Cargando…

Prevalence of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae Causing Bloodstream Infections in Cancer Patients from Southwest of Iran

INTRODUCTION: This study aimed to evaluate the frequency rate of extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-PE) causing bloodstream infections (BSIs) in cancer patients referred to one of the major referral hospitals in Ahvaz city, southwest Iran. MATERIALS AND METHODS: In t...

Descripción completa

Detalles Bibliográficos
Autores principales: Abbasi Montazeri, Effat, Khosravi, Azar Dokht, Saki, Morteza, Sirous, Mehrandokht, Keikhaei, Bijan, Seyed-Mohammadi, Sakineh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7212769/
https://www.ncbi.nlm.nih.gov/pubmed/32440169
http://dx.doi.org/10.2147/IDR.S254357
_version_ 1783531675380088832
author Abbasi Montazeri, Effat
Khosravi, Azar Dokht
Saki, Morteza
Sirous, Mehrandokht
Keikhaei, Bijan
Seyed-Mohammadi, Sakineh
author_facet Abbasi Montazeri, Effat
Khosravi, Azar Dokht
Saki, Morteza
Sirous, Mehrandokht
Keikhaei, Bijan
Seyed-Mohammadi, Sakineh
author_sort Abbasi Montazeri, Effat
collection PubMed
description INTRODUCTION: This study aimed to evaluate the frequency rate of extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-PE) causing bloodstream infections (BSIs) in cancer patients referred to one of the major referral hospitals in Ahvaz city, southwest Iran. MATERIALS AND METHODS: In this study, 1700 blood cultures were collected from 610 cancer patients suspected to have BSI from October 2016 to August 2017 referred to the Shafa cancer hospital, Ahvaz, southwest of Iran. The blood culture bottles were incubated aerobically at 35–37ºC for 24 hours and then sub-cultured on routine microbiology culture media. The bacterial colonies were identified using standard tests. The antibiotic susceptibility testing was achieved by the disc-diffusion method. The phenotypic detection of ESBLs was carried out by the combination disc-diffusion test (CDDT). Finally, the polymerase chain reaction (PCR) was performed to investigate the presence of bla(TEM), bla(CTX), bla(SHV,) and bla(PER) genes. RESULTS: The prevalence of BSI in cancer patients was 16.4% (100/610). Gram-negative rods with rate of 74% (74/100) were the most prevalent bacteria. The frequency of Enterobacteriaceae family was 21% including Escherichia coli (n: 8), Klebsiella pneumoniae (n: 6), Enterobacter spp. (n: 5), Citrobacter freundii (n: 1), and Serratia marcescens (n: 1). All isolates were multidrug-resistant (resistance to three or more antibiotics). The results of CDDT showed that 42.8% (9/21) of Enterobacteriaceae isolates had a positive ESBL test of which 100% (9/9) indicated positive band for at least one of the ESBL genes by PCR method. The bla(CTX-M) and bla(TEM) genes were detected in 38% (8/21) and 23.8% (5/21) of isolates, respectively, while the bla(SHV) and bla(PER) were not detected in any isolates. CONCLUSION: Based on the results, surveillance, and antibiotic stewardship programs should be implemented for cancer patients to prevent the spread of more ESBL-PE that have limited therapeutically choices.
format Online
Article
Text
id pubmed-7212769
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Dove
record_format MEDLINE/PubMed
spelling pubmed-72127692020-05-21 Prevalence of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae Causing Bloodstream Infections in Cancer Patients from Southwest of Iran Abbasi Montazeri, Effat Khosravi, Azar Dokht Saki, Morteza Sirous, Mehrandokht Keikhaei, Bijan Seyed-Mohammadi, Sakineh Infect Drug Resist Original Research INTRODUCTION: This study aimed to evaluate the frequency rate of extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-PE) causing bloodstream infections (BSIs) in cancer patients referred to one of the major referral hospitals in Ahvaz city, southwest Iran. MATERIALS AND METHODS: In this study, 1700 blood cultures were collected from 610 cancer patients suspected to have BSI from October 2016 to August 2017 referred to the Shafa cancer hospital, Ahvaz, southwest of Iran. The blood culture bottles were incubated aerobically at 35–37ºC for 24 hours and then sub-cultured on routine microbiology culture media. The bacterial colonies were identified using standard tests. The antibiotic susceptibility testing was achieved by the disc-diffusion method. The phenotypic detection of ESBLs was carried out by the combination disc-diffusion test (CDDT). Finally, the polymerase chain reaction (PCR) was performed to investigate the presence of bla(TEM), bla(CTX), bla(SHV,) and bla(PER) genes. RESULTS: The prevalence of BSI in cancer patients was 16.4% (100/610). Gram-negative rods with rate of 74% (74/100) were the most prevalent bacteria. The frequency of Enterobacteriaceae family was 21% including Escherichia coli (n: 8), Klebsiella pneumoniae (n: 6), Enterobacter spp. (n: 5), Citrobacter freundii (n: 1), and Serratia marcescens (n: 1). All isolates were multidrug-resistant (resistance to three or more antibiotics). The results of CDDT showed that 42.8% (9/21) of Enterobacteriaceae isolates had a positive ESBL test of which 100% (9/9) indicated positive band for at least one of the ESBL genes by PCR method. The bla(CTX-M) and bla(TEM) genes were detected in 38% (8/21) and 23.8% (5/21) of isolates, respectively, while the bla(SHV) and bla(PER) were not detected in any isolates. CONCLUSION: Based on the results, surveillance, and antibiotic stewardship programs should be implemented for cancer patients to prevent the spread of more ESBL-PE that have limited therapeutically choices. Dove 2020-05-06 /pmc/articles/PMC7212769/ /pubmed/32440169 http://dx.doi.org/10.2147/IDR.S254357 Text en © 2020 Abbasi Montazeri et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Abbasi Montazeri, Effat
Khosravi, Azar Dokht
Saki, Morteza
Sirous, Mehrandokht
Keikhaei, Bijan
Seyed-Mohammadi, Sakineh
Prevalence of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae Causing Bloodstream Infections in Cancer Patients from Southwest of Iran
title Prevalence of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae Causing Bloodstream Infections in Cancer Patients from Southwest of Iran
title_full Prevalence of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae Causing Bloodstream Infections in Cancer Patients from Southwest of Iran
title_fullStr Prevalence of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae Causing Bloodstream Infections in Cancer Patients from Southwest of Iran
title_full_unstemmed Prevalence of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae Causing Bloodstream Infections in Cancer Patients from Southwest of Iran
title_short Prevalence of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae Causing Bloodstream Infections in Cancer Patients from Southwest of Iran
title_sort prevalence of extended-spectrum beta-lactamase-producing enterobacteriaceae causing bloodstream infections in cancer patients from southwest of iran
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7212769/
https://www.ncbi.nlm.nih.gov/pubmed/32440169
http://dx.doi.org/10.2147/IDR.S254357
work_keys_str_mv AT abbasimontazerieffat prevalenceofextendedspectrumbetalactamaseproducingenterobacteriaceaecausingbloodstreaminfectionsincancerpatientsfromsouthwestofiran
AT khosraviazardokht prevalenceofextendedspectrumbetalactamaseproducingenterobacteriaceaecausingbloodstreaminfectionsincancerpatientsfromsouthwestofiran
AT sakimorteza prevalenceofextendedspectrumbetalactamaseproducingenterobacteriaceaecausingbloodstreaminfectionsincancerpatientsfromsouthwestofiran
AT sirousmehrandokht prevalenceofextendedspectrumbetalactamaseproducingenterobacteriaceaecausingbloodstreaminfectionsincancerpatientsfromsouthwestofiran
AT keikhaeibijan prevalenceofextendedspectrumbetalactamaseproducingenterobacteriaceaecausingbloodstreaminfectionsincancerpatientsfromsouthwestofiran
AT seyedmohammadisakineh prevalenceofextendedspectrumbetalactamaseproducingenterobacteriaceaecausingbloodstreaminfectionsincancerpatientsfromsouthwestofiran