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Metastasis inhibition by BRMS1 and miR-31 replacement therapy in claudin-low cell lines

OBJECTIVE(S): The growing trend of research demonstrates that dynamic expression of two metastasis repressor classes (metastasis suppressor genes and anti-metastatic miRNA) has a close relationship with tumor invasion and metastasis. Using different strategies, it was revealed that cellular levels o...

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Autores principales: Farokhimanesh, Samila, Forouzandeh Moghadam, Mehdi, Ebrahimi, Marzieh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7211348/
https://www.ncbi.nlm.nih.gov/pubmed/32405371
http://dx.doi.org/10.22038/IJBMS.2019.35674.8500
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author Farokhimanesh, Samila
Forouzandeh Moghadam, Mehdi
Ebrahimi, Marzieh
author_facet Farokhimanesh, Samila
Forouzandeh Moghadam, Mehdi
Ebrahimi, Marzieh
author_sort Farokhimanesh, Samila
collection PubMed
description OBJECTIVE(S): The growing trend of research demonstrates that dynamic expression of two metastasis repressor classes (metastasis suppressor genes and anti-metastatic miRNA) has a close relationship with tumor invasion and metastasis. Using different strategies, it was revealed that cellular levels of miR-31 and Breast cancer Metastasis Suppressor1 (BRMS1) protein, which are among the most significant modulators of metastasis, have a correlation with the cell’s capability for invading and metastasizing; cells containing higher levels of miR-31 or BRMS1 were less metastatic. This project was carried out to determine whether the combinations of miR-31 and BRMS1 genes are able to enhance the capability of repressing the claudin-low breast cancer cell (MDA-MB-231) invasion. MATERIALS AND METHODS: This study used a restoration-based approach by miR-31 mimic and optimized BRMS1 gene sequences, which were cloned into a chimeric construct and transfected to the MDA-M231cells. RESULTS: Our data revealed that the simultaneous expression of anti-metastasis miR and metastasis suppressor might inhibit migration and invasion in MDA-MB-231 cells efficiently. CONCLUSION: This combinatorial use of anti-metastatic miR and gene suggests a new therapeutic intervention for metastasis inhibition in MDA-MB-231.
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spelling pubmed-72113482020-05-13 Metastasis inhibition by BRMS1 and miR-31 replacement therapy in claudin-low cell lines Farokhimanesh, Samila Forouzandeh Moghadam, Mehdi Ebrahimi, Marzieh Iran J Basic Med Sci Original Article OBJECTIVE(S): The growing trend of research demonstrates that dynamic expression of two metastasis repressor classes (metastasis suppressor genes and anti-metastatic miRNA) has a close relationship with tumor invasion and metastasis. Using different strategies, it was revealed that cellular levels of miR-31 and Breast cancer Metastasis Suppressor1 (BRMS1) protein, which are among the most significant modulators of metastasis, have a correlation with the cell’s capability for invading and metastasizing; cells containing higher levels of miR-31 or BRMS1 were less metastatic. This project was carried out to determine whether the combinations of miR-31 and BRMS1 genes are able to enhance the capability of repressing the claudin-low breast cancer cell (MDA-MB-231) invasion. MATERIALS AND METHODS: This study used a restoration-based approach by miR-31 mimic and optimized BRMS1 gene sequences, which were cloned into a chimeric construct and transfected to the MDA-M231cells. RESULTS: Our data revealed that the simultaneous expression of anti-metastasis miR and metastasis suppressor might inhibit migration and invasion in MDA-MB-231 cells efficiently. CONCLUSION: This combinatorial use of anti-metastatic miR and gene suggests a new therapeutic intervention for metastasis inhibition in MDA-MB-231. Mashhad University of Medical Sciences 2020-02 /pmc/articles/PMC7211348/ /pubmed/32405371 http://dx.doi.org/10.22038/IJBMS.2019.35674.8500 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Farokhimanesh, Samila
Forouzandeh Moghadam, Mehdi
Ebrahimi, Marzieh
Metastasis inhibition by BRMS1 and miR-31 replacement therapy in claudin-low cell lines
title Metastasis inhibition by BRMS1 and miR-31 replacement therapy in claudin-low cell lines
title_full Metastasis inhibition by BRMS1 and miR-31 replacement therapy in claudin-low cell lines
title_fullStr Metastasis inhibition by BRMS1 and miR-31 replacement therapy in claudin-low cell lines
title_full_unstemmed Metastasis inhibition by BRMS1 and miR-31 replacement therapy in claudin-low cell lines
title_short Metastasis inhibition by BRMS1 and miR-31 replacement therapy in claudin-low cell lines
title_sort metastasis inhibition by brms1 and mir-31 replacement therapy in claudin-low cell lines
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7211348/
https://www.ncbi.nlm.nih.gov/pubmed/32405371
http://dx.doi.org/10.22038/IJBMS.2019.35674.8500
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