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Generation of Transgenic Cloned Buffalo Embryos Harboring the EGFP Gene in the Y Chromosome Using CRISPR/Cas9-Mediated Targeted Integration

Sex control technology is of great significance in the production of domestic animals, especially for rapidly breeding water buffalo (bubalus bubalis), which served as a research model in the present study. We have confirmed that a fluorescence protein integrated into the Y chromosome is fit for sex...

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Detalles Bibliográficos
Autores principales:	Zhao, Xiuling, Nie, Junyu, Tang, Yuyan, He, Wengtan, Xiao, Kai, Pang, Chunying, Liang, Xianwei, Lu, Yangqing, Zhang, Ming
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2020
Materias:	Veterinary Science
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7212351/ https://www.ncbi.nlm.nih.gov/pubmed/32426378 http://dx.doi.org/10.3389/fvets.2020.00199

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