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Controlled release of basic fibroblast growth factor from a peptide biomaterial for bone regeneration

Self-assembled peptide scaffolds based on D-RADA16 are an important matrix for controlled drug release and three-dimensional cell culture. In this work, D-RADA16 peptide hydrogels were coated on artificial bone composed of nano-hydroxyapatite/polyamide 66 (nHA/PA66) to obtain a porous drug-releasing...

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Autores principales: Zhao, WeiKang, Li, Yuling, Zhou, Ao, Chen, Xiaojun, Li, Kai, Chen, Sinan, Qiao, Bo, Jiang, Dianming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7211882/
https://www.ncbi.nlm.nih.gov/pubmed/32431879
http://dx.doi.org/10.1098/rsos.191830
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author Zhao, WeiKang
Li, Yuling
Zhou, Ao
Chen, Xiaojun
Li, Kai
Chen, Sinan
Qiao, Bo
Jiang, Dianming
author_facet Zhao, WeiKang
Li, Yuling
Zhou, Ao
Chen, Xiaojun
Li, Kai
Chen, Sinan
Qiao, Bo
Jiang, Dianming
author_sort Zhao, WeiKang
collection PubMed
description Self-assembled peptide scaffolds based on D-RADA16 are an important matrix for controlled drug release and three-dimensional cell culture. In this work, D-RADA16 peptide hydrogels were coated on artificial bone composed of nano-hydroxyapatite/polyamide 66 (nHA/PA66) to obtain a porous drug-releasing structure for treating bone defects. The developed materials were characterized via transmission electron microscopy and scanning electron microscopy. The proliferation and adhesion of bone mesenchymal stem cells (BMSCs) were examined by confocal laser microscopy and CCK-8 experiments. The osteogenic ability of the porous materials towards bone BMSCs was examined in vitro by staining with Alizarin Red S and alkaline phosphatase, and bioactivity was evaluated in vivo. The results revealed that nHA/PA66/D-RADA16/bFGF reduces the degradation rate of D-RADA16 hydrogels and prolongs sustained release of bFGF, which would promote BMSCs proliferation, adhesion and osteogenesis in vitro and bone repair in vivo. Thus, it deserves more attention and is worthy of further research.
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spelling pubmed-72118822020-05-19 Controlled release of basic fibroblast growth factor from a peptide biomaterial for bone regeneration Zhao, WeiKang Li, Yuling Zhou, Ao Chen, Xiaojun Li, Kai Chen, Sinan Qiao, Bo Jiang, Dianming R Soc Open Sci Engineering Self-assembled peptide scaffolds based on D-RADA16 are an important matrix for controlled drug release and three-dimensional cell culture. In this work, D-RADA16 peptide hydrogels were coated on artificial bone composed of nano-hydroxyapatite/polyamide 66 (nHA/PA66) to obtain a porous drug-releasing structure for treating bone defects. The developed materials were characterized via transmission electron microscopy and scanning electron microscopy. The proliferation and adhesion of bone mesenchymal stem cells (BMSCs) were examined by confocal laser microscopy and CCK-8 experiments. The osteogenic ability of the porous materials towards bone BMSCs was examined in vitro by staining with Alizarin Red S and alkaline phosphatase, and bioactivity was evaluated in vivo. The results revealed that nHA/PA66/D-RADA16/bFGF reduces the degradation rate of D-RADA16 hydrogels and prolongs sustained release of bFGF, which would promote BMSCs proliferation, adhesion and osteogenesis in vitro and bone repair in vivo. Thus, it deserves more attention and is worthy of further research. The Royal Society 2020-04-01 /pmc/articles/PMC7211882/ /pubmed/32431879 http://dx.doi.org/10.1098/rsos.191830 Text en © 2020 The Authors. http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/http://creativecommons.org/licenses/by/4.0/Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.
spellingShingle Engineering
Zhao, WeiKang
Li, Yuling
Zhou, Ao
Chen, Xiaojun
Li, Kai
Chen, Sinan
Qiao, Bo
Jiang, Dianming
Controlled release of basic fibroblast growth factor from a peptide biomaterial for bone regeneration
title Controlled release of basic fibroblast growth factor from a peptide biomaterial for bone regeneration
title_full Controlled release of basic fibroblast growth factor from a peptide biomaterial for bone regeneration
title_fullStr Controlled release of basic fibroblast growth factor from a peptide biomaterial for bone regeneration
title_full_unstemmed Controlled release of basic fibroblast growth factor from a peptide biomaterial for bone regeneration
title_short Controlled release of basic fibroblast growth factor from a peptide biomaterial for bone regeneration
title_sort controlled release of basic fibroblast growth factor from a peptide biomaterial for bone regeneration
topic Engineering
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7211882/
https://www.ncbi.nlm.nih.gov/pubmed/32431879
http://dx.doi.org/10.1098/rsos.191830
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