Cargando…

Antiaging Activity of Peptide Identified from Fermented Trapa Japonica Fruit Extract in Human Dermal Fibroblasts

We have previously shown that Trapa japonica fruit extract (TJE) as well as its fermented extract (FTJ) can be potentially used to treat alopecia. In the current study, a newly synthesized peptide (PEP) was detected in an active compound isolated from FTJ. Several biological assays were conducted to...

Descripción completa

Detalles Bibliográficos
Autores principales: Jang, Jin Dong, Kim, Minkyung, Nam, Gun-He, Kim, Young-Min, Kang, Sang Moon, Lee, Kee-Young, Park, Young-Joon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7210532/
https://www.ncbi.nlm.nih.gov/pubmed/32419813
http://dx.doi.org/10.1155/2020/5895029
_version_ 1783531292451667968
author Jang, Jin Dong
Kim, Minkyung
Nam, Gun-He
Kim, Young-Min
Kang, Sang Moon
Lee, Kee-Young
Park, Young-Joon
author_facet Jang, Jin Dong
Kim, Minkyung
Nam, Gun-He
Kim, Young-Min
Kang, Sang Moon
Lee, Kee-Young
Park, Young-Joon
author_sort Jang, Jin Dong
collection PubMed
description We have previously shown that Trapa japonica fruit extract (TJE) as well as its fermented extract (FTJ) can be potentially used to treat alopecia. In the current study, a newly synthesized peptide (PEP) was detected in an active compound isolated from FTJ. Several biological assays were conducted to verify the antiaging effects of TJE, FTJ, and PEP on the skin. We examined the effects of TJE, FTJ, and PEP on cell viability, collagen synthesis, and inhibition of mRNA expression of matrix metalloproteinases (MMPs), induced by tumor necrosis factor alpha (TNF-α), in human dermal fibroblasts (HDFs). In addition, a wound-healing assay of the human keratinocyte cell line (HaCaT) and a clinical study of antiaging activity were conducted. The findings confirmed that PEP exerted an effect on cell proliferation in a dose-dependent manner. Treatment with TJE, FTJ, and PEP increased collagen synthesis but inhibited TNF-α-induced mRNA expression of MMPs. Compared with TJE and FTJ, PEP promoted a significant level of wound recovery in HaCaT cells and also exhibited antiaging effect, as demonstrated by a clinical study. These results suggest that PEP shows potential as a skin antiaging cosmetic product.
format Online
Article
Text
id pubmed-7210532
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-72105322020-05-15 Antiaging Activity of Peptide Identified from Fermented Trapa Japonica Fruit Extract in Human Dermal Fibroblasts Jang, Jin Dong Kim, Minkyung Nam, Gun-He Kim, Young-Min Kang, Sang Moon Lee, Kee-Young Park, Young-Joon Evid Based Complement Alternat Med Research Article We have previously shown that Trapa japonica fruit extract (TJE) as well as its fermented extract (FTJ) can be potentially used to treat alopecia. In the current study, a newly synthesized peptide (PEP) was detected in an active compound isolated from FTJ. Several biological assays were conducted to verify the antiaging effects of TJE, FTJ, and PEP on the skin. We examined the effects of TJE, FTJ, and PEP on cell viability, collagen synthesis, and inhibition of mRNA expression of matrix metalloproteinases (MMPs), induced by tumor necrosis factor alpha (TNF-α), in human dermal fibroblasts (HDFs). In addition, a wound-healing assay of the human keratinocyte cell line (HaCaT) and a clinical study of antiaging activity were conducted. The findings confirmed that PEP exerted an effect on cell proliferation in a dose-dependent manner. Treatment with TJE, FTJ, and PEP increased collagen synthesis but inhibited TNF-α-induced mRNA expression of MMPs. Compared with TJE and FTJ, PEP promoted a significant level of wound recovery in HaCaT cells and also exhibited antiaging effect, as demonstrated by a clinical study. These results suggest that PEP shows potential as a skin antiaging cosmetic product. Hindawi 2020-04-29 /pmc/articles/PMC7210532/ /pubmed/32419813 http://dx.doi.org/10.1155/2020/5895029 Text en Copyright © 2020 Jin Dong Jang et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Jang, Jin Dong
Kim, Minkyung
Nam, Gun-He
Kim, Young-Min
Kang, Sang Moon
Lee, Kee-Young
Park, Young-Joon
Antiaging Activity of Peptide Identified from Fermented Trapa Japonica Fruit Extract in Human Dermal Fibroblasts
title Antiaging Activity of Peptide Identified from Fermented Trapa Japonica Fruit Extract in Human Dermal Fibroblasts
title_full Antiaging Activity of Peptide Identified from Fermented Trapa Japonica Fruit Extract in Human Dermal Fibroblasts
title_fullStr Antiaging Activity of Peptide Identified from Fermented Trapa Japonica Fruit Extract in Human Dermal Fibroblasts
title_full_unstemmed Antiaging Activity of Peptide Identified from Fermented Trapa Japonica Fruit Extract in Human Dermal Fibroblasts
title_short Antiaging Activity of Peptide Identified from Fermented Trapa Japonica Fruit Extract in Human Dermal Fibroblasts
title_sort antiaging activity of peptide identified from fermented trapa japonica fruit extract in human dermal fibroblasts
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7210532/
https://www.ncbi.nlm.nih.gov/pubmed/32419813
http://dx.doi.org/10.1155/2020/5895029
work_keys_str_mv AT jangjindong antiagingactivityofpeptideidentifiedfromfermentedtrapajaponicafruitextractinhumandermalfibroblasts
AT kimminkyung antiagingactivityofpeptideidentifiedfromfermentedtrapajaponicafruitextractinhumandermalfibroblasts
AT namgunhe antiagingactivityofpeptideidentifiedfromfermentedtrapajaponicafruitextractinhumandermalfibroblasts
AT kimyoungmin antiagingactivityofpeptideidentifiedfromfermentedtrapajaponicafruitextractinhumandermalfibroblasts
AT kangsangmoon antiagingactivityofpeptideidentifiedfromfermentedtrapajaponicafruitextractinhumandermalfibroblasts
AT leekeeyoung antiagingactivityofpeptideidentifiedfromfermentedtrapajaponicafruitextractinhumandermalfibroblasts
AT parkyoungjoon antiagingactivityofpeptideidentifiedfromfermentedtrapajaponicafruitextractinhumandermalfibroblasts