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Homotypic targeting and drug delivery in glioblastoma cells through cell membrane-coated boron nitride nanotubes

Glioblastoma multiforme (GBM) is one of the most aggressive types of brain cancer, characterized by rapid progression, resistance to treatments, and low survival rates; the development of a targeted treatment for this disease is still today an unattained objective. Among the different strategies dev...

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Autores principales: De Pasquale, Daniele, Marino, Attilio, Tapeinos, Christos, Pucci, Carlotta, Rocchiccioli, Silvia, Michelucci, Elena, Finamore, Francesco, McDonnell, Liam, Scarpellini, Alice, Lauciello, Simone, Prato, Mirko, Larrañaga, Aitor, Drago, Filippo, Ciofani, Gianni
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7212088/
https://www.ncbi.nlm.nih.gov/pubmed/32394995
http://dx.doi.org/10.1016/j.matdes.2020.108742
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author De Pasquale, Daniele
Marino, Attilio
Tapeinos, Christos
Pucci, Carlotta
Rocchiccioli, Silvia
Michelucci, Elena
Finamore, Francesco
McDonnell, Liam
Scarpellini, Alice
Lauciello, Simone
Prato, Mirko
Larrañaga, Aitor
Drago, Filippo
Ciofani, Gianni
author_facet De Pasquale, Daniele
Marino, Attilio
Tapeinos, Christos
Pucci, Carlotta
Rocchiccioli, Silvia
Michelucci, Elena
Finamore, Francesco
McDonnell, Liam
Scarpellini, Alice
Lauciello, Simone
Prato, Mirko
Larrañaga, Aitor
Drago, Filippo
Ciofani, Gianni
author_sort De Pasquale, Daniele
collection PubMed
description Glioblastoma multiforme (GBM) is one of the most aggressive types of brain cancer, characterized by rapid progression, resistance to treatments, and low survival rates; the development of a targeted treatment for this disease is still today an unattained objective. Among the different strategies developed in the latest few years for the targeted delivery of nanotherapeutics, homotypic membrane-membrane recognition is one of the most promising and efficient. In this work, we present an innovative drug-loaded nanocarrier with improved targeting properties based on the homotypic recognition of GBM cells. The developed nanoplatform consists of boron nitride nanotubes (BNNTs) loaded with doxorubicin (Dox) and coated with cell membranes (CM) extracted from GBM cells (Dox-CM-BNNTs). We demonstrated as Dox-CM-BNNTs are able to specifically target and kill GBM cells in vitro, leaving unaffected healthy brain cells, upon successful crossing an in vitro blood-brain barrier model. The excellent targeting performances of the nanoplatform can be ascribed to the protein component of the membrane coating, and proteomic analysis of differently expressed membrane proteins present on the CM of GBM cells and of healthy astrocytes allowed the identification of potential candidates involved in the process of homotypic cancer cell recognition.
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spelling pubmed-72120882020-07-01 Homotypic targeting and drug delivery in glioblastoma cells through cell membrane-coated boron nitride nanotubes De Pasquale, Daniele Marino, Attilio Tapeinos, Christos Pucci, Carlotta Rocchiccioli, Silvia Michelucci, Elena Finamore, Francesco McDonnell, Liam Scarpellini, Alice Lauciello, Simone Prato, Mirko Larrañaga, Aitor Drago, Filippo Ciofani, Gianni Mater Des Article Glioblastoma multiforme (GBM) is one of the most aggressive types of brain cancer, characterized by rapid progression, resistance to treatments, and low survival rates; the development of a targeted treatment for this disease is still today an unattained objective. Among the different strategies developed in the latest few years for the targeted delivery of nanotherapeutics, homotypic membrane-membrane recognition is one of the most promising and efficient. In this work, we present an innovative drug-loaded nanocarrier with improved targeting properties based on the homotypic recognition of GBM cells. The developed nanoplatform consists of boron nitride nanotubes (BNNTs) loaded with doxorubicin (Dox) and coated with cell membranes (CM) extracted from GBM cells (Dox-CM-BNNTs). We demonstrated as Dox-CM-BNNTs are able to specifically target and kill GBM cells in vitro, leaving unaffected healthy brain cells, upon successful crossing an in vitro blood-brain barrier model. The excellent targeting performances of the nanoplatform can be ascribed to the protein component of the membrane coating, and proteomic analysis of differently expressed membrane proteins present on the CM of GBM cells and of healthy astrocytes allowed the identification of potential candidates involved in the process of homotypic cancer cell recognition. 2020-04-23 2020-07 /pmc/articles/PMC7212088/ /pubmed/32394995 http://dx.doi.org/10.1016/j.matdes.2020.108742 Text en http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
De Pasquale, Daniele
Marino, Attilio
Tapeinos, Christos
Pucci, Carlotta
Rocchiccioli, Silvia
Michelucci, Elena
Finamore, Francesco
McDonnell, Liam
Scarpellini, Alice
Lauciello, Simone
Prato, Mirko
Larrañaga, Aitor
Drago, Filippo
Ciofani, Gianni
Homotypic targeting and drug delivery in glioblastoma cells through cell membrane-coated boron nitride nanotubes
title Homotypic targeting and drug delivery in glioblastoma cells through cell membrane-coated boron nitride nanotubes
title_full Homotypic targeting and drug delivery in glioblastoma cells through cell membrane-coated boron nitride nanotubes
title_fullStr Homotypic targeting and drug delivery in glioblastoma cells through cell membrane-coated boron nitride nanotubes
title_full_unstemmed Homotypic targeting and drug delivery in glioblastoma cells through cell membrane-coated boron nitride nanotubes
title_short Homotypic targeting and drug delivery in glioblastoma cells through cell membrane-coated boron nitride nanotubes
title_sort homotypic targeting and drug delivery in glioblastoma cells through cell membrane-coated boron nitride nanotubes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7212088/
https://www.ncbi.nlm.nih.gov/pubmed/32394995
http://dx.doi.org/10.1016/j.matdes.2020.108742
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