) in FHB severity. Therefore, WFhb1-1 is an important FHB resistance gene with a potential antifungal function and probably a key functional component of Qfhb1 in wheat. A model regarding how WFhb1-1 functions in FHB resistance/susceptibility is hypothesized and discussed. Nature Publishing Group UK 2020-05-08 /pmc/articles/PMC7210279/ /pubmed/32385328 http://dx.doi.org/10.1038/s41598-020-64777-9 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Paudel, Bimal Zhuang, Yongbin Galla, Aravind Dahal, Subha Qiu, Yinjie Ma, Anjun Raihan, Tajbir Yen, Yang WFhb1-1 plays an important role in resistance against Fusarium head blight in wheat |
title | WFhb1-1 plays an important role in resistance against Fusarium head blight in wheat |
title_full | WFhb1-1 plays an important role in resistance against Fusarium head blight in wheat |
title_fullStr | WFhb1-1 plays an important role in resistance against Fusarium head blight in wheat |
title_full_unstemmed | WFhb1-1 plays an important role in resistance against Fusarium head blight in wheat |
title_short | WFhb1-1 plays an important role in resistance against Fusarium head blight in wheat |
title_sort | wfhb1-1 plays an important role in resistance against fusarium head blight in wheat |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7210279/ https://www.ncbi.nlm.nih.gov/pubmed/32385328 http://dx.doi.org/10.1038/s41598-020-64777-9 |
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