Spatially resolved and multiplexed MicroRNA quantification from tissue using nanoliter well arrays

Spatially resolved gene expression patterns are emerging as a key component of medical studies, including companion diagnostics, but technologies for quantification and multiplexing are limited. We present a method to perform spatially resolved and multiplexed microRNA (miRNA) measurements from form...

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Detalles Bibliográficos
Autores principales: Nagarajan, Maxwell B., Tentori, Augusto M., Zhang, Wen Cai, Slack, Frank J., Doyle, Patrick S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7211184/
https://www.ncbi.nlm.nih.gov/pubmed/32419951
http://dx.doi.org/10.1038/s41378-020-0169-8
Descripción
Sumario:Spatially resolved gene expression patterns are emerging as a key component of medical studies, including companion diagnostics, but technologies for quantification and multiplexing are limited. We present a method to perform spatially resolved and multiplexed microRNA (miRNA) measurements from formalin-fixed, paraffin-embedded (FFPE) tissue. Using nanoliter well arrays to pixelate the tissue section and photopatterned hydrogels to quantify miRNA, we identified differentially expressed miRNAs in tumors from a genetically engineered mouse model for non-small cell lung cancer (K-ras(LSL-G12D/+); p53(fl/fl)). This technology could be used to quantify heterogeneities in tissue samples and lead to informed, biomarker-based diagnostics.

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