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Identification of shared genomic aberrations between angiomatous and microcystic meningiomas

BACKGROUND: Angiomatous and microcytic meningiomas are classified as rare subtypes of grade I meningiomas by World Health Organization (WHO). They typically exhibit distinct histopathological features as indicated by their WHO titles; however, these angiomatous and microcystic features are often int...

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Autores principales: Kuroi, Yasuhiro, Akagawa, Hiroyuki, Shibuya, Makoto, Onda, Hideaki, Maegawa, Tatsuya, Kasuya, Hidetoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7212863/
https://www.ncbi.nlm.nih.gov/pubmed/32642661
http://dx.doi.org/10.1093/noajnl/vdz028
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author Kuroi, Yasuhiro
Akagawa, Hiroyuki
Shibuya, Makoto
Onda, Hideaki
Maegawa, Tatsuya
Kasuya, Hidetoshi
author_facet Kuroi, Yasuhiro
Akagawa, Hiroyuki
Shibuya, Makoto
Onda, Hideaki
Maegawa, Tatsuya
Kasuya, Hidetoshi
author_sort Kuroi, Yasuhiro
collection PubMed
description BACKGROUND: Angiomatous and microcytic meningiomas are classified as rare subtypes of grade I meningiomas by World Health Organization (WHO). They typically exhibit distinct histopathological features as indicated by their WHO titles; however, these angiomatous and microcystic features are often intermixed. Recently, angiomatous meningiomas were reported to show characteristic chromosomal polysomies unlike the other WHO grade I meningiomas. In the present study, we hypothesize that microcystic meningiomas share similar cytogenetic abnormalities with angiomatous meningioma. METHODS: We performed copy number analysis using single nucleotide polymorphism (SNP) arrays for three angiomatous and eight microcystic meningiomas. Of these, three angiomatous and three microcystic meningiomas were also analyzed by whole exome sequencing and RNA sequencing. RESULTS: We first analyzed three angiomatous and three microcystic meningiomas for which both frozen tissues and peripheral blood were accessible. Copy number analysis confirmed previously reported multiple polysomies in angiomatous meningiomas, which were entirely replicated in microcystic meningiomas when analyzed on different analytical platforms with five additional samples prepared from formalin-fixed paraffin-embedded tumors. Polysomy of chromosome 5 was found in all cases, along with chromosome 6, 12, 17, 18, and 20 in more than half of the cases including both angiomatous and microcystic meningiomas. Furthermore, next generation sequencing did not reveal any distinctive somatic point mutations or differences in gene expression characterizing either angiomatous or microcystic meningiomas, indicating a common genetic mechanism underlying tumorigenesis. CONCLUSIONS: Angiomatous and microcystic meningiomas have substantially similar genetic profiles represented by the characteristic patterns of multiple polysomies originating from chromosome 5 amplification.
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spelling pubmed-72128632020-07-07 Identification of shared genomic aberrations between angiomatous and microcystic meningiomas Kuroi, Yasuhiro Akagawa, Hiroyuki Shibuya, Makoto Onda, Hideaki Maegawa, Tatsuya Kasuya, Hidetoshi Neurooncol Adv Basic and Translational Investigations BACKGROUND: Angiomatous and microcytic meningiomas are classified as rare subtypes of grade I meningiomas by World Health Organization (WHO). They typically exhibit distinct histopathological features as indicated by their WHO titles; however, these angiomatous and microcystic features are often intermixed. Recently, angiomatous meningiomas were reported to show characteristic chromosomal polysomies unlike the other WHO grade I meningiomas. In the present study, we hypothesize that microcystic meningiomas share similar cytogenetic abnormalities with angiomatous meningioma. METHODS: We performed copy number analysis using single nucleotide polymorphism (SNP) arrays for three angiomatous and eight microcystic meningiomas. Of these, three angiomatous and three microcystic meningiomas were also analyzed by whole exome sequencing and RNA sequencing. RESULTS: We first analyzed three angiomatous and three microcystic meningiomas for which both frozen tissues and peripheral blood were accessible. Copy number analysis confirmed previously reported multiple polysomies in angiomatous meningiomas, which were entirely replicated in microcystic meningiomas when analyzed on different analytical platforms with five additional samples prepared from formalin-fixed paraffin-embedded tumors. Polysomy of chromosome 5 was found in all cases, along with chromosome 6, 12, 17, 18, and 20 in more than half of the cases including both angiomatous and microcystic meningiomas. Furthermore, next generation sequencing did not reveal any distinctive somatic point mutations or differences in gene expression characterizing either angiomatous or microcystic meningiomas, indicating a common genetic mechanism underlying tumorigenesis. CONCLUSIONS: Angiomatous and microcystic meningiomas have substantially similar genetic profiles represented by the characteristic patterns of multiple polysomies originating from chromosome 5 amplification. Oxford University Press 2019-09-28 /pmc/articles/PMC7212863/ /pubmed/32642661 http://dx.doi.org/10.1093/noajnl/vdz028 Text en © The Author(s) 2019. Published by Oxford University Press, the Society for Neuro-Oncology and the European Association of Neuro-Oncology. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Basic and Translational Investigations
Kuroi, Yasuhiro
Akagawa, Hiroyuki
Shibuya, Makoto
Onda, Hideaki
Maegawa, Tatsuya
Kasuya, Hidetoshi
Identification of shared genomic aberrations between angiomatous and microcystic meningiomas
title Identification of shared genomic aberrations between angiomatous and microcystic meningiomas
title_full Identification of shared genomic aberrations between angiomatous and microcystic meningiomas
title_fullStr Identification of shared genomic aberrations between angiomatous and microcystic meningiomas
title_full_unstemmed Identification of shared genomic aberrations between angiomatous and microcystic meningiomas
title_short Identification of shared genomic aberrations between angiomatous and microcystic meningiomas
title_sort identification of shared genomic aberrations between angiomatous and microcystic meningiomas
topic Basic and Translational Investigations
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7212863/
https://www.ncbi.nlm.nih.gov/pubmed/32642661
http://dx.doi.org/10.1093/noajnl/vdz028
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