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Dietary lipids accumulate in macrophages and stromal cells and change the microarchitecture of mesenteric lymph nodes

In obesity, increased dietary lipids are taken up and transported by the lymphatic systems into the circulatory system. Increased fat accumulation results in impairments in the lymph fluid and lymph node (LN) atrophy. LNs filter the lymph fluid for foreign antigens to induce and control immune respo...

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Autores principales: Streich, Katharina, Smoczek, Margarethe, Hegermann, Jan, Dittrich-Breiholz, Oliver, Bornemann, Melanie, Siebert, Anja, Bleich, Andre, Buettner, Manuela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7210474/
https://www.ncbi.nlm.nih.gov/pubmed/32405435
http://dx.doi.org/10.1016/j.jare.2020.04.020
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author Streich, Katharina
Smoczek, Margarethe
Hegermann, Jan
Dittrich-Breiholz, Oliver
Bornemann, Melanie
Siebert, Anja
Bleich, Andre
Buettner, Manuela
author_facet Streich, Katharina
Smoczek, Margarethe
Hegermann, Jan
Dittrich-Breiholz, Oliver
Bornemann, Melanie
Siebert, Anja
Bleich, Andre
Buettner, Manuela
author_sort Streich, Katharina
collection PubMed
description In obesity, increased dietary lipids are taken up and transported by the lymphatic systems into the circulatory system. Increased fat accumulation results in impairments in the lymph fluid and lymph node (LN) atrophy. LNs filter the lymph fluid for foreign antigens to induce and control immune responses, and the alteration of this function during obesity remains underexplored. Here, the changes within the microarchitecture of mesenteric LNs (mLNs) during high levels of lipid transport were investigated, and the role of stromal cells in mice fed a high-fat diet for 10 weeks was assessed. Microarray experiments revealed that gene probes involved in lipid metabolism are expressed by mLN stromal cells. Transmission electron microscopy enabled the identification of lipid droplets in lymphatic endothelial cells, different reticulum cells, and macrophages, and the lipid droplet sizes as well as their numbers and intercellular distances increased after 10 weeks of high-fat diet feeding. The results indicate that changes in the microarchitecture and increased accumulation of lipid droplets in stromal cells and macrophages influence the immunological function of mLNs.
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spelling pubmed-72104742020-05-13 Dietary lipids accumulate in macrophages and stromal cells and change the microarchitecture of mesenteric lymph nodes Streich, Katharina Smoczek, Margarethe Hegermann, Jan Dittrich-Breiholz, Oliver Bornemann, Melanie Siebert, Anja Bleich, Andre Buettner, Manuela J Adv Res Article In obesity, increased dietary lipids are taken up and transported by the lymphatic systems into the circulatory system. Increased fat accumulation results in impairments in the lymph fluid and lymph node (LN) atrophy. LNs filter the lymph fluid for foreign antigens to induce and control immune responses, and the alteration of this function during obesity remains underexplored. Here, the changes within the microarchitecture of mesenteric LNs (mLNs) during high levels of lipid transport were investigated, and the role of stromal cells in mice fed a high-fat diet for 10 weeks was assessed. Microarray experiments revealed that gene probes involved in lipid metabolism are expressed by mLN stromal cells. Transmission electron microscopy enabled the identification of lipid droplets in lymphatic endothelial cells, different reticulum cells, and macrophages, and the lipid droplet sizes as well as their numbers and intercellular distances increased after 10 weeks of high-fat diet feeding. The results indicate that changes in the microarchitecture and increased accumulation of lipid droplets in stromal cells and macrophages influence the immunological function of mLNs. Elsevier 2020-04-29 /pmc/articles/PMC7210474/ /pubmed/32405435 http://dx.doi.org/10.1016/j.jare.2020.04.020 Text en © 2020 THE AUTHORS. Published by Elsevier BV on behalf of Cairo University. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Streich, Katharina
Smoczek, Margarethe
Hegermann, Jan
Dittrich-Breiholz, Oliver
Bornemann, Melanie
Siebert, Anja
Bleich, Andre
Buettner, Manuela
Dietary lipids accumulate in macrophages and stromal cells and change the microarchitecture of mesenteric lymph nodes
title Dietary lipids accumulate in macrophages and stromal cells and change the microarchitecture of mesenteric lymph nodes
title_full Dietary lipids accumulate in macrophages and stromal cells and change the microarchitecture of mesenteric lymph nodes
title_fullStr Dietary lipids accumulate in macrophages and stromal cells and change the microarchitecture of mesenteric lymph nodes
title_full_unstemmed Dietary lipids accumulate in macrophages and stromal cells and change the microarchitecture of mesenteric lymph nodes
title_short Dietary lipids accumulate in macrophages and stromal cells and change the microarchitecture of mesenteric lymph nodes
title_sort dietary lipids accumulate in macrophages and stromal cells and change the microarchitecture of mesenteric lymph nodes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7210474/
https://www.ncbi.nlm.nih.gov/pubmed/32405435
http://dx.doi.org/10.1016/j.jare.2020.04.020
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