Cargando…

The calcineurin β-like interacting protein kinase CIPK25 regulates potassium homeostasis under low oxygen in Arabidopsis

Hypoxic conditions often arise from waterlogging and flooding, affecting several aspects of plant metabolism, including the uptake of nutrients. We identified a member of the CALCINEURIN β-LIKE INTERACTING PROTEIN KINASE (CIPK) family in Arabidopsis, CIPK25, which is induced in the root endodermis u...

Descripción completa

Detalles Bibliográficos
Autores principales: Tagliani, Andrea, Tran, Anh Nguyet, Novi, Giacomo, Di Mambro, Riccardo, Pesenti, Michele, Sacchi, Gian Attilio, Perata, Pierdomenico, Pucciariello, Chiara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7210770/
https://www.ncbi.nlm.nih.gov/pubmed/32053194
http://dx.doi.org/10.1093/jxb/eraa004
_version_ 1783531326383587328
author Tagliani, Andrea
Tran, Anh Nguyet
Novi, Giacomo
Di Mambro, Riccardo
Pesenti, Michele
Sacchi, Gian Attilio
Perata, Pierdomenico
Pucciariello, Chiara
author_facet Tagliani, Andrea
Tran, Anh Nguyet
Novi, Giacomo
Di Mambro, Riccardo
Pesenti, Michele
Sacchi, Gian Attilio
Perata, Pierdomenico
Pucciariello, Chiara
author_sort Tagliani, Andrea
collection PubMed
description Hypoxic conditions often arise from waterlogging and flooding, affecting several aspects of plant metabolism, including the uptake of nutrients. We identified a member of the CALCINEURIN β-LIKE INTERACTING PROTEIN KINASE (CIPK) family in Arabidopsis, CIPK25, which is induced in the root endodermis under low-oxygen conditions. A cipk25 mutant exhibited higher sensitivity to anoxia in conditions of potassium limitation, suggesting that this kinase is involved in the regulation of potassium uptake. Interestingly, we found that CIPK25 interacts with AKT1, the major inward rectifying potassium channel in Arabidopsis. Under anoxic conditions, cipk25 mutant seedlings were unable to maintain potassium concentrations at wild-type levels, suggesting that CIPK25 likely plays a role in modulating potassium homeostasis under low-oxygen conditions. In addition, cipk25 and akt1 mutants share similar developmental defects under waterlogging, further supporting an interplay between CIPK25 and AKT1.
format Online
Article
Text
id pubmed-7210770
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-72107702020-05-14 The calcineurin β-like interacting protein kinase CIPK25 regulates potassium homeostasis under low oxygen in Arabidopsis Tagliani, Andrea Tran, Anh Nguyet Novi, Giacomo Di Mambro, Riccardo Pesenti, Michele Sacchi, Gian Attilio Perata, Pierdomenico Pucciariello, Chiara J Exp Bot Research Papers Hypoxic conditions often arise from waterlogging and flooding, affecting several aspects of plant metabolism, including the uptake of nutrients. We identified a member of the CALCINEURIN β-LIKE INTERACTING PROTEIN KINASE (CIPK) family in Arabidopsis, CIPK25, which is induced in the root endodermis under low-oxygen conditions. A cipk25 mutant exhibited higher sensitivity to anoxia in conditions of potassium limitation, suggesting that this kinase is involved in the regulation of potassium uptake. Interestingly, we found that CIPK25 interacts with AKT1, the major inward rectifying potassium channel in Arabidopsis. Under anoxic conditions, cipk25 mutant seedlings were unable to maintain potassium concentrations at wild-type levels, suggesting that CIPK25 likely plays a role in modulating potassium homeostasis under low-oxygen conditions. In addition, cipk25 and akt1 mutants share similar developmental defects under waterlogging, further supporting an interplay between CIPK25 and AKT1. Oxford University Press 2020-05-09 2020-02-13 /pmc/articles/PMC7210770/ /pubmed/32053194 http://dx.doi.org/10.1093/jxb/eraa004 Text en © The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Papers
Tagliani, Andrea
Tran, Anh Nguyet
Novi, Giacomo
Di Mambro, Riccardo
Pesenti, Michele
Sacchi, Gian Attilio
Perata, Pierdomenico
Pucciariello, Chiara
The calcineurin β-like interacting protein kinase CIPK25 regulates potassium homeostasis under low oxygen in Arabidopsis
title The calcineurin β-like interacting protein kinase CIPK25 regulates potassium homeostasis under low oxygen in Arabidopsis
title_full The calcineurin β-like interacting protein kinase CIPK25 regulates potassium homeostasis under low oxygen in Arabidopsis
title_fullStr The calcineurin β-like interacting protein kinase CIPK25 regulates potassium homeostasis under low oxygen in Arabidopsis
title_full_unstemmed The calcineurin β-like interacting protein kinase CIPK25 regulates potassium homeostasis under low oxygen in Arabidopsis
title_short The calcineurin β-like interacting protein kinase CIPK25 regulates potassium homeostasis under low oxygen in Arabidopsis
title_sort calcineurin β-like interacting protein kinase cipk25 regulates potassium homeostasis under low oxygen in arabidopsis
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7210770/
https://www.ncbi.nlm.nih.gov/pubmed/32053194
http://dx.doi.org/10.1093/jxb/eraa004
work_keys_str_mv AT taglianiandrea thecalcineurinblikeinteractingproteinkinasecipk25regulatespotassiumhomeostasisunderlowoxygeninarabidopsis
AT trananhnguyet thecalcineurinblikeinteractingproteinkinasecipk25regulatespotassiumhomeostasisunderlowoxygeninarabidopsis
AT novigiacomo thecalcineurinblikeinteractingproteinkinasecipk25regulatespotassiumhomeostasisunderlowoxygeninarabidopsis
AT dimambroriccardo thecalcineurinblikeinteractingproteinkinasecipk25regulatespotassiumhomeostasisunderlowoxygeninarabidopsis
AT pesentimichele thecalcineurinblikeinteractingproteinkinasecipk25regulatespotassiumhomeostasisunderlowoxygeninarabidopsis
AT sacchigianattilio thecalcineurinblikeinteractingproteinkinasecipk25regulatespotassiumhomeostasisunderlowoxygeninarabidopsis
AT peratapierdomenico thecalcineurinblikeinteractingproteinkinasecipk25regulatespotassiumhomeostasisunderlowoxygeninarabidopsis
AT pucciariellochiara thecalcineurinblikeinteractingproteinkinasecipk25regulatespotassiumhomeostasisunderlowoxygeninarabidopsis
AT taglianiandrea calcineurinblikeinteractingproteinkinasecipk25regulatespotassiumhomeostasisunderlowoxygeninarabidopsis
AT trananhnguyet calcineurinblikeinteractingproteinkinasecipk25regulatespotassiumhomeostasisunderlowoxygeninarabidopsis
AT novigiacomo calcineurinblikeinteractingproteinkinasecipk25regulatespotassiumhomeostasisunderlowoxygeninarabidopsis
AT dimambroriccardo calcineurinblikeinteractingproteinkinasecipk25regulatespotassiumhomeostasisunderlowoxygeninarabidopsis
AT pesentimichele calcineurinblikeinteractingproteinkinasecipk25regulatespotassiumhomeostasisunderlowoxygeninarabidopsis
AT sacchigianattilio calcineurinblikeinteractingproteinkinasecipk25regulatespotassiumhomeostasisunderlowoxygeninarabidopsis
AT peratapierdomenico calcineurinblikeinteractingproteinkinasecipk25regulatespotassiumhomeostasisunderlowoxygeninarabidopsis
AT pucciariellochiara calcineurinblikeinteractingproteinkinasecipk25regulatespotassiumhomeostasisunderlowoxygeninarabidopsis