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B-Cell Receptor-Associated Protein 31 Negatively Regulates the Expression of Monoamine Oxidase A Via R1

B-cell receptor-associated protein 31 (Bap31) is a three trans-membrane protein of the endoplasmic reticulum (ER). Patients who have loss of function of Bap31 suffered from X-linked syndrome, such as motor and intellectual disabilities, dystonia, and sensorineural deafness. However, the underlying m...

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Autores principales: Jia, Cong-cong, Li, Guoxun, Jiang, Rui, Liu, Xia, Yuan, Qing, Le, Weidong, Hou, Yue, Wang, Bing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7212379/
https://www.ncbi.nlm.nih.gov/pubmed/32426368
http://dx.doi.org/10.3389/fmolb.2020.00064
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author Jia, Cong-cong
Li, Guoxun
Jiang, Rui
Liu, Xia
Yuan, Qing
Le, Weidong
Hou, Yue
Wang, Bing
author_facet Jia, Cong-cong
Li, Guoxun
Jiang, Rui
Liu, Xia
Yuan, Qing
Le, Weidong
Hou, Yue
Wang, Bing
author_sort Jia, Cong-cong
collection PubMed
description B-cell receptor-associated protein 31 (Bap31) is a three trans-membrane protein of the endoplasmic reticulum (ER). Patients who have loss of function of Bap31 suffered from X-linked syndrome, such as motor and intellectual disabilities, dystonia, and sensorineural deafness. However, the underlying mechanism of Bap31 on X-linked syndrome remains unclear. Here, we found that a total of 21 proteins (9 up-regulated and 12 down-regulated proteins) related with X-linked syndrome were screened from shRNA-Bap31 transfected cells with the isobaric tags for relative and absolute quantification (iTRAQ) technique. One gene with the greatest change trend, monoamine oxidase A (MAOA), was identified. MAOA expression was up-regulated by Bap31 knockdown. However, Bap31 did not affect the ubiquitination degradation of MAOA protein. Of note, Bap31 selectively regulated the expression of cell division cycle associated 7-like (R1/RAM2/CDCA7L/JPO2, a transcriptional repressor of MAOA) and the binding activity of R1 with MAOA promoter, thereby affecting MAOA expression. This study demonstrates the molecular mechanisms of Bap31 in MAOA via R1 and supports the potential function of Bap31 on X-linked syndrome.
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spelling pubmed-72123792020-05-18 B-Cell Receptor-Associated Protein 31 Negatively Regulates the Expression of Monoamine Oxidase A Via R1 Jia, Cong-cong Li, Guoxun Jiang, Rui Liu, Xia Yuan, Qing Le, Weidong Hou, Yue Wang, Bing Front Mol Biosci Molecular Biosciences B-cell receptor-associated protein 31 (Bap31) is a three trans-membrane protein of the endoplasmic reticulum (ER). Patients who have loss of function of Bap31 suffered from X-linked syndrome, such as motor and intellectual disabilities, dystonia, and sensorineural deafness. However, the underlying mechanism of Bap31 on X-linked syndrome remains unclear. Here, we found that a total of 21 proteins (9 up-regulated and 12 down-regulated proteins) related with X-linked syndrome were screened from shRNA-Bap31 transfected cells with the isobaric tags for relative and absolute quantification (iTRAQ) technique. One gene with the greatest change trend, monoamine oxidase A (MAOA), was identified. MAOA expression was up-regulated by Bap31 knockdown. However, Bap31 did not affect the ubiquitination degradation of MAOA protein. Of note, Bap31 selectively regulated the expression of cell division cycle associated 7-like (R1/RAM2/CDCA7L/JPO2, a transcriptional repressor of MAOA) and the binding activity of R1 with MAOA promoter, thereby affecting MAOA expression. This study demonstrates the molecular mechanisms of Bap31 in MAOA via R1 and supports the potential function of Bap31 on X-linked syndrome. Frontiers Media S.A. 2020-04-30 /pmc/articles/PMC7212379/ /pubmed/32426368 http://dx.doi.org/10.3389/fmolb.2020.00064 Text en Copyright © 2020 Jia, Li, Jiang, Liu, Yuan, Le, Hou and Wang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Biosciences
Jia, Cong-cong
Li, Guoxun
Jiang, Rui
Liu, Xia
Yuan, Qing
Le, Weidong
Hou, Yue
Wang, Bing
B-Cell Receptor-Associated Protein 31 Negatively Regulates the Expression of Monoamine Oxidase A Via R1
title B-Cell Receptor-Associated Protein 31 Negatively Regulates the Expression of Monoamine Oxidase A Via R1
title_full B-Cell Receptor-Associated Protein 31 Negatively Regulates the Expression of Monoamine Oxidase A Via R1
title_fullStr B-Cell Receptor-Associated Protein 31 Negatively Regulates the Expression of Monoamine Oxidase A Via R1
title_full_unstemmed B-Cell Receptor-Associated Protein 31 Negatively Regulates the Expression of Monoamine Oxidase A Via R1
title_short B-Cell Receptor-Associated Protein 31 Negatively Regulates the Expression of Monoamine Oxidase A Via R1
title_sort b-cell receptor-associated protein 31 negatively regulates the expression of monoamine oxidase a via r1
topic Molecular Biosciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7212379/
https://www.ncbi.nlm.nih.gov/pubmed/32426368
http://dx.doi.org/10.3389/fmolb.2020.00064
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