Rapid, modular, and cost-effective generation of donor DNA constructs for CRISPR-based gene knock-in

Clustered regularly interspaced short palindromic repeats (CRISPR)-based gene editing techniques find applications in many fields, such as molecular biology, cancer biology, and disease modeling. In contrast to the knock-out procedure, a key step of CRISPR knock-in experiments is the homology-direct...

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Detalles Bibliográficos
Autores principales: Chen, Yi-Jiun, Cheng, Ya-Yun, Wang, Weikang, Tian, Xiao-Jun, Lefever, Daniel E, Taft, David A, Zhang, Jingyu, Xing, Jianhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Methods Manuscript
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7211398/
https://www.ncbi.nlm.nih.gov/pubmed/32411820
http://dx.doi.org/10.1093/biomethods/bpaa006

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