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Diversity of cultivable protease-producing bacteria and their extracellular proteases associated to scleractinian corals
Protease-producing bacteria play a vital role in degrading organic nitrogen in marine environments. However, the diversity of the bacteria and extracellular proteases has seldom been addressed, especially in communities of coral reefs. In this study, 136 extracellular protease-producing bacterial st...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7210813/ https://www.ncbi.nlm.nih.gov/pubmed/32411529 http://dx.doi.org/10.7717/peerj.9055 |
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author | Su, Hongfei Xiao, Zhenlun Yu, Kefu Huang, Qinyu Wang, Guanghua Wang, Yinghui Liang, Jiayuan Huang, Wen Huang, Xueyong Wei, Fen Chen, Biao |
author_facet | Su, Hongfei Xiao, Zhenlun Yu, Kefu Huang, Qinyu Wang, Guanghua Wang, Yinghui Liang, Jiayuan Huang, Wen Huang, Xueyong Wei, Fen Chen, Biao |
author_sort | Su, Hongfei |
collection | PubMed |
description | Protease-producing bacteria play a vital role in degrading organic nitrogen in marine environments. However, the diversity of the bacteria and extracellular proteases has seldom been addressed, especially in communities of coral reefs. In this study, 136 extracellular protease-producing bacterial strains were isolated from seven genera of scleractinian corals from Luhuitou fringing reef, and their protease types were characterized. The massive coral had more cultivable protease-producing bacteria than branching or foliose corals. The abundance of cultivable protease-producing bacteria reached 10(6) CFU g(−1) of coral. Phylogenetic analysis of 16S rRNA gene sequences revealed that the isolates were assigned to 24 genera, from which 20 corresponded to the phyla Firmicutes and Proteobacteria. Bacillus and Fictibacillus were retrieved from all coral samples. Moreover, Vibrio and Pseudovibrio were most prevalent in massive or foliose coral Platygyra and Montipora. In contrast, 11 genera were each identified in only one isolate. Nearly all the extracellular proteases from the bacteria were serine proteases or metalloproteases; 45.83% of isolates also released cysteine or aspartic proteases. These proteases had different hydrolytic ability against different substrates. This study represents a novel insight on the diversity of cultivable protease-producing bacteria and their extracellular proteases in scleractinian corals. |
format | Online Article Text |
id | pubmed-7210813 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-72108132020-05-14 Diversity of cultivable protease-producing bacteria and their extracellular proteases associated to scleractinian corals Su, Hongfei Xiao, Zhenlun Yu, Kefu Huang, Qinyu Wang, Guanghua Wang, Yinghui Liang, Jiayuan Huang, Wen Huang, Xueyong Wei, Fen Chen, Biao PeerJ Marine Biology Protease-producing bacteria play a vital role in degrading organic nitrogen in marine environments. However, the diversity of the bacteria and extracellular proteases has seldom been addressed, especially in communities of coral reefs. In this study, 136 extracellular protease-producing bacterial strains were isolated from seven genera of scleractinian corals from Luhuitou fringing reef, and their protease types were characterized. The massive coral had more cultivable protease-producing bacteria than branching or foliose corals. The abundance of cultivable protease-producing bacteria reached 10(6) CFU g(−1) of coral. Phylogenetic analysis of 16S rRNA gene sequences revealed that the isolates were assigned to 24 genera, from which 20 corresponded to the phyla Firmicutes and Proteobacteria. Bacillus and Fictibacillus were retrieved from all coral samples. Moreover, Vibrio and Pseudovibrio were most prevalent in massive or foliose coral Platygyra and Montipora. In contrast, 11 genera were each identified in only one isolate. Nearly all the extracellular proteases from the bacteria were serine proteases or metalloproteases; 45.83% of isolates also released cysteine or aspartic proteases. These proteases had different hydrolytic ability against different substrates. This study represents a novel insight on the diversity of cultivable protease-producing bacteria and their extracellular proteases in scleractinian corals. PeerJ Inc. 2020-05-06 /pmc/articles/PMC7210813/ /pubmed/32411529 http://dx.doi.org/10.7717/peerj.9055 Text en ©2020 Su et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Marine Biology Su, Hongfei Xiao, Zhenlun Yu, Kefu Huang, Qinyu Wang, Guanghua Wang, Yinghui Liang, Jiayuan Huang, Wen Huang, Xueyong Wei, Fen Chen, Biao Diversity of cultivable protease-producing bacteria and their extracellular proteases associated to scleractinian corals |
title | Diversity of cultivable protease-producing bacteria and their extracellular proteases associated to scleractinian corals |
title_full | Diversity of cultivable protease-producing bacteria and their extracellular proteases associated to scleractinian corals |
title_fullStr | Diversity of cultivable protease-producing bacteria and their extracellular proteases associated to scleractinian corals |
title_full_unstemmed | Diversity of cultivable protease-producing bacteria and their extracellular proteases associated to scleractinian corals |
title_short | Diversity of cultivable protease-producing bacteria and their extracellular proteases associated to scleractinian corals |
title_sort | diversity of cultivable protease-producing bacteria and their extracellular proteases associated to scleractinian corals |
topic | Marine Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7210813/ https://www.ncbi.nlm.nih.gov/pubmed/32411529 http://dx.doi.org/10.7717/peerj.9055 |
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