MPC-12 ACCURACY OF INTRAOPERATIVE SIMPLE FLOW CYTOMETER FOR HIGH GRADE GLIOMA OPERATION COMPARED WITH INTRAOPERATIVE FROZEN DIAGNOSIS

INTRODUCTION: Intraoperative simple flow cytometer (iFC) was developed in recently, we examined the correlation intraoperative frozen diagnosis (IOFD) and intraoperative frozen section of resection margin (IOFM) to iFC. METHOD AND MATERIAL: Total 33 cases of high grade glioma patients were underwent operation in April 2017 ~ May 2019, and IOFD and IOFM were compared to iFC. Sample of iFC were retrieved just same parts of IOFM and IOFD. We compared to microscopical findings, MIB-1, malignant index (MI), and MI Level (G0-4). RESULTS: Accuracy rate of iFC and IOFD in high grade glioma was 84%, and the concordance rate of grading was17.2%. Correlation coefficients between MIB-1 and iFC of IOFD was r=0.5019 P=0.0065. Accuracy rate of IOFM was 46.8%, and concordance rate of grading was 35.5%. Correlation coefficients between MIB-1 and iFC of IOFM was r=0.5899 P=0.0001. CONCLUSIONS: Although iFC accuracy rates iFC of IOFD and IOFM were high, iFC concordance rates and grade were low. Correlation between MIB-1 and iFC of IOFM was better than that of IOFD. Probably, iFC of IOFD was a little difficult because of sample heterozygosity.