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Spatially resolved and multiplexed MicroRNA quantification from tissue using nanoliter well arrays
Spatially resolved gene expression patterns are emerging as a key component of medical studies, including companion diagnostics, but technologies for quantification and multiplexing are limited. We present a method to perform spatially resolved and multiplexed microRNA (miRNA) measurements from form...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7211184/ https://www.ncbi.nlm.nih.gov/pubmed/32419951 http://dx.doi.org/10.1038/s41378-020-0169-8 |
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author | Nagarajan, Maxwell B. Tentori, Augusto M. Zhang, Wen Cai Slack, Frank J. Doyle, Patrick S. |
author_facet | Nagarajan, Maxwell B. Tentori, Augusto M. Zhang, Wen Cai Slack, Frank J. Doyle, Patrick S. |
author_sort | Nagarajan, Maxwell B. |
collection | PubMed |
description | Spatially resolved gene expression patterns are emerging as a key component of medical studies, including companion diagnostics, but technologies for quantification and multiplexing are limited. We present a method to perform spatially resolved and multiplexed microRNA (miRNA) measurements from formalin-fixed, paraffin-embedded (FFPE) tissue. Using nanoliter well arrays to pixelate the tissue section and photopatterned hydrogels to quantify miRNA, we identified differentially expressed miRNAs in tumors from a genetically engineered mouse model for non-small cell lung cancer (K-ras(LSL-G12D/+); p53(fl/fl)). This technology could be used to quantify heterogeneities in tissue samples and lead to informed, biomarker-based diagnostics. |
format | Online Article Text |
id | pubmed-7211184 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-72111842020-05-15 Spatially resolved and multiplexed MicroRNA quantification from tissue using nanoliter well arrays Nagarajan, Maxwell B. Tentori, Augusto M. Zhang, Wen Cai Slack, Frank J. Doyle, Patrick S. Microsyst Nanoeng Article Spatially resolved gene expression patterns are emerging as a key component of medical studies, including companion diagnostics, but technologies for quantification and multiplexing are limited. We present a method to perform spatially resolved and multiplexed microRNA (miRNA) measurements from formalin-fixed, paraffin-embedded (FFPE) tissue. Using nanoliter well arrays to pixelate the tissue section and photopatterned hydrogels to quantify miRNA, we identified differentially expressed miRNAs in tumors from a genetically engineered mouse model for non-small cell lung cancer (K-ras(LSL-G12D/+); p53(fl/fl)). This technology could be used to quantify heterogeneities in tissue samples and lead to informed, biomarker-based diagnostics. Nature Publishing Group UK 2020-05-09 /pmc/articles/PMC7211184/ /pubmed/32419951 http://dx.doi.org/10.1038/s41378-020-0169-8 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Nagarajan, Maxwell B. Tentori, Augusto M. Zhang, Wen Cai Slack, Frank J. Doyle, Patrick S. Spatially resolved and multiplexed MicroRNA quantification from tissue using nanoliter well arrays |
title | Spatially resolved and multiplexed MicroRNA quantification from tissue using nanoliter well arrays |
title_full | Spatially resolved and multiplexed MicroRNA quantification from tissue using nanoliter well arrays |
title_fullStr | Spatially resolved and multiplexed MicroRNA quantification from tissue using nanoliter well arrays |
title_full_unstemmed | Spatially resolved and multiplexed MicroRNA quantification from tissue using nanoliter well arrays |
title_short | Spatially resolved and multiplexed MicroRNA quantification from tissue using nanoliter well arrays |
title_sort | spatially resolved and multiplexed microrna quantification from tissue using nanoliter well arrays |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7211184/ https://www.ncbi.nlm.nih.gov/pubmed/32419951 http://dx.doi.org/10.1038/s41378-020-0169-8 |
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