Cargando…
B-Cell Receptor-Associated Protein 31 Negatively Regulates the Expression of Monoamine Oxidase A Via R1
B-cell receptor-associated protein 31 (Bap31) is a three trans-membrane protein of the endoplasmic reticulum (ER). Patients who have loss of function of Bap31 suffered from X-linked syndrome, such as motor and intellectual disabilities, dystonia, and sensorineural deafness. However, the underlying m...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7212379/ https://www.ncbi.nlm.nih.gov/pubmed/32426368 http://dx.doi.org/10.3389/fmolb.2020.00064 |
_version_ | 1783531609165660160 |
---|---|
author | Jia, Cong-cong Li, Guoxun Jiang, Rui Liu, Xia Yuan, Qing Le, Weidong Hou, Yue Wang, Bing |
author_facet | Jia, Cong-cong Li, Guoxun Jiang, Rui Liu, Xia Yuan, Qing Le, Weidong Hou, Yue Wang, Bing |
author_sort | Jia, Cong-cong |
collection | PubMed |
description | B-cell receptor-associated protein 31 (Bap31) is a three trans-membrane protein of the endoplasmic reticulum (ER). Patients who have loss of function of Bap31 suffered from X-linked syndrome, such as motor and intellectual disabilities, dystonia, and sensorineural deafness. However, the underlying mechanism of Bap31 on X-linked syndrome remains unclear. Here, we found that a total of 21 proteins (9 up-regulated and 12 down-regulated proteins) related with X-linked syndrome were screened from shRNA-Bap31 transfected cells with the isobaric tags for relative and absolute quantification (iTRAQ) technique. One gene with the greatest change trend, monoamine oxidase A (MAOA), was identified. MAOA expression was up-regulated by Bap31 knockdown. However, Bap31 did not affect the ubiquitination degradation of MAOA protein. Of note, Bap31 selectively regulated the expression of cell division cycle associated 7-like (R1/RAM2/CDCA7L/JPO2, a transcriptional repressor of MAOA) and the binding activity of R1 with MAOA promoter, thereby affecting MAOA expression. This study demonstrates the molecular mechanisms of Bap31 in MAOA via R1 and supports the potential function of Bap31 on X-linked syndrome. |
format | Online Article Text |
id | pubmed-7212379 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-72123792020-05-18 B-Cell Receptor-Associated Protein 31 Negatively Regulates the Expression of Monoamine Oxidase A Via R1 Jia, Cong-cong Li, Guoxun Jiang, Rui Liu, Xia Yuan, Qing Le, Weidong Hou, Yue Wang, Bing Front Mol Biosci Molecular Biosciences B-cell receptor-associated protein 31 (Bap31) is a three trans-membrane protein of the endoplasmic reticulum (ER). Patients who have loss of function of Bap31 suffered from X-linked syndrome, such as motor and intellectual disabilities, dystonia, and sensorineural deafness. However, the underlying mechanism of Bap31 on X-linked syndrome remains unclear. Here, we found that a total of 21 proteins (9 up-regulated and 12 down-regulated proteins) related with X-linked syndrome were screened from shRNA-Bap31 transfected cells with the isobaric tags for relative and absolute quantification (iTRAQ) technique. One gene with the greatest change trend, monoamine oxidase A (MAOA), was identified. MAOA expression was up-regulated by Bap31 knockdown. However, Bap31 did not affect the ubiquitination degradation of MAOA protein. Of note, Bap31 selectively regulated the expression of cell division cycle associated 7-like (R1/RAM2/CDCA7L/JPO2, a transcriptional repressor of MAOA) and the binding activity of R1 with MAOA promoter, thereby affecting MAOA expression. This study demonstrates the molecular mechanisms of Bap31 in MAOA via R1 and supports the potential function of Bap31 on X-linked syndrome. Frontiers Media S.A. 2020-04-30 /pmc/articles/PMC7212379/ /pubmed/32426368 http://dx.doi.org/10.3389/fmolb.2020.00064 Text en Copyright © 2020 Jia, Li, Jiang, Liu, Yuan, Le, Hou and Wang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Molecular Biosciences Jia, Cong-cong Li, Guoxun Jiang, Rui Liu, Xia Yuan, Qing Le, Weidong Hou, Yue Wang, Bing B-Cell Receptor-Associated Protein 31 Negatively Regulates the Expression of Monoamine Oxidase A Via R1 |
title | B-Cell Receptor-Associated Protein 31 Negatively Regulates the Expression of Monoamine Oxidase A Via R1 |
title_full | B-Cell Receptor-Associated Protein 31 Negatively Regulates the Expression of Monoamine Oxidase A Via R1 |
title_fullStr | B-Cell Receptor-Associated Protein 31 Negatively Regulates the Expression of Monoamine Oxidase A Via R1 |
title_full_unstemmed | B-Cell Receptor-Associated Protein 31 Negatively Regulates the Expression of Monoamine Oxidase A Via R1 |
title_short | B-Cell Receptor-Associated Protein 31 Negatively Regulates the Expression of Monoamine Oxidase A Via R1 |
title_sort | b-cell receptor-associated protein 31 negatively regulates the expression of monoamine oxidase a via r1 |
topic | Molecular Biosciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7212379/ https://www.ncbi.nlm.nih.gov/pubmed/32426368 http://dx.doi.org/10.3389/fmolb.2020.00064 |
work_keys_str_mv | AT jiacongcong bcellreceptorassociatedprotein31negativelyregulatestheexpressionofmonoamineoxidaseaviar1 AT liguoxun bcellreceptorassociatedprotein31negativelyregulatestheexpressionofmonoamineoxidaseaviar1 AT jiangrui bcellreceptorassociatedprotein31negativelyregulatestheexpressionofmonoamineoxidaseaviar1 AT liuxia bcellreceptorassociatedprotein31negativelyregulatestheexpressionofmonoamineoxidaseaviar1 AT yuanqing bcellreceptorassociatedprotein31negativelyregulatestheexpressionofmonoamineoxidaseaviar1 AT leweidong bcellreceptorassociatedprotein31negativelyregulatestheexpressionofmonoamineoxidaseaviar1 AT houyue bcellreceptorassociatedprotein31negativelyregulatestheexpressionofmonoamineoxidaseaviar1 AT wangbing bcellreceptorassociatedprotein31negativelyregulatestheexpressionofmonoamineoxidaseaviar1 |