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Spatially resolved and multiplexed MicroRNA quantification from tissue using nanoliter well arrays

Spatially resolved gene expression patterns are emerging as a key component of medical studies, including companion diagnostics, but technologies for quantification and multiplexing are limited. We present a method to perform spatially resolved and multiplexed microRNA (miRNA) measurements from form...

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Autores principales: Nagarajan, Maxwell B., Tentori, Augusto M., Zhang, Wen Cai, Slack, Frank J., Doyle, Patrick S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7211184/
https://www.ncbi.nlm.nih.gov/pubmed/32419951
http://dx.doi.org/10.1038/s41378-020-0169-8
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author Nagarajan, Maxwell B.
Tentori, Augusto M.
Zhang, Wen Cai
Slack, Frank J.
Doyle, Patrick S.
author_facet Nagarajan, Maxwell B.
Tentori, Augusto M.
Zhang, Wen Cai
Slack, Frank J.
Doyle, Patrick S.
author_sort Nagarajan, Maxwell B.
collection PubMed
description Spatially resolved gene expression patterns are emerging as a key component of medical studies, including companion diagnostics, but technologies for quantification and multiplexing are limited. We present a method to perform spatially resolved and multiplexed microRNA (miRNA) measurements from formalin-fixed, paraffin-embedded (FFPE) tissue. Using nanoliter well arrays to pixelate the tissue section and photopatterned hydrogels to quantify miRNA, we identified differentially expressed miRNAs in tumors from a genetically engineered mouse model for non-small cell lung cancer (K-ras(LSL-G12D/+); p53(fl/fl)). This technology could be used to quantify heterogeneities in tissue samples and lead to informed, biomarker-based diagnostics.
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spelling pubmed-72111842020-05-15 Spatially resolved and multiplexed MicroRNA quantification from tissue using nanoliter well arrays Nagarajan, Maxwell B. Tentori, Augusto M. Zhang, Wen Cai Slack, Frank J. Doyle, Patrick S. Microsyst Nanoeng Article Spatially resolved gene expression patterns are emerging as a key component of medical studies, including companion diagnostics, but technologies for quantification and multiplexing are limited. We present a method to perform spatially resolved and multiplexed microRNA (miRNA) measurements from formalin-fixed, paraffin-embedded (FFPE) tissue. Using nanoliter well arrays to pixelate the tissue section and photopatterned hydrogels to quantify miRNA, we identified differentially expressed miRNAs in tumors from a genetically engineered mouse model for non-small cell lung cancer (K-ras(LSL-G12D/+); p53(fl/fl)). This technology could be used to quantify heterogeneities in tissue samples and lead to informed, biomarker-based diagnostics. Nature Publishing Group UK 2020-05-09 /pmc/articles/PMC7211184/ /pubmed/32419951 http://dx.doi.org/10.1038/s41378-020-0169-8 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Nagarajan, Maxwell B.
Tentori, Augusto M.
Zhang, Wen Cai
Slack, Frank J.
Doyle, Patrick S.
Spatially resolved and multiplexed MicroRNA quantification from tissue using nanoliter well arrays
title Spatially resolved and multiplexed MicroRNA quantification from tissue using nanoliter well arrays
title_full Spatially resolved and multiplexed MicroRNA quantification from tissue using nanoliter well arrays
title_fullStr Spatially resolved and multiplexed MicroRNA quantification from tissue using nanoliter well arrays
title_full_unstemmed Spatially resolved and multiplexed MicroRNA quantification from tissue using nanoliter well arrays
title_short Spatially resolved and multiplexed MicroRNA quantification from tissue using nanoliter well arrays
title_sort spatially resolved and multiplexed microrna quantification from tissue using nanoliter well arrays
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7211184/
https://www.ncbi.nlm.nih.gov/pubmed/32419951
http://dx.doi.org/10.1038/s41378-020-0169-8
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